Van ilinde Coenurus cerebralis’in Yaygınlığı ve Coenurosis’in Teşhisinde Yardımcı Bir Parametre Olarak Enolaz (NSE) Enziminin Önemi

Yıl 2019, Cilt: 14 Sayı: 2, 185 - 192, 25.10.2019

Kamile Biçek , Ayşe Karakuş Mustafa Serdar Değer

Öz

Bu çalışmada, Van ilindeki koyunlarda Coenurus cerebralis’in yaygınlığı ve mevsimsel insidensi ve coenurosisli hayvanlarda kan serumu Enolaz enzim seviyeleri ELISA testi ile araştırılmıştır. İncelenen hayvanların %64.7’si (241 koyunun 156’sı) C. cerebralis yönünden pozitif bulunmuştur. Enfeksiyon oranı koçlarda %35.7, dişi koyunlarda %29 olarak tespit edilmiştir. Coenurosis, 0-1 yaş aralığındaki koyunlarda %46.9 olarak görülürken 2 yaş üstündeki hayvanlarda bu oran %17.8 olarak belirlenmiştir. C. cerebralis kistlerinin %32.1 sağ, %46.8’nin sol hemisferine, %12.2’ sinin beyinin her iki yarımına birden, %5.1’inin cerebelluma ve occipital bölgeye ise %3.8 oranında yerleştikleri gözlemlenmiştir. Kistlerin beynin her iki lobunda birden yaygın olarak yalnızca erkeklerde gözlenmesi (dişilerde 0, erkeklerde 19 adet kist) istatistiksel olarak önemli bulunmuştur (P<0.05). C. cerebralis’in en yüksek Kasım ayında (%10.4), en az ise Mayıs ayında (%2.1) görüldüğü tespit edilmiştir. İncelenen toplam 80 (40 dişi, 40 erkek koyun) hayvanın kan serumlarındaki Enolaz seviyeleri 5.44–84.96 μg/l olarak belirlenmiştir. Klinik belirti gösteren hayvanların tamamında Enolaz enzimi yüksek bulunurken; semptom göstermeyenlerde sonuçlar Cut-off sınırları içerisinde olduğu görülmüştür. Sonuçlar istatistiksel olarak anlamlı bulunmuştur (P<0.05). Ayrıca istatiksel olarak dişi ve erkekler arasında anlamlı bir fark bulunamamıştır. Sonuçta Enolaz enziminin, coenurosiste klinik belirtileri gösteren koyunlarda teşhiste yardımcı bir parametre olarak kullanılabileceği kanaatine varılmıştır.

Anahtar Kelimeler

Coenurus cerebralis, Enolaz, Koyun, Prevalans, Taenia multiceps

Prevalence of Coenurus Cerebralis in Van, Turkey and the Significance of the Enolase (NSE) Enzyme as a Supporting Parameter in the Diagnosis of Coenurosis

Öz

This study investigated the prevalence of Coenurus cerebralis in sheep in the province of Van, Turkey, its seasonal incidence and serum Enolase enzym levels with ELISA method. 64.7% of the examined animals (156 out of 241 sheep) were found to be positive in terms of C. cerebralis. The rate of infection was found to be 35.7% in male and 29% in female sheep. While coenurosis was seen by 46.9% in sheep in the age range of 0-1 year, it was determined to be seen by 17.8% in sheep over the age of 2 years. Coenurus cysts, 32.1% were in the right hemisphere of the brain, 46.8% were in the left hemisphere, 12.2% were in both hemispheres, 5.1% were in the cerebellum and 3.8% were in the occipital region. It was a statistically significant finding that the incidence of the cysts in both hemispheres was found only in males (female: 0 cysts, male: 19 cysts) (P<0.05). Coenurus cysts was seen the most frequently in November (10.4%), while it was seen the least frequently in May (2.1%). In the sheep with coenurosis Enolase levels in the blood serum of the total of 80 (40 females, 40 males) animals that were found in the range of 5.44–84.96μg/l. Enolase enzym levels were found to be high in all animals with clinical symptoms. In patients with no symptoms, the results were found to be within the Cut-off limits. The results were statistically significant (P<0.05). Moreover, there was no significant difference between the males and the females. As a result, it was concluded that the enolase enzyme can be used as an auxiliary parameter in the diagnosis of sheep with clinical signs of coenurosis.

Anahtar Kelimeler

Coenurus cerebralis, NSE, Prevalence, Sheep, Taenia multiceps

Kaynakça

• 1. Eckert J., Friedhoff KT., Zahner H., Deplazes P., 2005. Lehrbuch der Parasitologie fu¨r die Tiermedizin. 2th ed., 203-231, Enke Verlag, Stuttgart, Germany. 2. Gashe M., Sewalem M., 2017. Review on cerebral coenurosis in small ruminants. APG, 8, 130-138. 3. Kheirandish R., Sami M., Azizi S., Mirzaei M., 2012. Prevalence, predilection sites and pathological findings of Taenia multiceps coenuri in slaughtered goats from south-east Iran. Onderstepoort J Vet Res, 79,1-5. 4. Yılmaz R., Özyıldız Z., Yumuşak N., 2014. Koyunlarda coenurus cerebralis’in patomorfolojik bulguları. Harran Üniv Vet Fak Derg, 3, 73-77. 5. Schineder T., 2006. Zönurose. In “Veterinerinarmedizinische Parasitologie” Ed., J Boch, C Bauer, 6 ed., 188-190, vollstanding überarbeitete und erweiterte Auflage, Parey, Germany. 6. Sharma DK., Chauhan PPS., 2006. Coenurosis status in Afro-Asian region: Areview. Small Rum Res, 64, 197-202. 7. Uslu U., Güçlü F., 2007. Prevalence of coenurus cerebralis in sheep Turkey. Medycyna Wet, 63,678-680. 8. Achenef M., Markos T., Feseha G., Hibret A., Tembely S., 1999. Coenurus cerebralis infection in Ethiopian Highland Sheep: Incidence and observations on pathogenesis and clinical signs. Trop Anim Health Prod, 31, 15-24. 9. Tavassoli M., Malekifard F., Soleimanzadeh A., Tajik H., 2011. Prevalence of coenurus cerebralis in sheep in Northwest of Iran. Vet Res Forum, 2, 274-276. 10. Soundararajan C., Sivakumar T., Balachandran C., 2017. Coenurus cerebralis and its pathology in an organized farm of Tamil Nadu. J Parasit Dis, 41, 510-513. 11. Abo-Shehada MN., Jebreen E., Arab B., Mukbel R., Torgerson PR., 2002. Prevalence of taenia multiceps in sheep in northern Jordan. Prevent Vet Med, 55, 201-207. 12. Gıcık Y., Kara M., Arslan MÖ., 2007. Prevalence of coenurus cerebralis in sheep in Kars province, Turkey. Bull Vet Inst Pulawy, 51, 379-382. 13. Gökpinar S., Yildiz K., 2012. Prevelance of coenurosis in clinically healthy sheep. Kafkas Univ Vet Fak Derg, 18, A187-A191. 14. Desouky EA., Badawy AI., Refaat RA., 2011. Survey on coenurosis in sheep and goats in Egypt. Vet Ital, 47, 333-340. 15. Bock E., Dissing J., 1975. Determination of enolase activity connected to the 7 brain specific protein 14-3-2. Scand J Immunol, 4, 31-36. 16. Marangos PJ., Schmechel DE., 1987. Neuron specific enolase, a clinically useful marker for neurons and neuroendocrie cells. Annu Rev Neurosci, 10, 269-295. 17. Pancholi V., 2001. Multifunctional alpha-enolase: its role in diseases. Cell Mol Life Sci, 58, 902-920. 18. Lima JE., Takayanagui OM., Garcia LV., Leite JP., 2004. Use of neuron-specific enolase for assessing the severity and outcome in patients with neurological disorders. Braz J Med Biol Res, 37, 19-26. 19. Haque A., Ray SK., Cox A., Banik NL., 2016. Neuron specific enolase: a promising therapeutic target in acute spinal cord injury. Metabolic Brain Dis, 31, 487-495. 20. Kadirov NT., 1959. The epizootiology of moniesiasis and coenuriasis of sheep in the Akmolinsk region. Veterinariya, 36, 30-32. 21. Bondareva VI., Boev SN., Sokolova IB., 1960. Comparative susceptibility of domestic and wild animals to Coenurus infectian. Helminthologia, 2, 224-234. 22. Tiğin Y., 1970. Multiceps multiceps Leske, 1780 (Hall, 1910) biyolojisi ve morfolojisi. Ankara Üniv Vet Fak Derg, 17, 114-135. 23. Verster A., Tustin RC., 1982. Treatment of the larval stage of Taenia multiceps with praziquantel. J S Afr Vet Assoc, 53, 107-108. 24. Ghazaei C., 2005. Evaluation of the effect of antihelminthic agents albendazole, fenbendazole and praziquantel in treatment of coenurosis disease in sheep. J Anim Vet Adv, 4, 852-854. 25. Bıyıkoğlu G., Doğanay A., 1998. Effects of praziquantel and albendazole on coenurus cerebralis in experimentally infected lambs. Turk J Vet Anim Sci, 22, 43-48.