Hyphae Fusion Reactions and Determination of Anastomosis Group by Classical Ways of Rhizoctonia spp. Isolates Obtained from Sugar Beets

Öz

Root rots were detected in 691 of 866 fields from Konya beet sugar beet growing areas between 2015 and 2017 and among these fungi most Rhizoctonia spp. has been identified. In the diagnosis of fungus; morphological diagnostics, isolation, microscopic observation, determinations of anastomosis groups by classical means were used, were used for the definitive diagnosis. 61 isolates of 71 isolates were identified as multinucleate and 10 isolates were identified as binucleate Rhizoctonia spp. In determining anastomosis group with classical method; the isolates are divided into groups, according to hyphal reactions of Rhizoctonia isolates with test isolates; isolates were divided into groups according to C0, C1, C2, C3 type reactions and according to the way the hyphae are directed towards each other. This study is important for leading the studies to be carried out on this subject in terms of visual and methodical detail of anastomosis group determination steps and hyphal fusion in the classical way.

Anahtar Kelimeler

• Anastomosis group
• Beta vulgaris
• Determination of classical AG
• Rhizoctonia spp.

Proje Numarası

18L0447014

Şeker Pancarlarından Elde Edilen Rhizoctonia spp. İzolatlarının Hif Birleşme Reaksiyonları ve Klasik Yollarla Anastomosis Grup Temini

Öz

2015-2017 yılları arasında Konya bölgesi şeker pancarı ekim alanlarından 866 tarlanın 691’inde kök çürüklükleri tespit edilmiş ve elde edilen birçok fungus içerisinden en çok Rhizoctonia spp. elde edilmiştir. Fungusun tanısında; morfolojik tanılama, izolasyon, mikroskobik gözlem, klasik yollarla anastomosis grupların tespitleri kullanılmıştır. Elde edilen 71 Rhizoctonia izolatlarından, 61 izolat multinükleat Rhizoctonia solani, 10 izolatta binükleat Rhizoctonia spp. olarak tanımlanmıştır. Klasik metotla anastomosis grubu belirlemede test izolatlarla, elde edilen Rhizoctonia izolatlarının hifsel reaksiyonlarına bakılarak C0, C1, C2, C3 tipi reaksiyonları ve hiflerin birbirine doğru yönelme şekillerine göre de anastomosis grupları tespit edilmiştir. Bu çalışma klasik yolla anastomosis grup belirleme aşamalarını ve hifsel kaynaşmayı görsel ve metotsal olarak detaylandırarak, bu konuda yapılacak olan çalışmalara bir ışık tutması açısından önem taşımaktadır.

Anahtar Kelimeler

• Anastomosis grup
• Beta vulgaris
• Klasik AG tespiti
• Rhizoctonia spp.

Destekleyen Kurum

Ankara Üniversitesi BAP Projesi

Proje Numarası

18L0447014

Kaynakça

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