Ratlarda Cyclophosphamide ile İndüklenen Nefrotoksisitede Bazı Hematolojik Parametreler ve Böbreğin Histopatolojisi Üzerine Naringinin Protektif Etkileri

Yıl 2018, Cilt: 13 Sayı: 2, 219 - 228, 25.10.2018

Yusuf Dağ Emin Şengül , Muzaffer Selçuk , Serkan Yıldırım , Fikret Çelebi , Ali Çınar

https://doi.org/10.17094/ataunivbd.384225

Cited By: 3

Öz

Bu çalışmada cyclophosphamide (CYP) ile indüklenen nefrotoksisitede Naringin’in protektif etkilerinin araştırılması

amaçlanmıştır. Araştırmamızda yaklaşık 200-250 g ağırlığında, 40 adet erkek erişkin Sprague Dawley ırkı rat kullanıldı ve 5

grup oluşturuldu. Hematolojik ve biyokimyasal parametrelerin ölçümü Abocus Junior Vet5 hemogram cihazında rat modunda

ve Modüler PP otoanalizöründe yapıldı. CYP’li gruplarda alyuvar sayısı, akyuvar sayısı, hematokrit değer, trombosit sayısı ve

lenfosit (%) düşmesine rağmen, nötrofil (%) ve monosit (%) miktarı arttı (P<0.05). CYP gruplarında potasyum (K) ve sodyum

(Na) düzeyinde istatistiki anlamda (P<0.05) düşüş görülürken, fosfor (P), magnezyum (Mg), klor (CI) ve kalsiyum (Ca)’da düşüş

görülmedi. Böbrek fonksiyon testlerinden glikoz, kreatin ve ürik asit seviyeleri istatistiki açıdan anlamlı bir şekilde yükselirken

(P<0.05), üre, BUN ve laktat dehidrojenaz (LDH) değerlerindeki yükseliş ise anlamsız bulundu. Histopatolojik incelemede;

CYP’nin etkisiyle böbrek dokusu tubul epitellerinde şiddetli hidropik dejenerasyon ve koagulasyon nekrozu, Bowman

kapsülünde dilatasyon, intersitisyel damarlarda ve glomeruluslarda hiperemi belirlendi. Böbrekte oluşan bu hasarların

Naringin’in sitoprotektif etkisiyle doza bağlı olarak azaldığı saptandı. Naringin’in farklı dozlarının, CYP’nin olumsuz etkilerini

ve nefrotoksisiteyi baskılama etkisine sahip olduğu tespit edildi.

Anahtar Kelimeler

CYP, Kan Parametreleri, Naringin, Nefrotoksisite, Rat.

The Protective Effects of Naringin on Some Blood Paremeters and Kidney Histopathology in Cyclophosphamide Induced Nephrotoxicity in Rats

Öz

In this study, it was aimed to investigate the protective effects of Naringin on nephrotoxicity by Cyclophosphamide

(CYP)-induced rats. In our study, forty male adult Sprague Dawley rat weighing approximately 200-250 g were used and 5

gropus formed. Measurement of hematologic and biochemical parameters were done in the rat mode on the Abocus Junior

Vet 5 hemogram and on Modular PP autoanalyzer instruments. In the CYP groups, Neutrophil (%) and monocyte (%) increase

despite red blood cell count, white blood cell count, hematocrit value, thrombocyte count and lymphocyte (%) decrease

(P<0.05). While there were a decrease in potasium and sodium levels (P<0.05), but no decrease in other parameters such as

phosphorus, magnesium, chlorine and calcium. Altough glucose, creatinine and uric acid levels from renal function tests were

statistically significantly increased at (P<0.05), the increase in urea, BUN and lactate dehydrogenase (LDH) values were not

significant. In the histopathological exemination; It was determined that severly hydropic degeneration and coagulation

necrosis in tubule ephitelia, dilatation in Bowman capsule, hyperemia in interstitial vein and glomerulus of kidney tissue by

the effects of CYP. These damages in the kidney were found to be reduced as dose-dependent due to cytoprotective effects

of Naringin. It was determined that different doses of Naringin suppress nephrotoxicity and the negative effect of CYP.

Anahtar Kelimeler

CYP, Heamatological Parameters, Naringin, Nephrotoxicity, Rat

Kaynakça

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