Sıçanlarda Nikel Genotoksikasyonuna Karşı Bromelainin Etkisi

Yıl 2022, Cilt: 17 Sayı: 1, 26 - 30, 29.04.2022

Füsun Temamoğulları Pinar Aksu Kılıçle Şükrü Gürler Zozan Garip

Öz

Bu çalışmanın amacı, nikel sülfat genotoksikasyonuna bromelainin karşı koruyucu rolünü araştırmaktır. Çalışmada, ortalama 200 ± 20 gr ağırlığında yirmi dört sağlıklı yetişkin erkek Sprague dawley sıçan kullanıldı. Rastgele olacak şekilde dört grup (n = 6) oluşturuldu. Sıçanlar bir kontrol ve üç deney grubu: Grup 1 (Kontrol), Grup 2 (Nikel sülfat 20 mg/kg, ip), Grup 3 (Bromelain 20 mg/kg, oral gavaj), Grup 4 (Nikel sülfat, 20 mg/kg, ip + bromelain, 20 mg/kg, oral gavaj). On günlük deney periyodundan sonra hayvanlar on birinci gün ötenazi edildi. 11. günde ötenazi edilen hayvanlardan femur kemikleri alındı ve mikronükleus protokolü uygulandı. Protokolün sonunda MNPCE (mikronükleuslu polikromatik eritrositler) ve PCE (polikromatik eritrositler) sayıldı. Sayılan değerlerin istatistiksel sonuçları belirlendi. Tüm gruplar sadece MNCPE parametresine göre karşılaştırıldı. MNCPE seviyesi, bromelainli grupta, sadece nikel sülfatlı grubuna kıyasla istatistiksel olarak anlamlıydı (P < ,05). Elde edilen veriler, bromelainin 20 mg/kg dozunda uygulanması, 20 mg/kg dozunda uygulanan nikelsülfatin oluşturduğu genotoksik etkilerini potansiyel olarak önleyici olmadığını göstermiştir.

Anahtar Kelimeler

Bromelain, Genotoksisite, Nikel sülfat, Rat

Destekleyen Kurum

HARRAN ÜNİVERSİTESİ - BİLİMSEL ARAŞTIRMA PROJELERİ BİRİMİ

Proje Numarası

18029

Effect of Bromelain Against Nickel Genotoxication in Rats

Öz

In this study, we aimed to investigate the protective role of bromelain against nickel sulfate genotoxication. Twenty-four healthy adult male Sprague Dawley rats with an average weight of 200 ± 20 g were used in the study. These were divided randomly into four groups (n = 6), including one control and three experimental groups: Group 1 (control), Group 2 (nickel sulfate 20 mg/kg, intraperitoneal [IP]), Group 3 (Bromelain 20 mg/kg, oral gavage ), Group 4 (nickel sulfate 20 mg/kg IP + bromelain 20 mg/kg oral gavage ). After the ten-day experimental period, the animals were euthanized on the 11th day. Femoral bones were taken from animals dissected on day 11, and micronucleus protocol was applied. At the end of the protocol, micronucleated polychromatic erythrocytes (MNPCE) and polychromatic erythrocytes (PCE) were counted. The statistical results of the counted values were determined. The groups were compared only according to MNCPE, the level of which was statistically significant in the group with bromelain than in the group with nickel sulfate alone (P < .05). The data obtained suggested that bromelain at the administered dose (20 mg/kg) is not potentially preventive to the genotoxic effects of nickel sulfate at the administered dose (20 mg/kg).

Anahtar Kelimeler

Bromelain, Genotoxicity, Nickel sulphate, Rat

Proje Numarası

18029

Kaynakça

• 1. Cempel M, Nikel G. Nickel: A review of its sources and environmental toxicology. Pol J Environ Stud. 2006;15(3):375-382.
• 2. Adjroud O. The toxic effects of nickel chloride on liver, erythropoiesis, and development in Wistar albino preimplanted rats can be reversed with selenium pretreatment. Environ Toxicol. 2013;28(5):290-298. [Crossref]
• 3. Owumi SE, Olayiwola YO, Alao GE, Gbadegesin MA, Odunola OA. Cadmium and nickel co-exposure exacerbates genotoxicity and not oxido- inflammatory stress in liver and kidney of rats: Protective role of omega-3 fatty acid. Environ Toxicol. 2020;35(2):231-241. [Crossref]
• 4. Cameron KS, Buchner V, Tchounwou PB. Exploring the molecular mechanisms of nickel-induced genotoxicity and carcinogenicity: a literature review. Rev Environ Health. 2011;26(2):81-92. [Crossref]
• 5. Kaya S. Veteriner Toksikoloji. 3th ed., 50-528, Medisan Yayınevi, Ankara, 2014.
• 6. Das KK, Das SN, Dhundasi SA. Nickel, its adverse health effects & oxidative stress. Indian J Med Res. 2008;128(4):412-425.
• 7. Bhardwaj H, Singh C, Nayyar S. Assessment of adverse effects of lead, nickel and cadmium on biochemical parameters, antioxidants status and metallothionein expression in buffaloes slaughtered at local abattoir. Indian J Anim Res. 2021;1-8. DOI:10.18805/IJAR.B-4242. [Crossref]
• 8. Badr AN, Abdel-Razek AG, Youssef MM, Shehata MG, Hassanein MM, Amra HA. Natural antioxidants: preservation roles and mycotoxicological safety of food. Egypt J Chem. 2021;64:4-5.
• 9. Rathnavelu V, Alitheen NB, Sohila S, Kanagesan S, Ramesh R. Potential role of bromelain in clinical and therapeutic applications. Biomed Rep. 2016;5(3):283-288. [Crossref]
• 10. Şehirli AÖ, Sayiner S, Savtekin G, Velioğlu-Öğünç A. Protective effect of bromelain on corrosive burn in rats. Burns. 2021;47(6):1352-1358. [Crossref]
• 11. Huang CW, Lin IJ, Liu YM, Mau JL. Composition, enzyme and antioxidant activities of pineapple. Int J Food Prop. 2021;24(1):1244-1251. [Crossref]
• 12. Kaya S. Veteriner Farmakoloji. 5 th ed., 291, Medisan Yayınevi, Ankara, 2013.
• 13. Taşkın A, Tarakçıoğlu M, Ulusal H, Örkmez M, Taysı S. Idarubicin-bromelain combination sensitizes cancer cells to conventional chemotherapy. Iran J Basic Med Sci. 2019;22(10):1172.
• 14. Kurdi LAF. The ability of fresh pineapple juice in reducing the cytogenetic effect of ifosfamide on the bone marrow cells of male albino mice. Adv Environ Biol. 2016;10:120-130.
• 15. Izquierdo-Vega JA, Morales-González JA, SánchezGutiérrez M, et al. Evidence of some natural products with antigenotoxic effects. Part 1: fruits and polysaccharides. Nutrients. 2017;9(2):102. [Crossref]
• 16. Platt KL, Edenharder R, Aderhold S, Muckel E, Glatt H. Fruits and vegetables protect against the genotoxicity of heterocyclic aromatic amines activated by human xenobiotic metabolizing enzymes expressed in immortal mammalian cells. Mutat Res Genet Toxicol Environ Mutagen. 2010;703(2):90-98. [Crossref]
• 17. Stopper H, Schinzel R, Sebekova K, Heidland A. Genotoxicity of advanced glycation end products in mammalian cells. Cancer Lett. 2003;190(2):151-156. [Crossref]
• 18. Pari L, Amudha K. Hepatoprotective role of naringin on nickel-induced toxicity in male Wistar rats. Eur J Pharmacol. 2011;650(1):364- 370. [Crossref]
• 19. Al-Otaibi WR, Virk P, Elobeid M. Ameliorative potential of stem bromelain on lead-induced toxicity in Wistar rats. Acta Biol Hung. 2015;66(2):149-160. [Crossref]
• 20. Eckert K, Grabowska E, Stange R, Schneider U, Eschmann K, Maurer HR. Effects of oral bromelain administration on the impaired immunocytotoxicity of mononuclear cells from mammary tumor patients. Oncol Rep. 1999;6(6):1191-1200. [Crossref]
• 21. Schmid W. The micronucleus test. Mutat Res. 1975;31:9-15. [Crossref]
• 22. Botta A, Lehucher Michel MP, Di Giorgio C. Genotoxic effects of nickel sulfate in cultured human lymphocytes. Med Sci Res. 1994;22:709-710.
• 23. Rizvi A, Parveen S, Khan S, Naseem I. Nickel toxicology with reference to male molecular reproductive physiology. Reprod Biol. 2020;20(1):3-8. [Crossref]
• 24. Salimi A, Jamali Z, Atashbar S, Khezri S, Ghorbanpour AM, Etefaghi N. Pathogenic mechanisms and therapeutic implication in nickel- induced cell damage. Endocr Metab Immune Disord Drug Targets. 2020;20(7):968-984. [Crossref]
• 25. Seoane AI, Dulout FN. Genotoxic ability of cadmium, chromium and nickel salts studied by kinetochore staining in the cytokinesis- blocked micronucleus assay. Mutat Res Genet Toxicol Environ Mutagen. 2001;490(2):99-106. [Crossref]
• 26. M’Bemba-Meka P, Lemieux N, Chakrabarti SK. Nickel compound-induced DNA single-strand breaks in chromosomal and nuclear chromatin in human blood lymphocytes in vitro: role of oxidative stress and intracellular calcium. Mutat Res Genet Toxicol Environ Mutagen. 2005;586(2):124-137. [Crossref]
• 27. Oller AR, Erexson G. Lack of micronuclei formation in bone marrow of rats after repeated oral exposure to nickel sulfate hexahydrate. Mutat Res Genet Toxicol Environ Mutagen. 2007;626(1-2):102-110. [Crossref]
• 28. Şekeroğlu V, Şekeroğlu ZA. Genetik toksisite testleri. TÜBAV Bilim Derg. 2011;4:221-229.
• 29. Polat F, Bingöl G, Turaclar N. An investigation of micronucleus induction by butylated hydroxytoluene in wistar rat bone marrow cells. Kafkas Univ Vet Fak Derg. 2014;20(4):527-531.
• 30. Kurdi LAF. Effectiveness of preventive of fresh pineapple juice against the genotoxicity effects of ifosfamide on albino mice. Adv Environ Biol. 2016;10:276-293.
• 31. Özparlak H, Arslan A, Güler GÖ. Organik insektisit Fipronil’in genotoksik etkilerinin civciv mikronukleus test sisteminde belirlenmesi. Selçuk Univ Fen Fak Fen Derg. 2011;2(37):1-8.
• 32. Ulu H, Kılıçle PA. Fare kemik iliği hücrelerinde siklofosfamid tarafından indüklenen genotoksisiteye karşı tarhun (Artemisia dracunculus L.) yaprak ekstraktının olası koruyucu etkisinin mikronükleus testi ile belirlenmesi. Caucasian Med J. 2020;7(2):92-108. [Crossref]
• 33. Ferguson LR, Philpott M. Nutrition and mutagenesis. Annu Rev Nutr. 2008;28:313-329. [Crossref]
• 34. Karabulut H, Gülay MŞ. Antioksidanlar. MAEU Vet Fak Derg. 2016;1(1):65-76. [Crossref]
• 35. Lee JH, Lee JT, Park HR, Kim JB. The potential use of bromelain as a natural oral medicine having anticarcinogenic activities. Food Sci Nutr. 2019;7(5):1656-1667. [Crossref]
• 36. Castro e Braga C, Teixeira LSD, Nascimento WM, et al. Use of bromelain in the quality of frozen/thawed goat Sêmen. Cienc Anim. 2020;30:261-265.
• 37. Amini A, Masoumi-Moghaddam S, Morris DL. Bromelain. In “Utility of Bromelain and N-Acetylcysteine in Treatment of Peritoneal Dissemination of Gastrointestinal Mucin-Producing Malignancies”, Springer, Cham, 2016: 63-80. [Crossref]
• 38. Stopper H, Schinzel R, Sebekova K, Heidland A. Genotoxicity of advanced glycation and products in mammalian cells. Cancer Lett. 2003;190(2):151-156. [Crossref]
• 39. Sen CC, Temamogullari F, Yumusak N, Kirmit A. Bromelain prevented nickel-induced testicular toxicity via suppression of sperm DNA fragmentation and sperm quality alterations in rats. Med Weter. 2019;75(5):558-563. [Crossref]
• 40. Ikken Y, Morales P, Martínez A, Marín ML, Haza AI, Cambero MI. Antimutagenic effect of fruit and vegetable ethanolic extracts against N-nitrosamines evaluated by the Ames test. J Agric Food Chem. 1999;47(8):3257-3264. [Crossref]
• 41. Báez R, Lopes MT, Salas CE, Hernandez M. In vivo antitumoral activity of stem pineapple (Ananas comosus) bromelain. Planta Med. 2007;73(13):1377-1383. [Crossref]
• 42. Sah BNP, Vasiljevic T, McKechnie S, Donkor ON. Effect of refrigerated storage on probiotic viability and the production and stability of antimutagenic and antioxidant peptides in yogurt supplemented with pineapple peel. Int J Dairy Sci. 2015;98(9):5905-5916. [Crossref]
• 43. Garip Z, Temamogullari F. Silkworm in pharmacology and toxicology. Int IJVAR. 2021;4(1):34-38.
• 44. Prisby RD. Mechanical, hormonal and metabolic influences on blood vessels, blood flow and bone. J Endocrinol. 2017;235(3):R77-R100. [Crossref]
• 45. Athar M, Mısra M, Srivastava RC. Evaluation of chelating drugs on the toxicity, excretion, and distribution of nickel in poisoned rats. Toxicol Sci. 1987;9(1):26-33. [Crossref]
• 46. Doreswamy K, Shrilatha B, Rajeshkumar T. Nickel-induced oxidative stress in testis of mice: evidence of DNA damage and genotoxic effects. J Androl. 2004;25:996-1003. [Crossref]
• 47. Singh M, Verma Y, Rana SVS. Attributes of oxidative stress in the reproductive toxicity of nickel oxide nanoparticles in male rats. Environ Sci Pollut Res. 2022;29(4):5703-5717. [Crossref]
• 48. Karakaya E. Testis Torsiyonunun Detorsiyonu Sonrasında Oluşan Iskemi/ Reperfüzyon Hasarının Rosuvastatin Ile Önlenebilirliği. Dokuz Eylül Üniversitesi, Sağlık Bilimleri Enstitüsü, Türkiye, 2006.