Sığırlarda Gebelikle İlişkili Glikoproteinlerin Tespitinde Kullanılan İki Test Kitinin Karşılaştırılması

Yıl 2017, Cilt: 12 Sayı: 1, 45 - 53, 30.04.2017

Mehmet Salih Kaya

https://doi.org/10.17094/ataunivbd.309772

Cited By: 2

Öz

Bu çalışmada;
sığırlarda bir gebelik biyo-markırı olan gebelik ilişkili glikoproteinlerin kan
plazmasında tespitine yönelik hazırlanan farklı iki ticari gebelik tanı kitinin
(Sığır Gebelik Test Kiti ve Görsel Gebelik Test Kiti) erken gebelik tanısındaki
etkinliklerinin karşılaştırılması amaçlandı. Bu amaçla inek (n=32) ve
düvelerden (n=37) tohumlama sonrası 27-30. günde kan örneği alındı. Kan
örneklerinden toplanan plazma örnekleri, Sığır Gebelik Test Kiti ve Görsel
Gebelik Test Kitleriyle üretici firma talimatlarına göre analiz edildi. İnek ve
düvelerin gebelik durumları kan örneklerinin alımından 10 gün sonra transrektal
ultrasonografi ile tespit edildi. Gebeliklerin devamlılığı ise tohumlama
sonrası 60. günde yapılan ikinci transrektal ultrasonografi ile teyit edildi.
Sığır Gebelik Test Kiti ve Görsel Gebelik Test Kitlerinin sırasıyla;
sensitiviteleri %80 - %80, spesiviteleri %91.2 - %85.3, pozitif prediktif
değerleri %90.3 - %84.8, negatif prediktif değerleri %81.6 - %80.6, ve doğruluk
oranları %85.5 - %82.6 oldu. Sonuç olarak; Sığır Gebelik Test Kiti ve Görsel
Gebelik Test Kitinin inek ve düvelerde erken gebelik tanısında etkinliklerinin
benzer olduğu ve rutin erken gebelik tespitinde güvenle kullanılabileceği
kanısına varıldı.

Anahtar Kelimeler

ELISA, Erken gebelik tanısı, Gebelik ilişkili glikoproteinler, Görsel gebelik test kiti, İnek

Comparison of Two Test Kits for the Detection of Pregnancy Associated Glycoproteins in Cattle

Öz

In this study, it was aimed to
compare the efficiency of two commercial pregnancy test kits (Bovine Pregnancy
Test Kit and Visual Pregnancy Test Kit) based on detection of pregnancy
associated glycoproteins, which are pregnancy bio-markers in cattle for early
pregnancy diagnosis in the blood plasma of cattle. For this purpose blood
samples from dairy cows (n=32) and heifers (n=37) were collected between 27 and
30 days after insemination. Plasma samples collected
from blood samples were analyzed with Bovine Pregnancy Test Kit and Visual
Pregnancy Test Kit according to manufacturer’s protocols. Pregnancy status of
cows and heifers was diagnosed ten days after blood samples collection by using
transrectal ultrasonography. The pregnancy was confirmed by second transrectal
ultrasonography at 60 days post insemination. The sensitivity, specificity,
positive predictive value, negative predictive value and accuracy rates of the
Bovine Pregnancy Test Kit and Visual Pregnancy Test Kit were 80% - 80%, 91.2% -
85.3%, 90.3% - 84.8%, 81.6% - 80.6%, and 85.5% - 82.6%, respectively. As a
result; it was concluded that Bovine Pregnancy Test Kit and Visual Pregnancy Test
Kit had similar efficacies in early pregnancy diagnosis and could be used
safely for routine early pregnancy diagnosis in cows and heifers.

Anahtar Kelimeler

Cattle, Early pregnancy diagnosis, ELISA, Pregnancy-associated glycoproteins, Visual pregnancy test kit

Kaynakça

• 1. Inal S., Cam M., 2015. Culling and Replacement in Dairy Cattle Herds. Turkiye Klinikleri J Vet Sci Obstet Gynecol-Special Topics, 1, 106-112.
• 2. Ott TL., Dechow C., O’Connor ML., 2014. Advances in reproductive management: pregnancy diagnosis in ruminants. Anim Reprod, 11, 207-216.
• 3. Whitlock BK., Maxwell HS., 2008. Pregnancy-associated glycoproteins and pregnancy wastage in cattle. Theriogenology, 70, 550-559.
• 4. Balhara AK., Gupta M., Singh S., Mohanty AK., Singh I., 2013. Early pregnancy diagnosis in bovines: current status and future directions. Sci World J, 1-10.
• 5. Peters AT., 2013. Bovine placenta: A review on morphology, components, and defects from terminology and clinical perspectives. Theriogenology, 80, 693-705.
• 6. Romano JE., Thompson JA., Kraemer DC., Westhusin ME., Forrest DW., Tomaszweski MA., 2007. Early pregnancy diagnosis by palpation per rectum: Influence on embryo/fetal viability in dairy cattle. Theriogenology, 67, 486-493. 7. Romano JE., Thompson JA., Forrest DW., Westhusin ME., Tomaszweski MA., Kraemer DC., 2006. Early pregnancy diagnosis by transrectal ultrasonography in dairy cattle. Theriogenology, 66, 1034-1041.
• 8. Nak D., Nak Y., Başaran DA., Keskin A., 2005. İneklerde transrektal ultrasonografi, serum progesteron analizi ve vaginal smear muayenesi ile erken gebelik tanısı. Vet Bil Derg, 21, 39-44.
• 9. Kose M., Tekeli T., 2014. Effect of ovarian functions on estrus sychronization and pregnancy rate in Brown Swiss heifers and non-lactating cows. Eurasian J Vet Sci, 30, 53-58.
• 10. Patel OV., Sulon J., Beckers JF., Takahashi T., Hirako M., Sasaki N., Domeki I., 1997. Plasma bovine pregnancy-associated glycoprotein concentrations throughout gestation in relationship to fetal number in the cow. Eur J Endocrinol, 137, 423-428.
• 11. Lopez-Gatius F., Hunter RHF., Garbayo JM., Santolaria P., Yaniz J., Serrano B., Ayad A., de Sousa NM., Beckers JF., 2007. Plasma concentrations of pregnancy-associated glycoprotein-1 (PAG-1) in high producing dairy cows suffering early fetal loss during the warm season. Theriogenology, 67, 1324-1330.
• 12. Friedrich M., Holtz W., 2010. Establishment of an ELISA for measuring bovine pregnancy-associated glycoprotein in serum or milk and its application for early pregnancy detection. Reprod Domest Anim, 45, 142-146.
• 13. LeBlanc SJ., 2013. Short communication: Field evaluation of a pregnancy confirmation test using milk samples in dairy cows. J Dairy Sci, 96, 2345-2348.
• 14. Zoli AP., Guilbault LA., Delahaut P., Ortiz WB., Beckers JF., 1992. Radioimmunoassay of a pregnancy associated glycoprotein in serum: Its application for pregnancy diagnosis. Biol Reprod, 46, 83-92.
• 15. Green JA., Parks TE., Avalle MP., Telugu BP., McLain AL., Peterson AJ., McMillan M., Mathialagan N., Hook RR., Xie S., Roberts RM., 2005. The establishment of an ELISA for the detection of pregnancy-associated glycoproteins (PAGs) in the serum of pregnant cows and heifers. Theriogenology, 63, 1481-1503.
• 16. Kastelic JP., 2006. Critical evaluation of scientific articles and other sources of information: an introduction to evidence-based veterinary medicine. Theriogenology, 66, 534-542.
• 17. Karen A., De Sousa NM., Beckers JF., Bajcsy AC., Tibold J., Mádle I., Szencia O., 2015. Comparison of a commercial bovine pregnancy-associated glycoprotein ELISA test and a pregnancy-associated glycoprotein radiomimmunoassay test for early pregnancy diagnosis in dairy cattle. Anim Reprod Sci, 159, 31-37.
• 18. Silva E., Sterry RA., Kolb D., Mathialagan N., McGrath M.F, Ballam JM., Fricke PM., 2007. Accuracy of a pregnancy-associated glycoprotein ELISA to determine pregnancy status of lactating dairy cows twenty-seven days after timed artificial insemination. J Dairy Sci, 90, 4612-4622.
• 19. Piechotta M., Bollwein J., Friedrich M., Heilkenbrinker T., Passavant C., Branen J., Sasser G., Hoedemaker M., Bollwein H., 2011. Comparison of commercial ELISA blood tests for early pregnancy detection in dairy cows. J Reprod Dev, 57, 72-75.
• 20. Ricci A., Carvalho PD., Amundson MC., Fourdraine RH., Vincenti L., Fricke PM., 2015. Factors associated with pregnancy-associated glycoprotein (PAG) levels in plasma and milk of Holstein cows during early pregnancy and their effect on the accuracy of pregnancy diagnosis. J Dairy Sci, 98, 2502-2514.
• 21. Kaya MS., Kose M., Bozkaya F., Mutlu H., Ucar EH., Atlı MO., 2016. Early pregnancy diagnosis by using a commercial ELISA test based on pregnancy associated glycoproteins in heifers and lactating cows. Turk J Vet Anim Sci, 40, 694-699.
• 22. Karen A., Darwish S., Ramoun A., Tawfeek K., Van Hanh N., De Sousa M., Sulon J., Szenci O., Beckers JF., 2007. Accuracy of ultrasonography and pregnancy–associated glycoprotein test for pregnancy diagnosis in buffaloes. Theriogenology, 68, 1150-1155.
• 23. Fernandez IG., Moncebaez J., Elizondo C., Hernandez H., Ulloa-Arvizu R., Rojas S., 2013. Negative prediction value of pregnancy-associated glycoprotein contributes to reduce the days during which nonpregnant holstein cows are subjected to diverse strategies of hormonal synchronization. Int J Anim Vet Adv, 5, 160-164.
• 24. Szenci O., Beckers JF., Humblot P., Sulon J., Sasser G., Taverne MA., Varga J., Baltusen R., Schekk G., 1998. Comparison of ultrasonography, bovine pregnancy-specific protein B, and bovine pregnancy-associated glycoprotein 1 tests for pregnancy detection in dairy cows. Theriogenology, 50, 77-88.
• 25. Shahin M., Friedrich M., Gauly M., Holtz W., 2014. Pregnancy-associated glycoprotein (pag) profile of holstein–friesian cows as compared to dual-purpose and beef cows. Reprod Domest Anim, 49, 618-620.
• 26. Green JC., Volkmann DH., Poock SE., McGrath MF., Ehrhardt M., Moseley AE., Lucy MC., 2009. Technical note: A rapid enzyme-linked immunosorbent assay blood test for pregnancy in dairy and beef cattle. J Dairy Sci, 92, 3819-3824.
• 27. Dinc DA., 2015. Dairy Herd Health Concept and Role of the Veterinarian. J Vet Sci Obstet Gynecol-Special Topics, 1, 1-16.
• 28. Humblot P., 2001. Use of pregnancy specific proteins and progesterone assays to monitor pregnancy and determine the timing, frequencies and sources of embryonic mortality in ruminants. Theriogenology, 56, 1417-1433.
• 29. Silke V., Diskin MG., Kenny DA., Boland MP., Dillon P., Mee JF., Sreenan JM., 2001. Extent, pattern and factors associated with late embryonic loss in dairy cows. Anim Reprod Sci, 15, 1-12.
• 30. Perenyi Z., Szenci O., Sulon J., Drion PV., Beckers JF., 2002. Comparison of the ability of three radioimmunoassay to detect pregnancy-associated glycoproteins in bovine plasma. Reprod Domest Anim, 37, 100-104.