İneklerde Östrus Siklusu Süresince Uterus Ekojenitesindeki Değişikliklerin Araştırılması

Yıl 2020, Cilt: 15 Sayı: 3, 263 - 270, 31.12.2020

Lokman Şaki Bülent Polat

https://doi.org/10.17094/ataunivbd.756769

Cited By: 1

Öz

Sunulan çalışmanın amacı ineklerde östrus siklusunun farklı günlerinde elde edilen ekotekstür parametreleri kullanılarak uterusta meydana gelebilecek olası değişikliklerin incelenmesidir. Çalışmada Erzurum’da özel bir işletmede bulunan, klinik veya subklinik endometritis belirtisi gözlenmeyen, 1. laktasyondaki 42 adet inek kullanıldı. Östrus gözlenen ineklerde östrus günü (0. gün), siklusun farklı günlerinde (4, 12, 16, 18, 21. günler) ultrasonografik görüntüler alındı. Uterus endometriyumundan alınan görüntülerdeki ortalama gri değer (MGL), homojenite (HOM) ve kontrast (CON) parametreleri bilgisayar destekli görüntü analiz programıyla incelendi. İneklerde östrus siklusunun farklı günlerindeki ortalama ekotekstür parametreleri; MGL için 68.43±0.55, HOM için 0.080±0.001, ve CON için 54.23±0.37 olarak bulundu. Ortalama gri değer ile HOM arasında negatif, CON değerleriyle ise pozitif bir ilişki olduğu saptanırken, HOM ve CON değerleri arasında da ise negatif bir ilişki olduğu gözlemlendi. Sonuç olarak siklusun farklı dönemlerindeki ineklerin endometriyumundan alınan görüntülerdeki MGL, HOM ve CON değerleri arasında istatistiksel fark saptanmadı. Siklus folliküler ve luteal dönem olarak değerlendirildiğinde ise; MGL değerlerinin siklusun folliküler fazında yüksek, luteal fazında ise düşük seyrettiği homojenite değerlerinin ise foliküler fazda düşük, luteal fazda ise yüksek seyrettiği görüldü.

Anahtar Kelimeler

Ekotekstür, endometriyum, inek, Östrus siklusu

Destekleyen Kurum

Atatürk Üniversitesi BAP Koordinasyon Birimi

Proje Numarası

PRJ2015/293, PRJ2015/294

Investigation of Changes in The Uterine Echogenicity of Cows During Oestrus Cycle in Cows

Öz

The aim of the present study is to examine possible changes that may occur in the uterus by using the echotexture parameters obtained on different days of the oestrus cycle in cows. In the study, 42 cows in the first lactation, with no clinical or subclinical endometritis symptoms, in a private farm in Erzurum were used. Ultrasonographic images were taken on the oestrus day (0thday) and on different days of the cycle (4, 12, 16, 18, 21) in cows with oestrus. The mean gray value (MGL), homogeneity (HOM), and contrast (CON) parameters in the images taken from the uterine endometrium were examined with a computer-assisted image analysis program. Average echotexture parameters on the different days of the estrus cycle in cows were detected as 68.43±0.55 for MGL, 0.080±0.001 for HOM, and 54.23±0.37 for CON. There was a negative relationship between the mean gray value and HOM, and a positive relationship with the CON values, while a negative relationship was observed between the HOM and CON values. As a result, no statistical difference was found between MGL, HOM and CON values in the images taken from the endometrium of cows in different periods of the cycle. When the cycle is evaluated as follicular and luteal period; It was observed that MGL values were high in the follicular phase of the cycle and low in the luteal phase, while homogeneity values were low in the follicular phase and high in the luteal phase.

Anahtar Kelimeler

Cow, Echotexture, Endometrium, Oestrus cycle

Proje Numarası

PRJ2015/293, PRJ2015/294

Kaynakça

• 1. Forde N., Beltman M., Lonergan P., Diskin M., Roche J., Crowe M., 2011. Oestrous cycles in Bos taurus cattle. Anim Reprod Sci, 124, 163-169. 2. Johnston D., Estepa IM., Ebhardt H., Crowe M., Diskin M., 2018. Differences in the bovine milk whey proteome between early pregnancy and the estrous cycle. Theriogenology, 114, 301-307. 3. Camela ES., Nociti RP., Santos VJ., Macente BI., Murawski M., Vicente WR., Bartlewski PM., Oliveira MEF., 2019. Changes in testicular size, echotexture, and arterial blood flow associated with the attainment of puberty in Dorper rams raised in a subtropical climate. Reprod Dom Anim, 54, 131-137. 4. Hasbi H., Gustina S., 2020. Comparative of monitoring estrus cycle in livestock: Hormonal features and ultrasound. J Ilmu-Ilmu Peternakan, 30, 10-18. 5. Kaymaz M., Fındık M., Rişvanlı A., Köker A., 2019. Çiftlik Hayvanlarında Doğum ve Jinekoloji. 3th ed. 47-48, Medipress, Malatya-Türkiye. 6. Senger P., 2005. Pathways to pregnancy and parturition. 2nd ed. Ames: Current Concepts. 7. Toledo-Alvarado H., Vazquez AI., de los Campos G., Tempelman RJ., Gabai G., Cecchinato A., Bittante G., 2018. Changes in milk characteristics and fatty acid profile during the estrous cycle in dairy cows. J Dairy Sci, 101, 9135-9153. 8. Ball PJ, Peters AR. 2008. Reproduction in cattle 3th ed. Blackwell Publishing. 9. Morris D., Diskin M., 2008. Effect of progesterone on embryo survival. Animal, 2, 1112-1119. 10. Wang C., Robinson R., Flint A., Mann G., 2007. Quantitative analysis of changes in endometrial gland morphology during the bovine oestrous cycle and their association with progesterone levels. Reproduction, 134, 365-371. 11. Hermes R., Hildebrandt TB., Goritz F., Jewgenow K., Lengwinat T., Hofmann RR., 2000. Ultrasonography of the ovaries and uterus and grey scale analysis of the endometrium during embryonic diapause in European roe deer. Acta Theriol, 45, 559-572. 12. Schmauder S., Weber F., Kiossis E., Bollwein H., 2008. Cyclic changes in endometrial echotexture of cows using a computer-assisted program for the analysis of first-and second-order grey level statistics of B-Mode ultrasound images. Anim Reprod Sci, 106, 153-161. 13. Fiore E., Perillo L., Morgante M., Giudice E., 2018. Ultrasonographic measuement of liver, portal vein, hepatic vein and perivisceral adipose tissue in high-yielding dairy cows with fatty liver during the transition period. J Dairy Res, 85, 431-438. 14. Fabbri G., Gianesella M., Morgante M., Armato L., Bonato O., Fiore E., 2020. Ultrasonographic alterations of bovine claws sole soft tissues associated with claw horn disruption lesions, body condition score and locomotion score in Holstein dairy cows. Res Vet Sci, 131, 146-152. 15. Cengiz M., Kanca H., Salar S., Bastan A., Kucukaslan I., Alkan H., Karakas K., Yuksel O., Hayırlı H., 2014. Endometrial echotexture parameters in Turkish Saanen Goats (Akkeci) during oestrus and early pregnancy. Anim Reprod Sci, 146, 27-33. 16. Kauffold J., von dem Bussche B., Failing K., Wehrend A., Wendt M., 2010. Use of B-mode ultrasound and grey-scale analysis to study uterine echogenicity in the pig. J Reprod Dev, 56, 444-448. 17. Ginther O., 2014. How ultrasound technologies have expanded and revolutionized research in reproduction in large animals. Theriogenology, 81, 112-125. 18. Cengiz M., Çolak A., Hayırlı A., Cannazik O., 2017. Optical density changes in ultrasonographic images of the endometrium and corpus luteum in pregnant and cyclic cows. Turk J Vet Anim Sci, 41, 18-24. 19. Scully S., Evans A., Carter F., Duffy P., Lonergan P., Crowe M., 2015. Ultrasound monitoring of blood flow and echotexture of the corpus luteum and uterus during early pregnancy of beef heifers. Theriogenology, 83, 449-458. 20. Erdoğan G., Küçük N., Kanca H., Aksoy M., 2017. In vivo and in vitro assessment of ovarian echotexture through computer assisted real time ultrasonography in bitches. Ankara Üniv Vet Fak Derg, 64, 171-176. 21. Küçükaslan I., Kaya D., Emre B., Bollwein H., Özyurtlu N., Mülazımoğlu S., Aslan S., 2014. Evaluation of endometrial echotexture and cervical cytology in cows during and after treatment of endometritis. Tierarztl Prax Ausg G Grosstiere Nutztiere, 42, 343-350. 22. Tom J., Pierson R., Adams G., 1998. Quantitative echotexture analysis of bovine corpora lutea. Theriogenology, 49, 1345-1352. 23. Lefebvre F., Meunier M., Thibault F., Laugier P., Berger G., 2000. Computerized ultrasound B-scan characterization of breast nodules. Ultrasound Med Biol, 26, 1421-1428. 24. Kasimanickam R., Duffield T., Foster R., Gartley C., Leslie K., Walton J., Johnson WH., 2004. Endometrial cytology and ultrasonography for the detection of subclinical endometritis in postpartum dairy cows. Theriogenology, 62, 9-23. 25. Sheldon IM., Lewis GS., LeBlanc S., Gilbert RO., 2006. Defining postpartum uterine disease in cattle. Theriogenology, 65, 1516-1530. 26. Bonafos L., Kot K., Ginther O., 1995. Physical characteristics of the uterus during the bovine estrous cycle and early pregnancy. Theriogenology, 43, 713-721. 27. Kastelic J., Curran S., Ginther O., 1989. Accuracy of ultrasonography for pregnancy diagnosis on days 10 to 22 in heifers. Theriogenology, 31, 813-820. 28. Mihandoost B., Mogheiseh A., Nazifi S., Ahmadi MR., Ansari-Lari M., 2019. Metabolic status and ultrasound traits of reproductive tract of estrus dairy cows in early postpartum period. Comparative Clin Path, 28, 689-693. 29. Zonturlu AK., Akkus T., Kacar C., Korkmaz O., Semra K., Demir MC., Emre B., 2018. Discrimination of early pregnancy and endometrial cyst by ultrasonographic assessment of uterine echotexture in Mares. Kafkas Univ Vet Fak Derg, 24, 519-524. 30. Spencer TE., Bazer FW., 2004. Conceptus signals for establishment and maintenance of pregnancy. Reprod Biol End, 2, 1-15. 31. Ohtani S., Okuda K., Nishimura K., Mohri S., 1993. Histological changes in bovine endometrium during the estrous cycle. Theriogenology, 39, 1033-1042