Portakalda Antraknoz Hastalığı Etmeni Colletotrichum gloeosporioides’in Biyolojik Mücadele İmkanlarının Araştırılması

Öz

Fungisitlerin insan ve çevre sağlığını olumsuz etkilemesi, hastalık etmenlerinin fungisitlere karşı direnç oluşturması ve tarımsal ürünlerdeki kalıntı sorunu kimyasal mücadeleye alternatif yöntemlerden olan biyolojik mücadelenin önemini bir kat daha artırmıştır. Sebze ve meyvelerde yaygın olarak görülen antraknoz hastalığı etmeninin (Colletotrichum gloesporoides) in vitro koşullarda mikrobiyal antagonistlerle mücadele edilebilirliğinin araştırıldığı bu çalışmada toplam 9 adet biyoajan bakteri izolatı [Bacillus megaterium (TV 3D), Bacillus subtilis (TV 6F, TV 17C, CP 1), Bacillus cereus (TV 85D), Paenibacillus polymxa (TV 12E), Pantoea agglomerans (RK 79, RK 92), Pseudomonas fluorescens (MF 3)] ve 3 adet biyoajan fungus izolatı [Trichoderma harzianum (ET 4, ET 14, NT 1)] kullanılmıştır. Biyoajan fungusların patojene karşı etkililiği petri kaplarına karşılıklı konularak, bakterilerin ise merkeze patojen konulup etrafına bakteri çizilerek yapılmıştır. Uygulama sonucunda tüm mikrobiyal antagonistlerin in vitro şartlarda patojen fungusun gelişimini engellediği ve P. fluorescens’in MF 3 izolatının (%69,05) ve T. harzianum’un ET 4 izolatının (%82,50) en etkili izolatlar olduğu belirlenmiştir. 

Anahtar Kelimeler

• Portakal
• Colletotrichum gloesporoides
• Pseudomonas fluorescens
• Trichoderma harzianum
• Biyolojik mücadele

Investigation of Biological Control Possibility of Anthracnose Disease Agent, Colletotrichum gloeosporioides, on Orange

Abstract

The negative effect of fungicides on human and environmental health, resistance to fungicides and the problem of residue in agricultural products are getting increase the importance of biological control one of the alternative methods for chemical control. Nine bioagent bacterial isolates [Bacillus megaterium (TV 3D), Bacillus subtilis (TV 6F, TV 17C, CP 1), Bacillus cereus (TV 85D), Paenibacillus polymxa (TV 12E), Pantoea agglomerans (RK 79, RK 92), Pseudomonas fluorescens (MF 3)] and three bioagent fungi isolates [Trichoderma harzianum (ET 4, ET 14, NT 1)] were used in this study the investigation of biological control of anthracnose disease (Colletotrichum gloesporoides) common in vegetables and fruits in in vitro. As a result of the application, it was determined that all microbial antagonists prevented the development of pathogen fungus in vitro and P. fluorescens MF 3 isolate (69.05%) and T. harzianum ET 4 isolates (82.50%) were the most effective isolates. 

Keywords

• Orange
• Colletotrichum gloesporoides
• Pseudomonas fluorescens
• Trichoderma harzianum
• Biological control

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