Buğdayda Olgun Embriyo Kültürünü Etkileyen Faktörler

Öz

Bu çalışma buğdayda olgun embriyo kültürünü etkileyen faktörleri belirlemek amacıyla yapılmıştır. Kallus, embriyogenik kallus, somatik embriyo oluşumu ve rejenerasyon kapasitesine genotipin, jel yapıcı maddenin, oksin tipleri ve dozlarının etkisi çok önemli olmuştur. Buğday olgun embriyo kültüründe oksin tipi olarak dikambanın, 2,4-D ve pikloramdan, yine jel yapıcı madde olarak phytagelin, agardan daha etkiliği olduğu saptanmıştır. En yüksek kallus, embriyogenik kallus ve somatik oluşumu ve de bitki rejenerasyon kapasitesi phytagel ortamındaki dikambanın 4 mg/l dozunda elde edilmiştir. Rejenerasyon ortamının rejenerasyon kapasitesine etkisi çok önemli olmuştur. En yüksek rejenerasyon kapasitesi R1 (0.1 mg/l 2,4-D + 0.5 mg/l BAP) içeren ortamda meydana gelmiştir.

Anahtar Kelimeler

• Genotip, oksin, jel yapıcı madde, rejenerasyon ortamı, Triticum aestivum

Kaynakça

1. Ahmet, H., Adak, M.S., 2007. Irakta yetiştirilen bazı ekmeklik buğday çeşitlerinde kallus oluşumu ve bitki rejenerasyonu. Tarım Bilimleri Dergisi,13(3): 285-292.
2. Babaoğlu, M., Yorgancılar, M., Akbudak, M. A., 2001. Doku kültürü: temel laboratuar teknikleri. Bitki Biyoteknolojisi I, Özcan, S., Gürel, E., Babaoğlu, M., S.Ü. Basımevi, 1-35s, Konya.
3. Bahieldin, A., Dyer, W. E., Qu, R., 2000. Concentration effects of dikamba on shoot regeneration in wheat. Plant Breeding, 119: 437–439.
4. Bı, R.M., Kou M., Chen L.G., Mao S.R., Wang H.G., 2007. Plant regeneration through callus initiation from mature embryo of Triticum. Plant Breeding,126 (9): 9-12.
5. Bohorova, N. E., Pfeiffer, W. H., Mergoum, M., Crossa, J., Pacheco, M., Estanol, P., 2001. Regeneration potential of CIMMYT durum wheat and triticale varieties from immature embryos. Plant Breeding, 120: 291-295.
6. Chen J.Y., Yue R.Q., Xu H.X., Chen X.J., 2006. Study on plant regeneration of wheat mature embryo under endosperm- supported culture. Agric. Sci. China,5: 572-578.
7. Chevreau, E., Mourgues, F., Neveu, M., Chevalier, M.,1997. Effect of gelling agents and antibiotics on adventitious bud regeneration from in vitro leaves of pear. In Vitro Cellular & Developmental Biology, 33 (3): 173-179.
8. Delporte, F., Mostade, O., Jacquemin, J. M., 2001. Plant regeneration through callus initiation from thin mature embryo fragments of wheat. Plant cell, tissue and organ culture, 67: 73-80

9. Elena, B. E., Ginzo, H. D., 1988. Effect of auxin levels on shoot formation with different embryo tissues from a cultivar and a commercial hybrid of wheat (Tritieum aesrivum L.). J. Plant Physiol., 132: 600-603.
10. Fennel, S., Bohorova, N., Ginkel, M., Crossa, J., Hoisington, D. A., 1996. Plant regeneration from immature embryos of 48 elite CIMMYT bread wheats. Theor Appl Genet., 92: 163– 169.
11. Filippov, M., Miroshnichenko D., Vernikovskaya D., Dolgov S., 2006. The effect of auxin and exposure to auxin and genotypes on somatic embryogenesis from mature embryos of wheat. Plant Cell, Tissue and Organ Culture, 84: 213-222. Flehinghaus, T., Deimling, S., Geiger, H. H., 1991. Methodical improvements in rye anter culture. Plant Cell Reports, 10: 397-400.
12. Haliloglu, K., Baenzinger, P. S., 2003a. Agrobacterium tumefaciens mediated wheat transformation. Cereal Research Communications, 31(1-2): 9-16.
13. Haliloglu, K., Baenzinger, P. S., 2003b. The effect of age and size of wheat anther culture-drived embryos on regeneration of green and albino plantlets. Israel journal of plant sciences, 51: 207-212.
14. Haliloglu, K., Baenzinger, P. S., 2005. Screening wheat genotypes for high callus induction and regeneration capability from immature embryos cultures. J. Plant Biochemistry and Biotechnology, 14: 77–82.
15. Haliloglu, K., 2006. Efficient regeneration system from wheat leaf base segments. Biologia Plantarum, 50 (3): 326–330.
16. Hess, J. R., Carman, J. G., 1998. Embriyogenic compotence of immature wheta embryos: genotype, donor plant environment and endogenous hormone levels. Crop Science, 38: 249–253. http://www.agron.missouri.edu/mnl/64/index.html. (25.05.2006). 10
17. plant biology, 3(5): 1-11.
18. Patnaik, D., Vishnudason D., Khurana P., 2006. Agrobacterium- mediated transformation of mature embryos Triticum aestivum and Triticum durum. Current Science, 91(3): 307- 317.
19. Popelka, J. C., Altpeter, F., 2001. Interactions between genotypes and culture media components for improved in vitro response of rye inbred lines. Plant Cell Reports, 20: 575-582.
20. Przetakiewicz, A., Orczyk, W., Nadolska-Orczyk, A., 2003. The effect of auxin on plant regeneration of wheat, barley and triticale.Plant cell. Tissue and organ culture, 73: 245-256.
21. Rashid, H., Ghani, R. A., Chaudhry, Z., 2002. Effect of media, growth regulators and genotypes on callus ınduction and regeneration in wheat (Triticum aestivum).Biotechnology, 1 (1): 49-54.
22. Redway, F. A., Vasil, V., Lu D., Vasil, I. K., 1990. Identification of callus types for long-term maintenance and regeneration from commercial cultivars of wheat (Triticum aestivum L.). Theor Appl Genet, 79: 609–617.
23. Sarker, R.H., Biswas A., 2002. In vitro plantet regeneration and Agrobacterium-mediated genetic transformation of wheat (Triticum aestivum L.). Plant Tissue Cult.,12 (2): 155-165.
24. Satyavathi, V. V., Jauhar, P. P., Elias, E. M., Rao M. B., 2004. Effects of growth regulators on ın vitro plant regeneration in durum wheat. Crop Science, 44: 1830- 1846.
25. Şehirali, S., Özgen M., 1998. Bitki ıslahı, Ankara Üniv. Ziraat Fakültesi
26. Üniv. Basımevi, Ankara, s261.
27. Shah, M.,I., Jabeen, M., ILahi,I., 2003. In vitro callus induction ,its proliferation and regeneration in seed explants of wheat (Triticum aestivum) . Pak.j. Bot., 35(2): 209-217
28. Simonson, R. L., Baenzinger, P. S., 1992. The effect of gelling agents on wheat anter and immature embryo culture. Plant Breeding,109: 211-217.
29. Tremellat, V., Vain,P. and Flament, P., 1990. Effect of gelling agent on callus initiation from immature embryos of inbred A188.
30. Viertel, K., Hess, D., 1996. Shoot tips as an alternative source for regenerable embryogenic callus cultures. Plant Cell Tiss. Organ Cult., 44: 183–188;
31. Yurkova, G. N., Levenko, B. A., Novozhilov, O. V., 1982. Plant regeneration in wheat tissue culture. Biochem Physiol, 177: 337–344.
32. Zale, M. J., Borchardt-Wier, H., Kidwell, K. K., Steber C.M., 2004. Callus induction and plant regeneration from mature embryos of a diverse set of wheat genotypes. Plant cell, Tissue organ culture, 76: 227-281.