Anaplazmozisli Koyunlarda Bazı Inflamasyon Markırlarının Araştırılması

Yıl 2020, Cilt: 15 Sayı: 3, 257 - 262, 31.12.2020

Gönül Bayram Leyla Mis Ali Cınar Bekir Oğuz

https://doi.org/10.17094/ataunivbd.787185

Öz

Anaplazmozis koyunlarda sıklıkla görülen, kan hücrelerini enfekte eden artropod kaynaklı ve ekonomik kayıplara neden olabilen paraziter bir hastalıktır. Bu çalışma Anaplazmozis ile enfekte koyunlarda bazı serum inflamasyon markır seviyelerini değerlendirmek için planlanmıştır. Yapılan araştırma için kullanılacak olan kan örnekleri, Van Büyükşehir Belediyesi Mezbahanesi’ne getirilen 20 sağlıklı ve 20 Anaplazmozis’li koyundan sağlandı. Kontrol ve hasta gruplarının belirlenmesi, hastalığın klinik belirtileri, giemsa boyalı kan frotileri ve serolojik yöntem (cELISA) yardımıyla yapıldı. Toplanan 91 koyunların ait serum örneği Anaplazma spp. türlerinin antikorlarının varlığı yönünde serolojik olarak incelendiğinde %73.6’sı (67/91) Anaplazmozis yönünden seropozitif bulundu. Kan frotisinde Anaplazma etkenleri görünen ve ELISA inhibisyon değeri yüksek çıkan koyunların kanları analizler için kullanıldı. Anaplazmozis’li koyunlar teşhis edildikten sonra pozitif ve negatif olarak gruplandırıldı. Pozitifliği tespit edilen 20 koyun serumunda granülosit makrofaj koloni uyarıcı faktör (GM-CSF), interlöykin-1β (IL-1β), interlöykin-6 (IL-6), tumor nekroz faktör- alfa (TNF-α) düzeyleri elisa kiti ile kit prosedürüne göre belirlendi. Proinflamasyon parametrelerinden serum IL-1β (ng/L), TNF-α (ng/L), GM-CSF (ng/L) düzeylerinin negatif gruba göre istatiksel olarak yükseldiği tespit edildi. Anaplazmaya karşı gelişen inflamasyon ile ilgili mekanizmaların incelenmesi, ileride geliştirilecek immunoterapi araştırmaları için Anaplazma enfeksiyonlarının teşhis ve önlemeye yönelik çalışmalara katkı sağlayacağı düşünülmektedir.

Anahtar Kelimeler

Anaplazmosis, GM-CSF, interlökin-1, interlökin-6, TNF-alfa

Destekleyen Kurum

Yüzüncü Yıl Üniversitesi Bilimsel Araştırma Projeleri Başkanlığı

Proje Numarası

TYL-2019-8141

Investigation of Some Markers of Inflammation in Sheep with Anaplasmosis

Öz

Anaplasmosis is an arthropod-borne parasitic disease that infects blood cells and frequently seen in sheep. This study was planned to evaluate inflammation marker levels in sheep infected with anaplasmosis. In this research, the blood samples used were obtained from 20 healthy and 20 Anaplasmosis sheep brought to Van Metropolitan Municipality Slaughterhouse. Determination of control and patient groups, clinical symptoms of the disease, Giemsa stained blood smears and serological method (cELISA) were used. Serum samples of 91 sheep collected from Anaplasma spp. When examined serologically for the presence of antibodies of the species, 73.6% (67/91) were found to be seropositive in terms of Anaplasmosis. The blood of the sheep with high ELISA inhibition values and showing Anaplasma morulae form in the peripheral blood smears, were used for analysis. Granulocyte macrophage colony stimulating factor (GM-CSF), interleukin-1β (IL-1β), interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α) levels in 20 sheep whose positivity was detected were identified with the Elisa kit. IL-1β (ng/L), TNF-α (ng/L), GM-CSF (ng/L) levels from among the pro-inflammation parameters were found to increase. It is thought that researching the mechanisms related to inflammation developing against Anaplasma will contribute to studies for diagnosis and prevention of Anaplasma infections for future immunotherapy research.

Anahtar Kelimeler

Anaplasmosis, GM-CSF, Interleukin-1, Interleukin-6, TNF-alpha

Proje Numarası

TYL-2019-8141

Kaynakça

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