Investigating Potential Protective Effects of Rutin against Deltametrin-Induced Spleen Toxicity in Rats through Oxidative Stress, Apoptosis and Inflammation Markers

Abstract

In this study, the protective effects of rutin (RUT) against spleen toxicity caused by deltamethrin (DLM) were investigated. For this purpose, DLM and/or RUT were applied to Sprague dawley rats for 28 days. According to results, DLM increased malondialdehyde (MDA) levels in spleen tissue, decreased glutathione (GSH) levels and mRNA transcript levels of superoxide dismutase (SOD), catalase (KAT) and glutathione peroxidase (GPx) enzymes. RUT treatment caused a decrease in MDA levels, activated SOD, KAT and GPx expressions and increased GSH levels. Also, RUT showed anti-inflammatory effect in spleen tissue by suppressing the expressions of mitogen-activated protein kinase-14 (MAPK14), nuclear factor kappa-B (NF-ĸB) and interleukin-1-beta (IL-1β) activated by DLM. It was observed that the expression of anti-apoptotic protein B-cell lymphoma-2 (Bcl-2) suppressed by DLM was activated by RUT. It is also among the other findings that the apoptotic protein Bcl-2-related X protein (Bax) is activated by DLM and suppressed by RUT. As a result, it was determined that DLM had a toxic effect on spleen tissue by causing oxidative stress, inflammation and apoptosis, however, RUT demonstrated antioxidant, anti-inflammatory and anti-apoptotic effects and protected spleen tissue from the toxic effect of DLM.

Keywords

• Oxidative stress
• Inflammation
• Apoptosis
• Deltamethrin
• Rutin

Ratlarda Deltametrin’in Neden Olduğu Dalak Toksisitesine Karşı Rutin’in Potansiyel Koruyucu Etkilerinin Oksidatif Stres, Apoptoz ve İnflamasyon Belirteçleri Üzerinden Araştırılması

Öz

Çalışmada deltametrinin (DLM) meydana getirdiği dalak toksisitesine karşı rutin’in (RUT) koruyucu etkileri araştırılmıştır. Bu amaçla çalışmada Sprague dawley ratlara 28 gün boyunca DLM ve/veya RUT uygulandı. DLM’nin dalak dokusunda malondialdehit (MDA) seviyelerini arttırdığı, glutatyon (GSH) seviyelerini ve süperoksit dismutaz (SOD), katalaz (KAT) ve glutatyon peroksidaz (GPx) enzimlerinin mRNA transkript seviyelerini ise azalttığı belirlendi. RUT tedavisinin ise MDA seviyelerinde azalma meydana getirdiği, SOD, KAT ve GPx ekspresyonlarını aktive ettiği ve GSH seviyelerini arttırdığı görüldü. Ayrıca RUT’un DLM tarafından aktive edilen mitojenle aktifleştirilen protein kinaz 14 (MAPK14), nükleer faktör kappa B (NF-ĸB) ve interlökin 1 beta (IL-1β) ekspresyonlarını baskılayarak dalak dokusunda anti-enflamatuar etki gösterdiği belirlendi. DLM tarafından baskılanan anti-apoptotik protein B hücreli lenfoma-2 (Bcl-2) ekspresyonunun RUT tarafından aktive edildiği görüldü. Ayrıca apoptotik protein olan Bcl-2 ile ilişkili X proteininin (Bax) DLM tarafından aktive edildiği, RUT tarafından ise baskılandığı elde edilen diğer bulgulardandır. Sonuç olarak DLM’nin oksidatif strese, enflamasyona ve apoptoza neden olarak dalak dokusunda toksik etki gösterdiği bununla birlikte RUT’un antioksidan, anti-enflamatuar ve anti-apoptotik etki göstererek dalak dokusunu DLM’nin toksik etkisinden koruduğu belirlendi.

Anahtar Kelimeler

• Oksidatif stres
• İnflamasyon
• Apoptoz
• Deltametrin
• Rutin

Kaynakça

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