Wistar Albino Sıçanlarda Michauxia campanuloides L’Hér.’in Bazı Sperma Parametreleri Üzerine Etkisi

Yıl 2020, Cilt: 15 Sayı: 2, 138 - 144, 27.10.2020

Recep Hakkı Koca , Muhammed Mesud Hürkul , Serdal Kurt , Ayşegül Köroğlu

https://doi.org/10.17094/ataunivbd.700491

Öz

Bu çalışmanın amacı sıçanlarda Michauxia campanuloides L’Hér. kullanımının bazı sperma parametreleri üzerine etkisini araştırmaktır. Sunulan çalışmanın bitki materyali antioksidan özellikli Michauxia campanuloides L’Hér.'dir. Çalışmanın hayvan materyalini 18 adet erkek Wistar albino sıçan oluşturmuştur. Sıçanlar kontrol (K; n=6), uygulama 1 (U1; n=6) ve uygulama 2 (U2; n=6) olmak üzere üç gruba ayrılmış ve M. campanuloides L’Hér.'in liyofilize sulu ekstresi U1 grubundaki sıçanlara 20 mg/kg ve U2 grubundaki sıçanlara 40 mg/kg dozunda 21 gün süreyle oral yolla günlük uygulandı. Çalışma sonunda tüm sıçanlar kan serumu total antioksidan kapasitesi (TAK), sperma yoğunluğu, sperma motilitesi, anormal spermatozoon oranı ile testis, epididimis, seminal bez ve ventral prostat ağırlığı yönünden muayene edildi. Sperma yoğunluğu K ve U1 gruplarına göre U2 grubunda anlamlı düzeyde daha yüksek bulundu (P=0,002). Anormal spermatozoon oranının K grubuna göre U1 ve U2 grubunda anlamlı düzeyde daha düşük olduğu tespit edildi (P=0,003). Seminal bez ağırlığı K grubuna göre U1 ve U2 gruplarında istatistiksel olarak daha yüksek ölçüldü (P=0,016). Sonuç olarak, M. campanuloides ekstresinin oral tüketimi sıçanlarda sperma kalitesinde bir artış sağlamaktadır.

Anahtar Kelimeler

Antioksidan, Michauxia campanuloides, Sıçan, Sperma parametreleri

The effect of Michauxia campanuloides L’Hér. on Some Semen Parameters in Wistar Albino Rats

Öz

The aim of this study was to investigate the effect of Michauxia campanuloides L’Hér. on some semen parameters in rats. The plant material of the study is Michauxia campanuloides L'Hér. having antioxidant property. The animal material of the study consisted of 18 male Wistar albino rats. The rats were divided into three groups as control (C; n=6), treatment 1 (T1; n=6) and treatment 2 (T2; n=6). Lyophilized aqueous extract of M. campanuloides L’Hér. was administered orally at a dose of 20 mg/kg/day for rats in the T1 group and 40 mg/kg/day for rats in the T2 group for 21 days. At the end of the study, all rats were examined for blood total antioxidant capacity (TAC), semen density, semen motility, abnormal spermatozoon ratio along with testicular, epididymal, seminal gland and ventral prostate weight. Semen density was significantly higher in the T2 group compared to the C and T1 groups (P=0.002). Rates of abnormal spermatozoa were significantly lower in T1 and T2 groups than the C group (P=0.003). The weight of seminal gland was significantly higher in the T1 and T2 groups compared to the C group (P=0.016). In conclusion, oral consumption of M. campanuloides extract provides an increase in semen quality in rats.

Anahtar Kelimeler

Antioxidant, Michauxia campanuloides, Rat, Semen parameters

Kaynakça

• 1. Khaki A., Fathı AF., Ahmadı AHR., Rastgar H., Rezazadeh SA., 2009. Effects of danae racemosa on spermatogenesis in Rat. J Med Plants, 3, 87- 92.
• 2. Rizzo A., Roscino MT., Binetti F., Sciorsci RL., 2012. Roles of reactive oxygen species in female reproduction. Reprod Domest Anim, 47, 344- 352.
• 3. Sukhotnik I., Nativ O., Ben-Shahar Y., Bejar IN., Pollak Y., Coran AG., Gorenberg M., 2019. Antioxidant treatment ameliorates germ cell apoptosis induced by a high-dose ionizing irradiation in rats. Pediatr Surg Int, 35, 137-143.
• 4. Aitken RJ., 2020. Impact of oxidative stress on male and female germ cells: implications for fertility. Reproduction, 159, 189-201.
• 5. Walczak–Jedrzejowska R., Wolski JK., Slowikowska–Hilczer J., 2013. The role of oxidative stress and antioxidants in male fertility. Cent European J Urol, 66, 60-67.
• 6. Benabbou A., Khaled MB., Alchalabi AS., 2018. Evaluation of the efficiency of combined and separated antioxidant supplementation of vitamin C and E on semen parameters in streptozotocin- induced diabetic male Wistar rats. SAJEB, 7, 166-72.
• 7. Zhang K., Fu L., An Q., Hu W., Liu J., Tang X., Gu Y., 2020. Effects of Qilin pills on spermatogenesis, reproductive hormones, oxidative stress, and the TSSK2 gene in a rat model of oligoasthenospermia. BMC Comp Med Ther, 20, 1-11.
• 8. Aitken RJ., Roman SD., 2008. Antioxidant systems and oxidative stress in the testes. Omcl, 1, 15-24.
• 9. Javadi L., Farzadi L., Fathiazad F., Nouri M., 2011. Anti-oxidative effects of citro flavonoids on spermatogenesis in rat. Afr J Pharm Pharmacol, 5, 721-725.
• 10. Neha K., Haider MR., Pathak A., Yar MS., 2019. Medicinal prospects of antioxidants: A review. Eur J Med Chem, 178, 687-704.
• 11. Mironczuk-Chodakowska I., Witkowska AM., Zujko ME., 2018. Endogenous non-enzymatic antioxidants in the human body. Adv med sci, 63, 68-78.
• 12. Sen S., Chakraborty R., 2011. The role of antioxidants in human health. Oxidative stress: diagnostics, prevention and therapy. ACS Publications, 1, 1-37.
• 13. Sen S., Chakraborty R., Sridhar C., Reddy YSR., De B., 2010. Free radicals, antioxidants, diseases and phytomedicines: current status and future prospect. Int J Pharm Sci Rev Res, 3, 91-100.
• 14. Hürkul MM., Köroğlu A., 2019. Campanulaceae familyasının etnobotanik kullanımı, kimyasal içeriği ve biyolojik aktivitesi üzerine bir derleme. Türk Far. Derg, 4, 70-84.
• 15. Güvenç A., Akkol EK., Hürkul MM., Süntar İ., Keleş H., 2012. Wound healing and antiinflammatory activities of the Michauxia L’Hérit (Campanulaceae) species native to Turkey. J Ethnopharmacol, 139 401-408.
• 16. Yokoi K., Uthus EO., Nielsen FH., 2003. Nickel deficiency diminishes sperm quantity and movement in rats. Biol Trace Elem Res, 93, 141- 153.
• 17. Bearden HJ., Fuquay JW., 2000. Semen evaluation. In “Applied Animal Reproduction”, Ed., HJ Bearden, JW Fuquay, ed., 168-182, Prentice Hall, New Jersey.
• 18. Tejidos SECE., 2007. Toxic effects of methanolic extract of Aspilia africana leaf on the estrous cycle and uterine tissues of Wistar rats. Int J Morphol, 25, 609-614.
• 19. Sadat SS., Mohammadi S., Sazegar G., Fazel A., Ebrahimzadeh A., Mobarhan MG., Tavallaei S., 2019. Effects of carob fruit extract on spermatogenesis, antioxidant status, and apoptosis in adult male mice. Pharm Sci, 25, 184- 189.
• 20. Asif M., 2015. Chemistry and antioxidant activity of plants containing some phenolic compounds. Chem Int, 1, 35-52.
• 21. Zhong RZ., Zhou DW., 2013. Oxidative stress and role of natural plant derived antioxidants in animal reproduction. J Integr Agric, 12, 1826- 1838.
• 22. Kaurinovic B., Vastag D., 2019. Flavonoids and phenolic acids as potential natural antioxidants. In “Antioxidants”, Ed., E Shalaby, 2-20, Intech Open, London.
• 23. Razavi SR., Khadivi F., Hashemi F., Bakhtiari A., 2019. Effect of zinc on spermatogenesis and sperm chromatin condensation in bleomycin, etoposide, cisplatin treated rats. Cell Journal, 20, 521-526.
• 24. Khaki A., Ouladsahebmadarek E., Javadi L., Farzadi L., Fathiazad F., Nouri M., 2011. Antioxidative effects of citro flavonoids on spermatogenesis in rat. Afr J Pharm, 5, 721-725.
• 25. Amin A., Hamza AA., 2006. Effects of Roselle and Ginger on cisplatin-induced reproductive toxicity in rats. AJA, 8, 607-612.
• 26. Zubkova EV., Robaire B., 2004. Effect of glutathione depletion on antioxidant enzymes in the epididymis, seminal vesicles, and liver and on spermatozoa motility in the aging brown Norway rat. Biol reprod, 71, 1002-1008.
• 27. Patil RB., Vora SR., Pillai MM., 2012. Protective effect of Spermatogenic activity of Withania somnifera (Ashwagandha) in galactose stressed mice. Ann Biol Res, 3, 4159-4165.
• 28. Gonzales GF., Vasquez VB., Gasco M., 2013. The transillumination technique as a method for the assessment of spermatogenesis using medicinal plants: the effect of extracts of black maca (Lepidium meyenii) and camu camu (Myrciaria dubia) on stages of the spermatogenic cycle in male rats. Toxicol Mech Methods, 23, 559-565.