Prepubertal ve Postpubertal Sıçanların Genital Sistem Organlarında Ürotensin 2 Reseptörünün Karşılaştırılması

Yıl 2020, Cilt: 15 Sayı: 2, 113 - 121, 27.10.2020

Duygu Köse , Zekai Halıcı , Erdem Toktay

https://doi.org/10.17094/ataunivbd.666100

Öz

Çalışmada sıçanlarda genital organlarda (over, uterus ve testis) puberte öncesi ve sonrasında Ürotensin 2 Reseptör’ü (ÜTR 2) varlığını ve dağılımını karşılaştırmalı olarak araştırmayı amaçladık. Çalışma toplam 20 adet Wistar Albino cinsi sıçan ile yapıldı. Sıçanlar 2 grup (N:5) erkek (Grup 1: prepubertal erkek, Grup 2: postpubertal erkek) ve 2 grup (N:5) dişi (Grup 3: prepubertal dişi, Grup 4: postpubertal dişi) olmak üzere 4 gruba ayrıldı. Sıçanlardan uterus, over ve testis örnekleri alındı. Moleküler çalışmada ÜTR 2 mRNA ekspresyon seviyeleri gerçek zamanlı polimeraz zincir reaksiyonu) ile incelendi ve immünohistokimyasal olarak ÜTR 2 boyaması ile sonuçlar karşılaştırıp bu dokularda ÜTR 2 varlığı ve dağılımı araştırıldı. Dişi sıçanlarda prepubertal ve postpubertal over ve uterus dokularında ÜTR 2 mRNA ekspresyon seviyeleri değerlendirildiğinde postpubertede istatiksel olarak anlamlı oranda artmış olarak bulunurken, erkek sıçanların testis dokularındaki ÜTR 2 mRNA ekspresyon seviyelerinde prepuberte ve postpubertede arasında bir fark görülmedi. Postpubertal over ve uterusta artan ÜTR 2 seviyelerinin artan östrojen ya da progesteron seviyeleri ile ilişkili olabileceği düşünüldü. Over ve uterus hastalıklarında ÜTR 2’ ünün ayrıntılı olarak araştırılması ve ÜTR 2’ü agonist ve antagonistleri ile ilgili over ve uterus hastalıklarında ileri araştırmalar yapılması gerekliliği sonucuna varıldı.

Anahtar Kelimeler

Over, Testis, Uterus, Ürotensin 2

Destekleyen Kurum

ATATÜRK ÜNİVERSİTESİ BAP BİRİMİ

Proje Numarası

THD-2018-6859

A Comparison of Urotensin 2 Receptor in Genital System Organs of Prepubertal and Postpubertal Rats

Öz

In this study, we aimed to investigate the presence and distribution of urotensin 2 receptor (UTR 2) in the genital organs (ovary, uterus and testis) before and after puberty as prepubertal and postpubertal. A total of 20 Wistar Albino rats were used. The rats were divided into two groups (N: 5) male (Group 1: prepubertal male, Group 2: postpubertal male) and 2 groups (N: 5) female (Group 3: prepubertal female, Group 4: postpubertal female). Uterine, ovarian and testicular samples were collected from rats. In the molecular study, UTR 2 mRNA expression levels were examined by real-time polymerase chain reaction (Real Time-Polymerase Chain Reaction) and immunohistochemical results were compared with the results of UTR 2 staining. UTR 2 mRNA expression levels in the prepubertal and postpubertal ovary and uterine tissues of female rats were found to be significantly increased in postpubertal, but no difference was seen in UTR 2 mRNA expression levels in testicular tissues of male rats. It was thought that increased UTR 2 levels in the postpubertal ovary and uterus may be associated with increased estrogen or progesterone levels. It was concluded that UTR 2 should be investigated in detail in ovarian and uterine diseases and further investigations of ovarian and uterine diseases related to UTR 2 agonists and antagonists should be performed.

Anahtar Kelimeler

Overy, Testis, Uterus, Urotensin 2

Proje Numarası

THD-2018-6859

Kaynakça

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