Monitoring Nutrient Uptake of Chard (Beta vulgaris var. cicla L.) Exposed to Exogenously Applied Nitric Oxide under Drought Stress

Abstract

Nutrient uptake of chard under well-watered and drought conditions with different NO applications were investigated in this research. NO solutions were prepared with four doses (0, 50, 100, 150 and 200 µM) of sodium nitroprusside as a nitric oxide source and exogenously applied on only seeds (s) or together with seed and foliar (sf) of chard under different levels of drought according to 100% (control), 67% and 33% of the water required to reach field capacity. Nutrient uptake drastically decreased under drought stress. However, progressive effects of NO applications on nutrient uptake have been observed under both well-watered conditions and under drought. The increase in N and P uptake of plant were significant especially for doses of 100 and 150 µM for both s and sf applications. The highest Fe, Cu and Zn content were obtained from 150 µM sf NO application, while the highest Mn and B were obtained from 150 µM s and 200 µM sf NO application in 67% level. However, in 33% level the highest Cu and B content were obtained from 150 µM sf NO application, while the highest Mn and Zn obtained from 100 µM sf and the highest Fe obtained from the 200 µM s NO application.

Keywords

• Water deficit
• Plant nutrients
• Nitric oxide

Kuraklık Stresi Altında Dışarıdan Nitrik Oksit Uygulanan Pazıda (Beta vulgaris var. cicla L.) Besin Alımının İzlenmesi

Öz

Bu araştırmada farklı NO uygulamaları altında tam sulanmış ve kurak koşullarda yetiştirilmiş pazı bitkisinin besin maddesi alımı incelenmiştir. Uygulamalar, bitki kök bölgesinde tarla kapasitesine ulaşmak için gerekli suyun %100 (kontrol), %67 ve %33'ünün sağlandığı kuraklık seviyelerinde, nitrik oksit donörü olarak dört doz (0, 50, 100, 150 ve 200 µM) sodyum nitroprussid ile NO çözeltilerinin kullanıldığı çözeltilerle sadece tohum (s) veya tohum ve yapraklara (sf) olmak üzere dışarıdan uygulanmıştır. Kuraklık stresi altında besin alımı önemli ölçüde azalmıştır. Bununla birlikte, NO uygulamalarının besin alımı üzerindeki geliştirici etkileri hem tam sulanan koşullar hem de kurak koşullar altında gözlemlenmiştir. Bitkinin N ve P alımındaki artış, özellikle hem s hem de sf uygulamaları için 100 ve 150 µM'lik dozlarda önemli olmuştur. % 67 sulama seviyesinde en yüksek Fe, Cu ve Zn içeriği 150 µM sf NO uygulamasında olurken, en yüksek Mn ve B ise 150 µM s ve 200 µM sf NO uygulamasından elde edilmiştir. Ancak %33 seviyesinde en yüksek Cu ve B içeriği 150 µM sf NO uygulamasından elde edilirken, en yüksek Mn ve Zn 100 µM sf'den, en yüksek Fe ise 200 µM s NO uygulamasından elde edilmiştir.

Anahtar Kelimeler

• Su kısıtı
• Bitki besinleri
• Nitrik oksit

Kaynakça

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