Araştırma Makalesi
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The Use of Grape Seed (Vitis vinifera L.) Extract as Feed Additive Against Negative Effects of Gentamycin in Broiler

Yıl 2020, Cilt: 15 Sayı: 3, 271 - 278, 31.12.2020
https://doi.org/10.17094/ataunivbd.793341

Öz

In this study, it was aimed to determine the effects of V. vinifera L seed extract against the negative effects of performance parameters, organ weights and blood parameters due to the administration of gentamycin in broilers. A total of 240 Ross 308 chickens were divided into 4 groups with 6 replications of 10 birds per cage. Treatment groups: Control group was fed a basal diet only; group V was fed a diet supplemented with grape seed extract (100 mg/kg); group G was fed a basal diet and administered gentamycin (50 mg/kg body weight intramuscularly); group V+G was fed a diet supplemented with grape seed extract, and gentamycin in levels as in group V and G. The highest weight gain and feed intake at the 3rd, 4th, 5th and 6th weeks was observed in group C, and the lowest, statistically significant, was in group G. The highest carcass weight was in group C. The highest aspartate aminotransferase and alanine aminotransferase levels were in group G. The lowest red blood cell value was in group C. Liver weight was increased significantly in group G. In this study, it was observed that the use of gentamycin harmed the health of broiler hens, but these effects could be reduced partially with the grape seed extract added to the feed. For this reason, grape seed extract may be used as a feed additive with healing effect.

Destekleyen Kurum

Ataturk University, Scientific Research Foundation

Proje Numarası

Project Number: 2012/063

Kaynakça

  • 1. Islam NU., Khan MZ., Saleemi MK., Khan A., Bhatti SA., Yousaf M., Hassan ZU., 2011. Clinicopathological studies on gentamycin toxicity in White Leghorn commercial layers. Pak Vet J, 31, 305-308. 2. Akbarian A., Michiels J., Degroote J., Majdeddin M., Golian A., De Smet S., 2016. Association between heat stress and oxidative stress in poultry; mitochondrial dysfunction and dietary interventions with phytochemicals. J Anim Sci Biotechnol, 7, 37. 3. Lobo V., Patil A., Phatak A., Handra N., 2010. Free radicals, antioxidants and functional foods: impact on human health. Pharmacogn Rev, 4, 118-126. 4. Stambuk P., Tomaskovic D., Tomaz I., Maslov L., Stupic D., Karoglan Kontic J., 2016. Application of pectinases for recovery of grape seeds phenolics. 3 Biotech, 6, 224. 5. Cosansu S., Juneja VK., 2018. Growth of Clostridium perfringens in sous vide cooked ground beef with added grape seed extract. Meat Sci, 143, 252-256. 6. Özcan MM., Al Juhaimi F., Gülcü M., Uslu N., Geçgel Ü., 2017. Determination of bioactive compounds and mineral contents of seedless parts and seeds of grapes. S Afr J Enol Vitic, 38, 212-220. 7. Zhang S., Li L., Cui Y., Luo L., Zhou P., Sun B., 2017. Preparative high‐speed counter-current chromatography separation of grape seed proanthocyanidins according to degree of polymerization. Food Chem, 219, 399-407. 8. Kemperman RA., Gross G., Mondot S., Possemiers S., Marzorati M., Wiele TV., Vaughan EE., 2013. Impact of polyphenols from black tea and red wine/grape juice on a gut model microbiome. Food Res Int, 53, 659-669. 9. Charradi K., Mahmoudi M., Bedhiafi T., Jebari K., El May MV., Limam F., Aouani E., 2018. Safety evaluation, anti-oxidative and anti-inflammatory effects of subchronically dietary supplemented high dosing grape seed powder (GSP) to healthy rat. Biomed&Pharmacother, 107, 534-546. 10. Apaydin Yildirim B., Tunc MA., Yildirim S., Yildirim F., Kordali S., Ertekin A., Aktas Senocak E., 2017. The effect of Vitis vinifera L.cv. Merlot seed extract on gentamycin-induced nephrotoxicity in broilers. Europ Poult Sci, 81, 1-12. 11. Jing S., Zhang X., Yan LJ., 2015. Antioxidant activity, antitumor effect, and antiaging property of proanthocyanidins extracted from Kunlun Chrysanthemum flowers. Oxid Med Cell Longev, 2015, 1-10. 12. Kordalı Ş., Çakır A., Akcın TA., Mete E., Akcın A., Aydın T., Kılıç H., 2009. Antifungal and herbicidal properties of essential oils and n-hexane extracts of Achillea gypsicola Hub-Mor. and Achillea biebersteinii Afan. (Asteraceae). Ind Crops Prod, 29, 562-570. 13. SPSS (2004). SPSS for Windows Release 13.0. SPSS Inc. 14. Arafat N., Awadin WF., El-Shafei RA., Farag VME., Saleh RM., 2018. Protective Role of Moringa oleifera Leaves extract against gentamycin -induced nephro- and hepato-toxicity in chickens. AJVS, 58, 173-185. 15. Arivuchelvan A., Murugesan S., Mekala P., 2012. Antioxidant properties of Ocimum sanctum in broilers treated with high doses of gentamycin. IJDAD, 1, 143-146. 16. Khan I., Khan MZ., Saleemı MK., Javed I., Khan A., 2008. Pathological and Biochemical Effects of Intramuscular Gentamycin Administration in Chickens. Turk J Vet Anim Sci, 32, 345-351. 17. Javed U., Saleemi MK., Khan MZ., Khan A., Javed I., 2008. Pathological Effects of Gentamycin in Growing Broilers. Proceedings, The 15th Congress of FAVA. FAVA -OIE Joint Symposium on Emerging Diseases. 27-30 October 2008. Bangkok, Thailand, 17-18. 18. Javed U., Khan MZ., Saleemi MK., Khan A., Javed I., Rafique S., 2013. Toxico pathological effects of parenteral administration of gentamycin in growing Broilers. Int J Agric & Biol Eng, 15, 529-534. 19. Wang ML., Suo X., Gu JH., Zhang WW., Fang Q, Wang X., 2008. Influence of grape seed proanthocyanidin extract in Broiler chickens: Effect on chicken coccidiosis and antioxidant status. Poult Sci, 87, 2273-2280. 20. Abu Hafsa SH., Ibrahim SA., 2018. Effect of dietary polyphenol- rich grape seed on growth performance, antioxidant capacity and ileal microflora in broiler chicks. J Anim Physiol Anim Nutr, 102, 268-275. 21. Ismail AM., Sedki AA., Abdallah AG., 2003. Influence of black seed, garlic and onion supplementation on reproductive performance in rabbits. Egypt J Agric Res, 81, 1193-1207. 22. Kermauner A., Laurencic A., 2008. Supplementation of rabbit diet with chestnut wood extract: Effect on in vitro gas production from two sources of protein. Proceedings of the 9thWorld Rabbit Congress, 10–13 Jun, Verona, Italy, 689-693. 23. Taranu I., Marin DE., Palade M., Pistol GC., Chedea VS., Gras MA., Rotar C., 2019. Assessment of the efficacy of a grape seed waste in counteracting the changes induced by aflatoxin B1 contaminated diet on performance, plasma, liver and intestinal tissues of pigs after weaning. Toxicon, 162, 24-31. 24. Brenes A., Viveros A., Goni I., Centeno C., Sa yago-Ayerdy SG., Arija I., Saura-Calixto F., 2007. Effect of grape pomace concentrate and vitamin E on digestibility of polyphenols and antioxidant activity in chickens. Poult Sci, 87, 307-316. 25. Hajati H., Hassanabadi A., Golian A., Nassiri-Moghaddam H., Nassiri MR., Safari R., 2015. The effect of grape seed extract and vitamin C feed supplements carcass characteristics, gut morphology and ileal microflora in broiler chickens exposed to chronic heat stress. IJAS, 5, 155-165. 26. Erişir Z., Şimşek ÜG., Özçelik M., Baykalır Y., İflazoğlu Mutlu S., Çiftçi M., 2018. Effects of dietary grape seed on performance and some metabolic assessments in Japanese quail with different plumage colors exposed to heat stress. R Bras Zootec, 47, 1-10. 27. Center SA., 2007. Interpretation of liver enzymes. Vet Clin N Am-Small, 37, 297-333. 28. Saleemi MK., Khan MZ., Khan A., Javed I., 2009. Pathological effects of gentamycin administered intramuscularly to day old broiler chicks. Exp Toksicol Pathol, 61, 425-432. 29. Rajput SA., Sun L., Zhang N., Khalil MM., Gao X., Ling Z., Zhu L., Khan FA., Zhang J., Qi D., 2017. Ameliorative effects of grape seed proanthocyanidin extract on growth performance, immune function, antioxidant capacity, biochemical constituents, liver histopathology and aflatoxin residues in Broilers Exposed to Aflatoxin B1. Toxins, 9, 371-391. 30. Gines I., Gil-Cardoso K., Serrano J., Casanova-Marti A., Blay MT., Pinent M., Ardevol A., Terra X., 2018. Article effects of an intermittent grape-seed proanthocyanidin (GSPE) treatment on a cafeteria diet obesogenic challenge in Rats. Nutrients, 10, 315. 31. Wang EH., Yu ZL., Bu YJ., Xu PW., Xi JY., Liang HY., 2019. Grape seed proanthocyanidin extract alleviates high-fat diet induced testicular toxicity in rats. RSC Adv, 9, 11842. 32. Mansouri E., Khorsandi L., Moaiedi MZ., 2015. Grape Seed Proanthocyanidin Extract Improved some of Biochemical Parameters and Antioxidant Disturbances of Red Blood Cells in Diabetic Rats. IJPR, 14, 329-334. 33. Sano A., Uchida R., Saito M., Shioya N., Komori Y., Tho Y., Hashizume N., 2007. Beneficial effects of grape seed extract on malondialdehyde-modified LDL. J Nutr Sci Vitaminol, 53, 174-182. 34. Ahmadvand H., Bagheris., Tamjidi-Poor A., Cheraghi M., Azadpour M., Ezatpour B., Moghadam S., Shahsavari G., Jalalvand M., 2016. Biochemical effects of oleuropein in gentamycin n-induced nephrotoxicity in rats. ARYA Atheroscler, 12, 87-93. 35. Blade C., Arola L., Salvado MJ., 2010. Hypolipidemic effects of proanthocyanidins and their underlying biochemical and molecular mechanisms. Mol Nutr Food Res, 54, 37-59. 36. Chamorro S., Viveros A., Centeno C., Romero C., Arija I., Brenes A., 2013. Effects of dietary grape seed extract on growth performance, amino acid digestibility and plasma lipids and mineral content in broiler chicks. Animal, 7, 555-561.

Broyler de Gentamisinin Olumsuz Etkilerine Karşı Üzüm Çekirdeği (Vitis vinifera L.) Ekstresinin Yem Katkısı Olarak Kullanımı

Yıl 2020, Cilt: 15 Sayı: 3, 271 - 278, 31.12.2020
https://doi.org/10.17094/ataunivbd.793341

Öz

Bu araştırmada, broilere gentamisin uygulasından dolayı performans parametreleri, organ ağırlıkları ve kan parametreleri üzerine oluşan olumsuz etkilerini gidermede üzüm çekirdeği (V. vinifera L) ektsresinin etkinliğini belirlemek amaçlanmıştır. Toplam 240 adet Ross 308 dişi civciv, her bir kafeste 10’ar adet olacak şekilde 6 tekerrürlü 4 gruba ayrıldı. Uygulama grupları; C grubu (kontrol grubu) sadece basal diyet ile beslendi; V grubu üzüm çekirdeği eksratı katkılı diyetle beslendi (100 mg/kg); G grubu basal diyetle beslendi ve gentamisin uygulandı (50 mg/kg vücut ağırlığı kas içi); V+G grubu ise üzüm çekirdeği eksratı katkılı diyetle beslendi ve gentamisin uygulandı. İstatistiksel olarak anlamlı olan en yüksek ağırlık artışı ve yem tüketimi 3, 4, 5 ve 6. Haftalar da C grubunda, en düşük ise G grubunda görüldü (P<0.05). En yüksek karkas ağırlığı C grubunda oldu. Aspartat aminotransferaz ve alanin aminotransferaz seviyelerinin en yüksek olduğu grup G grubuydu. En düşük kırmızı kan hücre değeri C grubunda oldu (P<0.05). Karaciğer ağırlığı G grubunda önemli ölçüde arttı. Bu araştırmada, gentamisin kullanımının etlik piliç sağlığına zarar verdiği ancak üzüm çekirdeği ekstratı eklenen yem ile bu etkilerin kısmen azaltılabileceği görüldü. Bu nedenle üzüm çekirdeği ekstratının yem katkı maddesi olarak kullanılabileceği kanaatine varıldı

Proje Numarası

Project Number: 2012/063

Kaynakça

  • 1. Islam NU., Khan MZ., Saleemi MK., Khan A., Bhatti SA., Yousaf M., Hassan ZU., 2011. Clinicopathological studies on gentamycin toxicity in White Leghorn commercial layers. Pak Vet J, 31, 305-308. 2. Akbarian A., Michiels J., Degroote J., Majdeddin M., Golian A., De Smet S., 2016. Association between heat stress and oxidative stress in poultry; mitochondrial dysfunction and dietary interventions with phytochemicals. J Anim Sci Biotechnol, 7, 37. 3. Lobo V., Patil A., Phatak A., Handra N., 2010. Free radicals, antioxidants and functional foods: impact on human health. Pharmacogn Rev, 4, 118-126. 4. Stambuk P., Tomaskovic D., Tomaz I., Maslov L., Stupic D., Karoglan Kontic J., 2016. Application of pectinases for recovery of grape seeds phenolics. 3 Biotech, 6, 224. 5. Cosansu S., Juneja VK., 2018. Growth of Clostridium perfringens in sous vide cooked ground beef with added grape seed extract. Meat Sci, 143, 252-256. 6. Özcan MM., Al Juhaimi F., Gülcü M., Uslu N., Geçgel Ü., 2017. Determination of bioactive compounds and mineral contents of seedless parts and seeds of grapes. S Afr J Enol Vitic, 38, 212-220. 7. Zhang S., Li L., Cui Y., Luo L., Zhou P., Sun B., 2017. Preparative high‐speed counter-current chromatography separation of grape seed proanthocyanidins according to degree of polymerization. Food Chem, 219, 399-407. 8. Kemperman RA., Gross G., Mondot S., Possemiers S., Marzorati M., Wiele TV., Vaughan EE., 2013. Impact of polyphenols from black tea and red wine/grape juice on a gut model microbiome. Food Res Int, 53, 659-669. 9. Charradi K., Mahmoudi M., Bedhiafi T., Jebari K., El May MV., Limam F., Aouani E., 2018. Safety evaluation, anti-oxidative and anti-inflammatory effects of subchronically dietary supplemented high dosing grape seed powder (GSP) to healthy rat. Biomed&Pharmacother, 107, 534-546. 10. Apaydin Yildirim B., Tunc MA., Yildirim S., Yildirim F., Kordali S., Ertekin A., Aktas Senocak E., 2017. The effect of Vitis vinifera L.cv. Merlot seed extract on gentamycin-induced nephrotoxicity in broilers. Europ Poult Sci, 81, 1-12. 11. Jing S., Zhang X., Yan LJ., 2015. Antioxidant activity, antitumor effect, and antiaging property of proanthocyanidins extracted from Kunlun Chrysanthemum flowers. Oxid Med Cell Longev, 2015, 1-10. 12. Kordalı Ş., Çakır A., Akcın TA., Mete E., Akcın A., Aydın T., Kılıç H., 2009. Antifungal and herbicidal properties of essential oils and n-hexane extracts of Achillea gypsicola Hub-Mor. and Achillea biebersteinii Afan. (Asteraceae). Ind Crops Prod, 29, 562-570. 13. SPSS (2004). SPSS for Windows Release 13.0. SPSS Inc. 14. Arafat N., Awadin WF., El-Shafei RA., Farag VME., Saleh RM., 2018. Protective Role of Moringa oleifera Leaves extract against gentamycin -induced nephro- and hepato-toxicity in chickens. AJVS, 58, 173-185. 15. Arivuchelvan A., Murugesan S., Mekala P., 2012. Antioxidant properties of Ocimum sanctum in broilers treated with high doses of gentamycin. IJDAD, 1, 143-146. 16. Khan I., Khan MZ., Saleemı MK., Javed I., Khan A., 2008. Pathological and Biochemical Effects of Intramuscular Gentamycin Administration in Chickens. Turk J Vet Anim Sci, 32, 345-351. 17. Javed U., Saleemi MK., Khan MZ., Khan A., Javed I., 2008. Pathological Effects of Gentamycin in Growing Broilers. Proceedings, The 15th Congress of FAVA. FAVA -OIE Joint Symposium on Emerging Diseases. 27-30 October 2008. Bangkok, Thailand, 17-18. 18. Javed U., Khan MZ., Saleemi MK., Khan A., Javed I., Rafique S., 2013. Toxico pathological effects of parenteral administration of gentamycin in growing Broilers. Int J Agric & Biol Eng, 15, 529-534. 19. Wang ML., Suo X., Gu JH., Zhang WW., Fang Q, Wang X., 2008. Influence of grape seed proanthocyanidin extract in Broiler chickens: Effect on chicken coccidiosis and antioxidant status. Poult Sci, 87, 2273-2280. 20. Abu Hafsa SH., Ibrahim SA., 2018. Effect of dietary polyphenol- rich grape seed on growth performance, antioxidant capacity and ileal microflora in broiler chicks. J Anim Physiol Anim Nutr, 102, 268-275. 21. Ismail AM., Sedki AA., Abdallah AG., 2003. Influence of black seed, garlic and onion supplementation on reproductive performance in rabbits. Egypt J Agric Res, 81, 1193-1207. 22. Kermauner A., Laurencic A., 2008. Supplementation of rabbit diet with chestnut wood extract: Effect on in vitro gas production from two sources of protein. Proceedings of the 9thWorld Rabbit Congress, 10–13 Jun, Verona, Italy, 689-693. 23. Taranu I., Marin DE., Palade M., Pistol GC., Chedea VS., Gras MA., Rotar C., 2019. Assessment of the efficacy of a grape seed waste in counteracting the changes induced by aflatoxin B1 contaminated diet on performance, plasma, liver and intestinal tissues of pigs after weaning. Toxicon, 162, 24-31. 24. Brenes A., Viveros A., Goni I., Centeno C., Sa yago-Ayerdy SG., Arija I., Saura-Calixto F., 2007. Effect of grape pomace concentrate and vitamin E on digestibility of polyphenols and antioxidant activity in chickens. Poult Sci, 87, 307-316. 25. Hajati H., Hassanabadi A., Golian A., Nassiri-Moghaddam H., Nassiri MR., Safari R., 2015. The effect of grape seed extract and vitamin C feed supplements carcass characteristics, gut morphology and ileal microflora in broiler chickens exposed to chronic heat stress. IJAS, 5, 155-165. 26. Erişir Z., Şimşek ÜG., Özçelik M., Baykalır Y., İflazoğlu Mutlu S., Çiftçi M., 2018. Effects of dietary grape seed on performance and some metabolic assessments in Japanese quail with different plumage colors exposed to heat stress. R Bras Zootec, 47, 1-10. 27. Center SA., 2007. Interpretation of liver enzymes. Vet Clin N Am-Small, 37, 297-333. 28. Saleemi MK., Khan MZ., Khan A., Javed I., 2009. Pathological effects of gentamycin administered intramuscularly to day old broiler chicks. Exp Toksicol Pathol, 61, 425-432. 29. Rajput SA., Sun L., Zhang N., Khalil MM., Gao X., Ling Z., Zhu L., Khan FA., Zhang J., Qi D., 2017. Ameliorative effects of grape seed proanthocyanidin extract on growth performance, immune function, antioxidant capacity, biochemical constituents, liver histopathology and aflatoxin residues in Broilers Exposed to Aflatoxin B1. Toxins, 9, 371-391. 30. Gines I., Gil-Cardoso K., Serrano J., Casanova-Marti A., Blay MT., Pinent M., Ardevol A., Terra X., 2018. Article effects of an intermittent grape-seed proanthocyanidin (GSPE) treatment on a cafeteria diet obesogenic challenge in Rats. Nutrients, 10, 315. 31. Wang EH., Yu ZL., Bu YJ., Xu PW., Xi JY., Liang HY., 2019. Grape seed proanthocyanidin extract alleviates high-fat diet induced testicular toxicity in rats. RSC Adv, 9, 11842. 32. Mansouri E., Khorsandi L., Moaiedi MZ., 2015. Grape Seed Proanthocyanidin Extract Improved some of Biochemical Parameters and Antioxidant Disturbances of Red Blood Cells in Diabetic Rats. IJPR, 14, 329-334. 33. Sano A., Uchida R., Saito M., Shioya N., Komori Y., Tho Y., Hashizume N., 2007. Beneficial effects of grape seed extract on malondialdehyde-modified LDL. J Nutr Sci Vitaminol, 53, 174-182. 34. Ahmadvand H., Bagheris., Tamjidi-Poor A., Cheraghi M., Azadpour M., Ezatpour B., Moghadam S., Shahsavari G., Jalalvand M., 2016. Biochemical effects of oleuropein in gentamycin n-induced nephrotoxicity in rats. ARYA Atheroscler, 12, 87-93. 35. Blade C., Arola L., Salvado MJ., 2010. Hypolipidemic effects of proanthocyanidins and their underlying biochemical and molecular mechanisms. Mol Nutr Food Res, 54, 37-59. 36. Chamorro S., Viveros A., Centeno C., Romero C., Arija I., Brenes A., 2013. Effects of dietary grape seed extract on growth performance, amino acid digestibility and plasma lipids and mineral content in broiler chicks. Animal, 7, 555-561.
Toplam 1 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Sağlık Kurumları Yönetimi
Bölüm Araştırma Makaleleri
Yazarlar

Muhammet Ali Tunç

Proje Numarası Project Number: 2012/063
Yayımlanma Tarihi 31 Aralık 2020
Yayımlandığı Sayı Yıl 2020 Cilt: 15 Sayı: 3

Kaynak Göster

APA Tunç, M. A. (2020). The Use of Grape Seed (Vitis vinifera L.) Extract as Feed Additive Against Negative Effects of Gentamycin in Broiler. Atatürk Üniversitesi Veteriner Bilimleri Dergisi, 15(3), 271-278. https://doi.org/10.17094/ataunivbd.793341
AMA Tunç MA. The Use of Grape Seed (Vitis vinifera L.) Extract as Feed Additive Against Negative Effects of Gentamycin in Broiler. Atatürk Üniversitesi Veteriner Bilimleri Dergisi. Aralık 2020;15(3):271-278. doi:10.17094/ataunivbd.793341
Chicago Tunç, Muhammet Ali. “The Use of Grape Seed (Vitis Vinifera L.) Extract As Feed Additive Against Negative Effects of Gentamycin in Broiler”. Atatürk Üniversitesi Veteriner Bilimleri Dergisi 15, sy. 3 (Aralık 2020): 271-78. https://doi.org/10.17094/ataunivbd.793341.
EndNote Tunç MA (01 Aralık 2020) The Use of Grape Seed (Vitis vinifera L.) Extract as Feed Additive Against Negative Effects of Gentamycin in Broiler. Atatürk Üniversitesi Veteriner Bilimleri Dergisi 15 3 271–278.
IEEE M. A. Tunç, “The Use of Grape Seed (Vitis vinifera L.) Extract as Feed Additive Against Negative Effects of Gentamycin in Broiler”, Atatürk Üniversitesi Veteriner Bilimleri Dergisi, c. 15, sy. 3, ss. 271–278, 2020, doi: 10.17094/ataunivbd.793341.
ISNAD Tunç, Muhammet Ali. “The Use of Grape Seed (Vitis Vinifera L.) Extract As Feed Additive Against Negative Effects of Gentamycin in Broiler”. Atatürk Üniversitesi Veteriner Bilimleri Dergisi 15/3 (Aralık 2020), 271-278. https://doi.org/10.17094/ataunivbd.793341.
JAMA Tunç MA. The Use of Grape Seed (Vitis vinifera L.) Extract as Feed Additive Against Negative Effects of Gentamycin in Broiler. Atatürk Üniversitesi Veteriner Bilimleri Dergisi. 2020;15:271–278.
MLA Tunç, Muhammet Ali. “The Use of Grape Seed (Vitis Vinifera L.) Extract As Feed Additive Against Negative Effects of Gentamycin in Broiler”. Atatürk Üniversitesi Veteriner Bilimleri Dergisi, c. 15, sy. 3, 2020, ss. 271-8, doi:10.17094/ataunivbd.793341.
Vancouver Tunç MA. The Use of Grape Seed (Vitis vinifera L.) Extract as Feed Additive Against Negative Effects of Gentamycin in Broiler. Atatürk Üniversitesi Veteriner Bilimleri Dergisi. 2020;15(3):271-8.