Öz

Bu araştırmada fasulye bitkisinin vejetatif organlarının (kök, gövde ve yaprak) soğuğa dayanıklılığı üzerine CaCI2 ve KCI (0.025, 0.05 and 0.1 M)’nin etkileri kontrollü üşüme testleriyle araştırıldı. Fideler, sulama suyuna CaCI2 ve KCI ilave edildikten sonra 1 gün boyunca aklime (150C ışık: 100C karanlık,14 saat ışık: 10 saat karanlık) edildi. Daha sonra fideler 9 gün boyunca üşüme sıcaklıklarına (CTs) (10/5oC) maruz bırakıldı. 0.05 M KCI ve 0.025 M CaCI2 gövde uzamasını teşvik ettiği belirlendi. Nisbi Kuru ağırlık (RDW) genellikle kök ve gövdede tüm CaCI2 ve KCI uygulamaları tarafından azaltıldı, ama RDW bu uygulamalar tarafından yaprakta artırıldı (0.05 M KCI hariç). 0.1 M CaCI2 uygulaması ise RDW’ı bütün organlarda artırdı. 0.1 M CaCI2 uygulamasının her üç organda çözünebilir protein miktarını (SPtC) artırdığı belirlendi. Proline içeriği (PlC) bazı uygulamalar tarafından artırılırken, bazıları tarafından azaltıldı. Gövde canlılığının tüm uygulamalar tarafından azaltılmasına karşın, bazı uygulamalar (kök için 0.025 M KCI, yaprak için 0.025 M KCI, 0.1 and 0.05 M CaCI2 ) tarafından kök ve yaprak canlılığı artırıldı. Biz, bu araştırmada, sağlanabilen direnç cok az olsa bile, belirli KCl ve CaCl2 (0.025 M KCI, 0.025 M KCI, 0.1 ve 0.05 M CaCI2) uygulamalarının üşümeye hassas olan fasulyeye direnç sağlamada kullanılabileceğini önermekteyiz.

Anahtar Kelimeler

• Üşümeye direnç, Canlılık, KCl, CaCl2, Fasulye, Phaseolus vulgaris

Kaynakça

1. Aronson, A., 1980. Frost hardiness in Scots pine (Pinus sylvestris L.) II. Hardiness during winter and spring in young trees of different mineral nutrient status. Stud. For. Suec. 155:1-27.
2. Arora, R. and J.P. Patla, 1986. Protoplasmic swelling as a symptom of freezing injury in onion bulb cells. Its stimulation in extracellular KCI and prevention by calcium. Plant Physiol., 82: 625-629.
3. Arora, R.and J.P. Palta, 1989. Perturbation of membrane calcium as a molecular mechanism of freezing injury, p. 281-290. In, J.H. Cherry (e.d.). Environmental stress in plants. NATO Advanc. Sci. Inst. Series, G 19. Springer–Verlag, Berlin.
4. Bates, L.S. 1973. Rapid determination of free proline for water-stress studies. Plant and soil., 39: 205-207.
5. Bradford, M.M. 1976. A rapid and sensitive method for the quantition of microgram quantities of protein utilising the principle of protein-dye binding analitical. Biochem.,72: 248- 254.
6. Chen, H.H., P.H. Li and M.L. Brenner, 1983. Involvement of abscisic acid in potato cold acclimation. Plant Physiol. 71: 362-365.
7. Christersson, L., 1975. Frost-hardiness development in Pinus silvestris L.seedlings. Stu. For. Sue., 103:1-27.
8. De, B., S. Bhattacharjee and A.K. Mukherjee, 1996. Short therm heat shock and cold shock induced proline accumulation in relation to calcium involvement in Lycopersicon esculentum (Mill) cultured cells and seedlings. Indian J. Plant Physiol., New Series 1(1): 32-35.

9. Dhindsa, R.S.and A.F. Monroy, 1994. Low temperature signal transduction, gene expression, and cold acclimation, Multiple roles of low temperature, p. 501-514. In, J.H. Cherry (e.d.). Biochemical and cellular mechanism of stress tolerance in plants. NATO Advanced Sci. Inst. Series, H 86. Springer- Verlag, Berlin.
10. Duncan, R.D. and J.M. Widholm, 1987. Proline accumulation and its implication in cold tolerance of regenerable maize callus. Plant Physiol., 83: 703-708.
11. Gomez-Lepe, B.E., O.Y. Lee-Stadelmann, J.P. Palta, and E. J.Stadelmann, 1979. Effects of octylguanidine on cell permeability and other protoplasmic properties of Allium cepa epidermal cells. Plant Physiol., 64: 131-138.
12. Gusta, L.V., D.B. Fowler, and N..J. Taylor, 1982. The effect of abscisic acid and cytokinins on the cold hardiness of winter wheat. Can. J.Bot., 60: 301-305.
13. Guy, C.L. and D. Haskell, 1988. Detection of polypeptides associated with the cold acclimation process in spinach. Electrophoresis, 9: 787-796.
14. Knight, H., 2000. Calicium signaling during abiotic stress in plants, Int. Rev. Cytol. 195: 269–324.
15. Kushad, M.M. and G., Yelenosky, 1987. Evaluation of polyamine and proline levels during Low temperature acclimation of citrus. Plant Physiol., 84: 692-95
16. Lalk, I. and K. Dorfling, 1985. Hardening, ABA, proline and freezing resistance in two winter wheat varieties. Physiol. Plant., 63: 287-292.
17. Leonard, R.T.and P.K. Hepler, 1990. Calcium in plant growth and development. Amer. Soc. Plant Physiol., Rockville, Md.
18. Machakova, I., A. Hansiova and J. Krekule, 1989. Levels of ethylene, ACC, MACC, ABA and Proline as an indicator of cold hardening and frost resistance in winter wheat. Physiol. Plant., 76: 603-607.
19. Mohapatra, S.S., R.J.Poole and R.S.Dhindsa, 1988. Abscisic acid- Regulated gene expression in relation to freezing tolerance in Alfalfa1. Plant Physiol., 87: 468-473.
20. Monroy, A.F., F. Sahran and R.S. Dhindsa, 1993. Cold-induced changes in freezing tolerance, protein phosphorilation, and gene expression. Plant Physiol., 102: 1227-1235.
21. Omran, R.G., C.R. Benedict and R.D. Powell, 1971. Effects of chilling on protein synthesis and CO2 fixation on cotton leaves. Crop Science., 11: 554-556.
22. Palta, J.P., J. Lewitt and E.J. Stadelmann, 1977a. Freezing injury in onion bulb cells, I. Evaluation of the conductivity method and analiysis of ion and sugar efflux from injured cells. Plant Physiol., 60: 398-402.
23. Palta, J.P., J. Lewitt and E.J. Stadelmann, 1977b. Freezing injury in onion bulb cells, a comparison of freezing vs. desiccation and living vs. dead cells. Plant Physiol., 41: 273-279.
24. Palta, J.P. and Lee-Stadelmann, E.J., 1983. Vacuolated plant cells as ideal osmometer: Revesibility and limits plasmolsis, and estimation of protoplasm volumme in control and water-stress- tolerant cells. Plant, Cell Environ., 6:601-610.
25. Palta, J.P.and P.H. Li, 1978. In plant cold hardiness and freezing stress-mechanism and crop implications. Edited by P. H. Li and A. Sakai (Acad. press, New York). p.93-115.
26. Palta, J.P. and P.H. Li, 1980. Alterations in membrane transport properties by freezing injury in herbaceous plants. Evidence against rupture theory. Physiol. Plant. 50: 169-175.
27. Palta, J.P., 1996. Role of calcium in plant responses to stresses, Linking basic research to the linking basic research to the solution of practical problems. Hortscience, 31(1): 51-57.
28. Poovaiah, B.W. and A.S.N.Reddy, 1987. Calcium messenger systems in plants. CRC Crit. Rev. Plant Sci., 6, 47-102.
29. Poovaiah, B.W. and A.S.N. Reddy, 1993. Calcium and signal transduction in plants. CRC Crit. Rev. Plant Sci., 12(3), 185- 211.
30. Purvis, A.C., K. Kawada, and W. Grierson, 1979. Relationship between midseason resistance to chilling injury and reducing sugar level in grapefruit peel. HortScience, 14: 227-229.
31. Rigby, P.W.J., M. Dieckmann, C. Rhodes and P. Berg, 1977. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase. I. J. Mol. Biol., 113: 237-251.
32. Sarjala, T., K. Taulavuori, E. Maija and A.B. Edfast, 1997. Does Availability potassium affect cold hardening of Scots pine through polyamine metabolism? Physiol. Plant, 99:56-62.
33. Stadelman, O.Y. and E.J. Stadelman, 1976. In water and plant life, proplems and modern approaches, edited by O L Lange, L Kappen &E D Schulze (Springer- Verlag, Berlin), 268.
34. Steponkus, P.L. and F.O. Lanphear, 1968. The relationship of carbohydrates to cold acclimation of Hedera helix L. cv. Thorndale. Physiol. Plant., 21: 777-91.
35. Suresh, P., Lalitha, K.R., Udaya Kumar, M., Uma Shaanker, R., 1991. Involvement of calcium in abscisic acid, KCL, NaCL and moisture stress induced accumulation of proline in finger millet, Eleusine coracana. Indian J. of Exp.l Biol., 29: 355.
36. Uma Shaanker, R., 1985. Moisture stress induced loss of membrane integrity, accumulation of proline and their inter-relationship, Thesis, Univ. of Agric. Science, Bangalore, India,
37. Wang, C.Y., 1982. Physiological and Biochemical responses of plants to chilling streess. HortScience, 17 (2): 173-185.
38. Wang, L.J. and S.H. Li, 2006. Salicylic acid-induced heat or cold tolerance in relation to Ca2+ homeostasis and antioxidant systems in young grape plants. Plant Science 170: 685–694.
39. Yelenosky, G., 1979. Accumulation of free proline in citrus leaves during cold hardening of young trees in controlled temperature regimes. Plant Physiol., 64: 425-27. .