Sığırların Verim Özellikleri Üzerine Etkili Önemli Moleküler Markörler

Öz

Moleküler genetik teknikler bazı genlerde genetik varyasyonun tanımlanmasını ve QTL’deki varyasyonla verim özellikleri arasındaki ilişkileri incelemeye imkanlar sağlamaktadır. Amaç hayvanın genetik değerini daha yüksek bir doğrulukla tahmin etmek ve bunun sonucu olarak seleksiyonla elde edilen genetik kazancı artırmaktır. Son yıllarda sığırların ıslahında MAS kullanımıyla ilgili moleküler genetik tabanlı çalışmaların sayısı günden güne artmaktadır. Hayvancılık alanında yapılan bir çok moleküler genetik çalışmalarında CSN2, CSN3, CSN1S1, LGB, DGAT1, BH, IGF, GHRH-R, LEP, Pit-1, PRL, Calpain ve Calpastatin gibi genler moleküler markör olarak incelenmektedir. Bu derlemede amaç sığırların ıslahına yönelik verim özellikleriyle ilişkisi tespit edilmiş önemli moleküler DNA markörlerini incelemektir.

Anahtar Kelimeler

• Moleküler DNA markörleri, MAS, Verim özellikleri, Genetik çeşitlilik

Kaynakça

1. Aschaffenburg, and R. Dewry, J. 1957. lmproved method for the preparation of crystalline b-lactoglobulin and a-lactalbumin from cow's milk. Biochemistry 65: 273-277.
2. Ashwell, M. S., Rexroad Jr., C.E., Miller, R.H., Van Raden, P. M. and Da, Y. 1997. Detection of loci affecting milk production and health traits in an elite US Holstein population using microsatellite markers. Animal Genetics 28:216-222.
3. Bass, J.J., Oldham, J.M., Hodgkinson, S.C., Fowke, P.J., Sauerwein, H., Molan, P., Breier, B.H., Gluckman, P.D. 1991. Influence of nutrition and bovine growth hormone (GH) on hepatic GH binding, insulin-like growth factor-I and growth of lambs. J. Endocrinol, 128(2):181-186.
4. Bauman, D.E., Eppard, P.J., DeGeeter, M.J. and Lanza, G. M. 1985. Response of high producing dairy cows to long term treatment with pituitary- and recombinant-growth hormone. J. Dairy Sci. 68:1352-1362.
5. Bauman, D.E. 1992. Bovine somatotropin: review of an emerging animal technology. J. Dairy Sci. 75 (12):3432-3451.
6. Beckmann, J.S. and Soller, M. 1983. Restriction fragment length polymorphisms in genetic improvement: methodologies, mapping and costs. Theoretical and Applied Genetics, 67 : 35-43.
7. Bishop, M.D., Hawkins, G.A., and Keener, C.L., 1995. Use of DNA markers in animal selection. Theriogenology, 43:6170.
8. Brenneman, R.A., Davis, S.K., sanders, J.O., Burns, B.M., Wheeler, T.C., Turner, J.W. and Taylor, J.F., 1996. The polled locus maps to BTA1 in a Bos indicusx Bos Taurus cross. J. Hered. 87:156-161.

9. Brym, P., Kaminski, S. and Wojcik, E. 2005. Nucleotide sequence polymorphism within exon 4 of the bovine prolactin gene and its associations with milk performance traits. J. Appl.Genet. 45(2): 179–185.
10. Cases, S., Smith, S.J., Zheng, Y.W. and Myers, H.M. 1998. Identification of a gene encoding an acyl CoA:diacylglycerol acyltransferase, a key enzyme in triacylglycerol synthesis. Proc. Natl. Acad. Sci. USA 95: 13018-13023.
11. Chen, H.Y., Zhang, Q., Yin, C.C., Wang, C.K., Gong, W.J. and Mei, G. 2006. Detection of quantitative trait loci affecting milk production traits on bovine chromosome 6 in a Chinese Holstein population by the daughter design. J. Dairy Sci. 89(2):782-790.
12. Chrenek, P., Kmet, J., Sakowski, T., Vasicek, D., Huba, J. and Chrenek, J., 1998. Relationships of growth hormone genotypes with meat production traits of Slovak Pied bulls. Czech J. Anim. Sci. 43(12):541-544.
13. Citek, J., Rehout, V. and Neubauerova, V., 2001. Allele frequency at PRL (prolactin) and LGB (lactoglobulin beta) genes in Red cattle breeds from Central Europe and in other breeds. Czech J. Anim. Sci. 46(10):433-438.
14. Cosier, V. 2006. RFLP/HinfI polymorphism between exon 5 and exon 6 of the Pit-1 gene in Romanian Simmental cattle. Buletinul Universitatii de Stiinte Agricole si Medicina Veterinara Cluj-Napoca, Seria Zootehnie si Biotehnologii 62:219-222 (Abst.).
15. Curi, R.A., Palmieri, D.A., Suguisawa, L., de Oliviera,H.N., Silveira, A.C. and Lopes, C.R., 2006. Growth and carcass traits associated with GH1/AluI and POU1F1/HinfI gene polymorphisms in Zebu and crossbred beef cattle. Genetics and Molecular Biology, 29(1):56-61.
16. Di Stasio, L., Brugiapaglia, A., Destefanis, G., Albera, A. And Sartore, S., 2003. GH1 as candidate gene for variability of meat production traits in Piemontese cattle. J. Anim. Breed.Genet. 120 (5): 358-361.
17. Drogemuller, C., Hamann, H. and Distl, O. 2001. Candidate gene markers for litter size in different German pig lines. J. Anim. Sci. 79: 2565-2570.
18. Dybus A, 2002b. Associations of growth hormone GH and prolactin PRL genes polymorphism with milk production traits in Polish Black and White cattle. Anim. Sci. Pap. Rep. 20: 203–212.
19. Dybus, A., 2002a. Associations between Leu/Val polymorphism of growth hormone gene and milk production traits in Blackand-White cattle. Arch. Tierz. Dummerstorf 45 (5): 421-428.
20. Dybus, A., Grzesiak, W., Kamieniecki, H., Szatkowska, I., Sobek, Z., Blaszczyk, P., Czerniawska-Piatkowska, E., Zych, S. and Muszynska, M., 2005. Association of genetic variants of bovine prolactin with milk production traits of Black-andWhite and Jersey cattle. Archives of Anim. Breed. 48 (2): 149-156 (Abst).
21. Dybus, A., Kmiec, M., Sobek, Z. and Wisniewski, B., 2003b. Associations between polymorphism of the growth hormone gene and production traits of Limousine cattle. Medycyna Weterynaryjna, 59 (2): 133-136 (Abst).
22. Dybus, A., Kmiec, M., Sobek, Z., Pietrzyk, W. and Wisniewski, B. 2003a. Associations between polymorphisms of growth hormone releasing hormone (GHRH) and pituitary transcription factor (PIT1) genes and production traits of Limousine cattle. Arch. Tierz. Dummerstorf 46(6): 527-534.
23. Edwards, M.D. and Page, N.J., 1994. Evaluation of markerassisted selection through computer simulation. Theor. Appl. Genet. 88:376-382.
24. Fries, R., Eggen A. and Womack J.E. 1993. The bovine genome map. Mammal. Genome. 4: 405-428.
25. Ge, W., Davis, M.E., Hines, H.C., Irvin K.M. and Simmen, C.M. 2003. Association of single nucleotide polymorphisms in the growth hormone and growth hormone receptor genes with serum insulin-like growth factor I concentration and growth traits in Angus cattle. J. Anim. Sci., 81:641–648.
26. Grisart, B., Coppieters, W., Farnir F. and Karim L., 2002. Positional candidate cloning of a QTL in dairy cattle: identification of a missense mutation in the bovine DGAT1 gene with major effect on milk yield and composition. Genome Res. 12: 222-231
27. Grisart, B., Farnir, F., Karim L., Cambisano, N, Kim, J., Kvasz, A., Mni, M., Simori, P., Frere, J., Coppieters, W. and Georges, M. 2004. Genetic and fuctional confirmation of the causality of the DGAT1 K232A quantitative trait nucleotide in affecting milk yield and composition. Proc. Natl. Acad. Sci. USA 101:2308-2403.
28. Grochowska, R., Lunden, A., Zwierzchowski, L., Snochowski, M. and Oprzadek, J. 2001. Association between gene polymorphism of growth hormone and carcass traits in dairy bulls. Anim. Sci. 72: 441-447.
29. Groza, I., Pamfil, D., Cenariu, M. and Chereji, R. 2005. Genetic markers in dairy cattle milk production. Buletinul Universitatii de Stiinte Agricole si Medicina Veterinara ClujNapoca, Seria Medicina Veterinara 62 : 422-426. (Abst.).
30. Hallerman, E.M., Theilmann, J.L., Beckmann, J.S., Soller M. and Womack, J.E. 1988. Mapping of bovine prolactin and rhodopsin genes in hybrid somatic cells. Anim. Genet. 19: 123–131.
31. Horseman, N.D., Zhao, W., Montecino-Rodriguez, E., Tanaka, M., Nakashima, K., Engle, S.J. 1997. Defective mammopoiesis, but normal hematopoiesis, in mice with a targeted disruption of the prolactin gene. EMBO J., 16: 6926–6935.
32. Hu, C.C. and Mao, F.C., 1995, Kappa-casein genotyping and its correlation with milk producing ability of Holstein bulls. Taiwan journal of Veterinary Medicine and Animal Husbundary, 65:3, 247-254, (Abst.).
33. Ihara, N., Takasuga, A., Mizoshita, K. and Takeda, H. 2004. A comprehensive genetic map of the cattle genome based on 3802 microsatellites, Genome Research 14: 1987-1998.
34. Kashi, Y., Hallerman, E. and Soller, M., 1990. Marker asisted selection of candidate bulls for progeny testing programs. Anim. Prod. 52:21-31.
35. Kaupe, B., Winter A., Fries, R. and Erhardt, G. 2004. DGAT1 polymorphism in Bos indicus and Bos taurus cattle breeds. J. Dairy Res. 71: 182-187.
36. Khatami, S.R., Lazebny, O.E., maksimenko, V.F. and Sulimova, G.E., 2005. Association of DNA polymorphisms of the growth hormone and prolactin genes with milk productivity in Yaroslavl and Black-and-White cattle. Russian J. of Genetics, 41 (2):167-173 Abst.
37. Klauzinska, M. 2002. Polymorphism of 5’-flanking regions of genes GH, GHRH, prolactin, and cattle miostatin. PhD thesis, Institute of Anim.Genet. Breed., Jastrzebiec, Poland.
38. Komisarek, J., Szyda, j., Michalak, A. And Dorynek, Z., 2005. Impact of leptin gene polymorphisms on breeding value for milk production traits in cattle. J. Anim. Feed Sci. 14 (3) 491-500.
39. Kulig, H., 2005a. Association between leptin combined genotypes and milk performance traits of Polish Black-and-White cows. Archives of Anim. Breed. 48 (6): 547-554 (Abst).
40. Kulig, H., 2005b. Associations between leptin gene polymorphism and some milk performance traits of cattle. J. Anim. Feed Sci. 14 (2) 235-243.
41. Lagziel, A., Lipkin, E. and Soller, M. 1996. Association between SSPC haplotypes at the bovine growth hormone gene and milk protein percentage. Genetics, 142: 946-951.
42. Lagziel, A., Lipkin, E., Ezra, E., Soller, M. and Weller, J.I. 1999. An MspI polymorphism at the bovine growth hormone (bGH) gene is linked to a locus affecting milk protein percentage. Animal Genetics, 30: 296-299.
43. Leifers, S.C., Veerkamp, R.F., Te Pas, M.F.W., Chilliard, Y. And Van der Lende, T. 2005. Genetics and Physiology of leptin in periparturient dairy cows. Domestic Animal Endocrinology 29: 227-238.
44. Li, J.T., Wang, A.H., Chen, P., Li, H.B., Zhang, C.S. and Du, L.X., 2006. Relationship between the polymorphisms of 5' regulation region of prolactin gene and milk traits in Chinese Holstein dairy cows. Asian-Australasian Journal of Animal Sciences 19 (4) : 459-462. (Abst.).
45. Lucy, M.C., Hauser, S.D., Eppard, P.J., Krivi, G.G., Clark, J.H., Bauman, D.E. and Collier, R.J., 1993. Variants of somatotropin in cattle- gene frequencies in major dairy breeds and associated milk-production. Domestic Animal Endocrinology, 10(4): 325-333. (Abst).
46. Macajova, M., Lamasova, D. and Zeman, M. 2004. Role of Leptin in Farm Animals: a Review. J. Vet. Med. A 51, 157–166.
47. Machnik, G and Lechniak, D., 2000. The impact of growth hormone (GH) on male reproduction. Medycyna Weterynaryjna, 56 (4): 218-221 (Abst).
48. Madeja, Z., Adamowicz, T., Chmurzynska, A., Jankowski, T., Melonek, J., Switonski, M. and Strabel, T., 2004. effect of leptin gene polymorphism on breeding value form ilk production traits. J. Dairy Sci. 87 (11):3925-3927.
49. Mattos, K.K., Del-Lama, S.N., Martinez, M.L. and Freitas, F. 2004. Association of bGH and Pit-1 gene variants with milk production traits in dairy Gyr bulls. Pesq. Agropec. Bras, 39 (2): 147-150.
50. Messina, M., Vrech, E., Pezzi, P. and Prandi, A. 1999. Genetic markers associated with the somatotropin axis and milk protein polymorphism. Scienza-e-Tecnica-Lattiero- Casearia 50 231-240.
51. Miceikiene, I., Peciulaitiene, N., Baltrenaite, I., Skinkyte, R. and Indriulyte, R. 2006. Association of cattle genetic markers with performance traits. Biologija, 1:24-29.
52. Mitra, A., Schlee, P., Balakrishnan C.R. and Pirchner F. 1995.Polymorphism at growth hormone and prolactin loci in Indian cattle and buffalo. J. Anim. Breed. Genet. 112: 71–74.
53. Moussavi, A.H., Ahouei, M., Nassiry, M.R. and Javadmanesh, A., 2006. Association of leptin polymorphism with production, reproduction and plasma glucose level in Iranian Holstein cows. Asian-Australasian J. of Anim. Sci. 19 (5):627-631 (Abst).
54. Özdemir, M. 2001. Çeşitli Sığır Irklarında Süt Protein Polimorfizmi ve Verim Özellikleri ile İlişkisi. Atatürk Üniversitesi, Fen Bilimleri Enstitüsü, Y.Lisans Tezi. Erzurum.
55. Pal, A., Chakravarty, A.K., Bhattacharya, T.K. and Sharma, A. 2005. Polymorphism of growth hormone gene and its association with expected milk production traits in dairy bulls. J. Appl. Anim. Res. 27(1): 29-33.
56. Peel, C. J., and Bauman, D. E. 1987. Somatotropin and lactation. J. Dairy Sci. 70:474-486.
57. Pomp, D., Zou, T., Clutter, A.C. and BarendseW. 1997. Rapid communication: Mapping of leptin to bovine chromosome 4 by linkage analysis of a PCR-based polymorphism. J. Anim. Sci. 75:1427.
58. Rincon, G., Armstrong, E. and Postiglioni, A., 2006. Analysis of population structure of Uruguayan Creole cattle as inferred from milk major gene polymorphisms. Genet. Mol. Biol. 29(3) 491-495.
59. Rocha, J. L., Baker, J. F., Womack, J. E., Sanders, J. O. and Taylor, J. F. 1992. Statistical associations between restriction fragment length polymorphisms and quantitative traits in beef cattle. J. Anim. Sci. 70: 3360–3370.
60. Ron, M., Feldmesser, E., Golik, M., Tager-Cohen, I., Kliger, D., Reiss, V., Domochovsky, R., Alus, O., Seroussi, E., Ezra, E., Weller, J.I. 2004. A complete genome scan of the Israeli Holstein population for quantitative trait loci by a daughter design. J. Dairy Sci. 87 (2): 476-490.
61. Sabour, M.P., Lin,C.Y. and Smith, C.1997. Association of genetic variants of bovine growth hormone with milk production traits in Holstein cattle. J.Anim.Breed.Genet.114:435-442.
62. Sang, B.C., Mazhar, K., Heriz, A., Monteagudo, L.V., Skidmore, C.J. and Arruga, M., 1994. Confirmation of the assignation of the bovine beta-lactoglobulin gene and analysis of polymorphism by the PCR method. ITEA Producing Animal, 90A.3, 155-165, (Abst.).
63. Schlee, P., Graml, R., Rottmann, O. and Pirchner, F. 1994a. Influence of growth hormone genotypes on breeding values of Simmental bulls. J. Anim. Breed. Genet. 111: 253-256.
64. Schlee, P., Graml, R., Schallenberger, E., Schams, D., Rottmann, O., Olbrich B.A. and Pirchner, F. 1994b. Growth hormone and insulin like growth factor I: concentration in bulls of various growth hormone genotypes. Theoretical and Applied Genetics, 88: 497-500.
65. Sejrsen, K., Foldager, J., Sorensen, M.T., Akers, R.M. and Bauman, D.E. 1986. Effect of exogenous bovine somatotropin on pubertal mammary development in heifers. J. Dairy Sci. 69(6):1528-1535.
66. Spelman, R.J., Ford, C.A., McElhinney, P. and Gregory, G.C., 2002. Characterization of the DGAT1 gene in the New Zealand dairy population. J. Dairy Sci. 85: 3514-3517.
67. Strzalkowska, N., Krzyzewski, J., Zwierzchowski, L., Ryniewicz, Z. 2002. Effects of κ-casein and β-lactoglobulin loci polymorphism, cows’age, stage of lactation and somatic cell count on daily milk yield and milk composition in Polish Black-and-White cattle. Animal Science Papers and Reports, 20:21-35.
68. Tambasco, D.D., Paz, C.C.P., Tambasco-Studart, M.D., Pereira, A.P., Alencar, M.M., Freita, A.R., Coutinho, L.L, Packer, I.U. andRegitano, L.C.A. 2003. Candidate genes for growth traits inbeef cattle crosses Bos taurus x Bos indicus. J Anim. Breed. Genet. 120:51-56.
69. Taylor, J. F.; Coutinho, L. L.; Herring, K. L., Gallagher Jr, D. S.; Brenneman, R. A.; Burney, N.; Sanders, J. O.; Turner, J. W.; Smith, S. B.; Miller, R. K.; Savell, J. W.; Davis, S. K., 1998. Candidate Gene Analysis of Gh1 for effects on growth and carcass composition of cattle. Anim. Genet. 29: 194–201.
70. Thaller, G., Kramer, W., Winter, A., Kaupe, B. 2003. Effects of DGAT1 variants on milk production traits in German cattle breeds. J. Anim. Sci. 81: 1911-1918.
71. Unanian, M. M., Barreto, C. C., Freitas, A. R., Cordeiro, C. M. T. and Josahkian, L. A., 2000. Association of growth hormone gene polymorphisms with weight traits in Nelore breed.. Rev. Bras. Zootec. 29: 1380–1386.
72. Van Tassell, C.P., Ashwell, M.S., Sonstegard, T.S., 2000. Detection of putative loci affecting milk, health, and conformation traits in a US Holstein population using 105 microsatellite markers. J Dairy Sci. 83 (8):1865-72.
73. Vlaic, A., Pamfil, D.C., Gaboreanu, I., Vlaic, B. and Renaville, R. 2003. Increasing milk production in cattle using DNA marker assisted selection (Pit-1). Buletinul Universitatii de Stiinte Agricole si Medicina Veterinara Cluj-Napoca, Seria Zootehnie si Biotehnologii 59 : 188-191. (Abst.).
74. Weller, J.I., Golik, M., Seroussi, E. and Ezra E. 2003. Populationwide analysis of a QTL affecting milk-fat production in the Israeli Holstein population. J. Dairy Sci. 86: 2219-2227.
75. Winter, A., Krämer, W., Werner, F.A.O. and Kollers, S., 2002. Association of a lysine-232/alanine polymorphism in a bovine gene encoding acyl-CoA:diacylglycerol acyltransferase (DGAT1) with variation at a quantitative trait locus for milk fat content. Proc. Natl. Acad. Sci. USA 99: 9300-9305.
76. Wu, X.L., MacNeil, M.D., De S. and Xiao, Q.J. 2005. Evaluation of candidate gene effects for beef backfat via Bayesian model selection. Genetica 125: 103-113.
77. Xue, K., Chen, H., Wang, S., Cai, X., Liu, B., Zhang, C-F, Lei, CZ., Wang, X-Z., Wang, Y-M. and Niu, H., 2006. Effect of genetic variations of the POU1F1 gene on growth traits of nanyang cattle. Acta Genetica Sinica, 33 (10) 901-907.
78. Yao, J., Aggrey, S.E., Zadworny D., Hayes, J.F. and Kuhnlein, U. 1996. Sequence variations in the bovine growth hormone gene characterized by single-strand conformation polymorphins (SSCP) analysis and their association with milk production traits in Holsteins. Genetics., 144, 1809– 1816.
79. Zhao, Q., Davis, M.E. and Hines, H.C. 2001. Relationships of polymorphisms in the Pit-1 gene with growth traits in beef cattle. Research and Reviews: Beef and Sheep, Special Circular 181-01.
80. Zhou, G., Zhu, Q., Jin, H.G. and Guo, S.L., 2006a. Genetic variation of growth hormone gene and its relationship with milk production traits in China Holstein cows. AsianAustralasian J. of Anim. Sci. 19 (3) 315-318 (Abst).
81. Zhou G., Zhu, Q., Wu, Y. and Jin, H. 2006b. Polymorphism of PRL gene and its relationship with milk production traits in cows. J. J. Agric.Univ. 28 (1):80-83. (Abst.).
82. Zwierzchowski, L., Krzyzewski, J., Strzalkowska, N., Siadkowska, E. and Ryniewicz, Z. 2002. Effects of polymorphism of growth hormone (GH), Pit-1, and leptin (LEP) genes, cow's age, lactation stage and somatic cell count on milk yield and composition of Polish Black-and-White cows. Animal Science Papers and Reports 20 (4) : 213-227. (Abst.).