Malignant Meme Tümörlü Köpeklerde Plazma IGF-2 Konsantrasyonu ve Tümör Dokusundaki Ekspresyonu

Yıl 2016, Cilt: 11 Sayı: 3, 305 - 313, 30.12.2016

Elvan Anadol

https://doi.org/10.17094/ataunivbd.282987

Öz

Bu çalışmanın amacı, malignant meme tümörlü köpeklerde insülin
benzeri büyüme faktörü-2 (IGF-2)'nin plazma konsantrasyonu ve tümör dokusundaki
ekspresyonunu değerlendirmektir. 
Çalışmada toplam 15 köpek kullanıldı. Sekiz meme tümörlü köpek grup 1'i,
7 sağlıklı köpek ise grup 2'yi oluşturdu. Köpeklerin diöstrus döneminde
oldukları vaginal sitolojik muayene ile belirlendi. Abdominal organların
ultrasonografik muayenesi ve toraksın radyografik bakısı yapılarak metastaz
değerlendirilmesi yapıldı. Köpeklerden kan örnekleri alındı, plazmaları elde
edildi ve IGF-2 konsantrasyonu ELISA yöntemi ile belirlendi. Grup 1'de,
tümörler “Dünya Sağlık Örgütü” (WHO)'nun belirlediği “Klinik Derecelendirme
Sistemi”ne göre sınıflandırıldı ve komple unilateral mastektomi tekniğiyle
uzaklaştırıldı. Tümörlerin histolojik olarak malignite derecesi Nottingham
yöntemine göre tubul formasyonu, nüklear pleomorfizm ve mitotik hücre sayısına
bağlı olarak değerlendirildi. Histopatolojik incelemelerde 4 karsinosarkom, 1
intraduktal papillar karsinom, 1 karsinom, 1 kompleks tip karsinom ve 1 miks
tip karsinom tanısı konuldu. İmmunohistokimyasal incelemede tümör dokusunda
meme asinus epitel hücreleri, miyoepitel hücreleri ve bazı damar düz kas
hücrelerinde IGF-2 ekspresyonunun olduğu, duktus epitel hücrelerinde ise
olmadığı gözlendi. Sağlıklı köpeklere göre, malignant meme tümörlü köpeklerde
plazma IGF-2 konsantrasyonundaki artış istatistiksel olarak anlamlı bulundu
(P˂0.05).

Anahtar Kelimeler

: IGF-2, Köpek, Malignant meme tümörü

Kaynakça

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