Kedi ve Köpeklerden İzole Edilen Staphylococcus Türlerinde Çoklu İlaç Dirençliliğinin Araştırılması

Yıl 2020, Cilt: 15 Sayı: 2, 156 - 166, 27.10.2020

Hüban Göçmen , Hazel Tamakan , Halit Şükür , Ömer Memduh Esendal

https://doi.org/10.17094/ataunivbd.706993

Öz

Kedi ve köpeklerden sıklıkla izole edilen Staphylococcus türlerinin metisilin ve çoklu ilaç dirençliliği (MDR) artışı çalışmamızın temelini oluşturdu. Bu amaçla, Yakın Doğu Üniversitesi Hayvan Hastanesi’ne getirilen 100 adet köpek ve 42 adet kediye ait toplamda 142 adet klinik örnek (yara, kulak, göz, burun, deri svapları gibi) çalışıldı. İncelenen örneklerden izole edilen Staphylococcus spp. izolatlarının tür düzeyinde identifikasyonu, VITEK-2 mikrobiyal ID/ADT test sistemi ve API Staph identifikasyon sistemi ile gerçekleştirildi. Staphylococcus izolatlarının antimikrobiyal duyarlılık analizleri VITEK-2 otomatize sistem aracılığı ile gerçekleştirildi. İncelenen numunelerden 78 adet (%54.92) Staphylococcus spp. izole edildi. İzolatların 52’si (%66.67) Koagulaz pozitif Staphylococcus (CoPS) ve 16’sı (%20.51) Koagulaz negatif Staphylococcus (CoNS) olarak identifiye edildi. CoPS’lar arasında en yüksek oranda identifiye edilen türlerin Staphylococcus (S.) pseudintermedius (%38,46) ve S. aureus (%25.64); CoNS’lar arasında ise S. chromogenes (%10.26) olduğu belirlendi. Antimikrobiyal madde duyarlılık analizleri sonucu, 11 (%28.2) izolat metisilin dirençli S. aureus (MRSA), 10 (%25.64) izolat metisilin dirençli S. pseudintermedius (MRSP) ve 10 (%25.64) izolat metisilin dirençli Koagulaz negatif Staphylococcus (MRCoNS) olarak belirlendi. Ayrıca çoklu ilaç direnci tespit edilen türlerin 6’sında (%40.00) MDR-MRSP ve 4’ünde (%26.66) MDR-MRSA pozitifliği belirlendi. Toplamda 23 adet MDR Staphylococcus spp. izolatının 21 adedi köpeklerden ve 2 adedi de kedilerden izole edilmiştir. Çalışmada elde edilen veriler ışığında artan çoklu ilaç direncinin güncel yaklaşımlarını hayvanlardan izole edilen Staphylococcus türlerinde inceleyerek tedavi protokollerinin bu çerçevede uygulanmasına katkı sağlandı.

Anahtar Kelimeler

Kedi, Köpek, Stafilokok türleri, Metisilin dirençliliği, Çoklu ilaç direnci

Investigation of Multidrug Resistance among Staphylococcus Species Isolated from Cats and Dogs

Öz

The increase in methicillin and multidrug resistance (MDR) of Staphylococcus species, which are frequently isolated from cats and dogs, formed the basis of our study. For this purpose, a total of 142 clinical samples (wound, ear, eye, nasal, skin swabs, etc.) belonging to 100 dogs and 42 cats brought to the Near East University Animal Hospital were studied in our investigation. Species-level identification of Staphylococcus spp. isolated from the samples examined was performed with the VITEK-2 microbial ID/AST test system and API Staph identification system. Antimicrobial susceptibility analyzes of Staphylococcus isolates were carried out through the VITEK-2 automated system. From the samples, 78 (54.92%) Staphylococcus spp. were isolated. 52 (66.67%) of the isolates were identified as Coagulase positive Staphylococcus (CoPS) and 16 (20.51%) as Coagulase negative Staphylococcus (CoNS). Among CoPS, the most frequently identified species were S. pseudintermedius (38.46%) and S. aureus (25.64%); whereas among CoNS, S. chromogenes (10.26%) was determined as the most predominant. According to the results of antimicrobial susceptibility analyzes, 11 (28.2%) isolates were determined as methicillin resistant S. aureus (MRSA), 10 (25.64%) as methicillin resistant S. pseudintermedius (MRSP) and 10 (25.64%) isolate methicillin resistant Coagulase negative Staphylococcus (MRCoNS). Besides, 6 (40.00%) MDR-MRSP and 4 (26.66%) MDRMRSA positivity were determined among the species with multiple drug resistance. Of the 23 MDR Staphylococcus spp. isolates in total, 21 were isolated from dogs and 2 from cats. By analyzing the current approaches of the increased drug resistance with the data obtained in this study in Staphylococcus species isolated from animals, necessary contributions have been implemented to the treatment protocols within this framework.

Anahtar Kelimeler

Cat, Dog, Methicillin resistance, Multidrug resistant, Staphylococcus resistance

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