Ratlarda Methotrexate Kaynaklı Karaciğer Toksisitesine Karşı Silymarin ve Naringin’in Yararlı Etkileri

Yıl 2017, Cilt: 12 Sayı: 2, 167 - 177, 30.10.2017

Cüneyt Çağlayan

https://doi.org/10.17094/ataunivbd.347970

Cited By: 4

Öz

Karaciğer
toksisitesi, kemoterapötik bir ilaç olan methotrexate (MTX) terapisinin
komplikasyonlarının sonucunda oluşur. Silymarin 
(SLY) ve naringin (NRG) antioksidant, anti-inflamatuvar ve
anti-hiperlipidemik gibi birçok farmakolojik özelliklere sahip biyoflavonoidlerdir.
Bu çalışma ratlarda MTX kaynaklı karaciğer toksisitesi üzerine SLY ve NRG’in
yararlı etkilerinin araştırılması için yapılmıştır. Ratlara (20 mg/kg) tek doz
periton içi MTX verildikten sonra 7 gün boyunca (25 ve 50 mg/kg) SLY ve (50 ve
100 mg/kg) NRG tedavisi oral yoldan gavaj ile verilmiştir. MTX; süperoksit
dismutaz (SOD), katalaz (KAT) ve glutatyon peroksidaz (GPx) gibi antioksidan
enzim aktivitelerini ve glutatyon (GSH) seviyesini azaltıp, lipid
peroksidasyonunu artırarak oksidatif hasarı tetiklediği belirlenmiştir. Üstelik
MTX toksikasyonu aspartat amino transferaz (AST), alanin amino transferaz (ALT)
ve alkalin fosfataz (ALP)  gibi karaciğer
enzim aktivitelerini artırmıştır. Diğer taraftan SLY ve NRG tedavisi GSH
seviyesini ve antioksidan enzim aktivitelerini artırıp, lipid peroksidasyonunu
inhibe etmiştir. Ayrıca SLY ve NRG tedavisi, MTX grubu ile karşılaştırıldığında
karaciğer enzim aktivitelerini azalttığı belirlenmiştir. Bu çalışmada SLY ve
NRG, MTX’ın neden olduğu karaciğer toksisitesine karşı yararlı etki
sağlamıştır.

Anahtar Kelimeler

Karaciğer enzimleri, Methotrexate, Naringin, Oksidatif stress, Silymarin

Beneficial Effects of Silymarin and Naringin Against Methotrexate-induced Hepatotoxicity in Rats

Öz

Hepatotoxicity occurs as a
result of the complications of methotrexate (MTX) therapy, which is a
chemotherapeutic drug. Silymarin (SLY) and naringin (NRG) are bioflavonoids
possess multiple pharmacological properties such as antioxidant,
anti-inflammatory and anti-hyperlipidemic activity. This study was undertaken
to investigate the beneficial effects of SLY and NRG on MTX-induced liver
toxicity in rats. After a single dose of intraperitoneal MTX (20 mg/kg) was
given to rats, SLY (25 and 50 mg/kg) and NRG (50 and 100 mg/kg) treatment was
given by oral gavage for 7 days. It has been determined that MTX induces
oxidative stress by increasing lipid peroxidation, decreased in glutathione
(GSH) level and antioxidant enzyme activities such as superoxide dismutase
(SOD), catalase (CAT) and glutathione peroxidase (GPx). Moreover, MTX
toxication has increased liver enzyme activities such as aspartate
aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase
(ALP). On the other hand, SLY and NRG treatment increased the level of
glutathione (GSH) and antioxidant enzyme activities and inhibited lipid
peroxidation. It has also been determined that SLY and NRG treatment reduces
liver enzyme activities when compared to the MTX group. In this study, SLY and
NRG provided a beneficial effect against MTX-induced liver toxicity.

Anahtar Kelimeler

Liver enzymes, Methotrexate, Naringin, Oxidative stress, Silymarin

Kaynakça

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