Koyun ve Keçi Sütlerinde Coxiella burnetii Varlığının PCR ile Araştırılması

Yıl 2017, Cilt: 12 Sayı: 2, 152 - 156, 30.10.2017

Ayşe Kılıç

https://doi.org/10.17094/ataunivbd.347968

Öz

Q
humması, büyük ölçüde geviş getiren hayvanların süt, vajinal mukus ve dışkı
gibi ürünlerle dışarıya saçtığı Coxiella
burnetii bakterisinin neden olduğu zoonotik bir hastalıktır. Sığır, koyun
ve keçiler etkenin insanlara bulaşmasında primer rezervuar konakçılardır. Bu çalışmada
abort yapan ve yapmayan farklı koyun ve keçi sürülerinden toplanan 120 süt
örneği, C.burnetii suşunun transpozon repetitive gen bölgesinden türetilen tür spesifik Trans 1 ve 2
primerleri kullanılarak PZR amplifikasyonuna maruz bırakıldı. Elazığ ve komşu
illerden toplanan sütlerde abort yapmış koyunlarda %10 ve abort yapmış
keçilerde ise %3.33 oranında C.burnetii
DNA’sı PZR ile pozitif olarak tespit edildi. Abort yapmayan sürülerden alınan
koyun ve keçi sütü örneklerinde PZR testi ile pozitiflik saptanmadı. Sonuç
olarak abortus öyküsü olan ve olmayan koyun ve keçi sürülerinden toplanan süt
örneklerinde C.burnetii
enfeksiyonunun moleküler yöntemlerle araştırılması amaçlanmıştır.

Anahtar Kelimeler

C. burnetii, PZR, Süt

The Investigation of Presence Coxiella burnetii in Sheep and Goat Milk by the PCR

Öz

Q fever is a zoonotic disease caused by Coxiella burnetii bacteria, which shedding
into largely ruminant animals with products such as milk, vaginal mucus and
stools. Cattle, sheep and goats are primary C. burnetii reservoir hosts.
In this study, 120 milk samples were collected from different sheep and goat
herds which aborting and non-aborting and A PCR was performed with Trans 1 and
Trans 2 primers derived from tranposon repetitive gen region.  The milk samples obtained from herds
belonging aborted sheep in Elazig and
neighboring provinces showed a total of 10% were detected positivity, whereas the 3.33%  milk sample taken from aborted goat herds
were observed C. burnetii as positive
by the PCR. The milk samples taken from non-aborted sheep and goat herds
weren’t observed positivity by the PCR test. As a result, it is aimed to
investigate C.burnetii infection in
goat and sheep milk samples obtained from belonging aborted and non-aborted
flocks by molecular methods.

Anahtar Kelimeler

C.burnetii, Milk, PCR

Kaynakça

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