Çimlenen Fasulye Tohumlarının Antioksidan Enzim Aktiviteleri, H2O2 İçeriği ve Lipid Peroksidasyonu Üzerine Memeli Cinsiyet Hormonlarının Etkileri

Yıl 2009, Cilt: 40 Sayı: 2, 79 - 85, 11.02.2013

Serkan Erdal

Öz

Bu çalışmada, çimlenen fasulye tohumlarında süperoksit dismutaz, peroksidaz ve katalaz enzim aktiviteleri ile hidrojen
peroksit (H2O2) içeriği ve lipid peroksidasyon seviyesi üzerine memeli cinsiyet hormonları olan progesteron, , β-östradiol ve
androsteronun etkileri araştırıldı. Fasulye tohumları 10-4 ile 10-15 M konsantrasyon aralığında değişen hormon çözeltilerinde
çimlendirildi. Uygulanan tüm konsantrasyonlarda süperoksit dismutaz, peroksidaz ve katalaz enzim aktiviteleri kendi kontrollerine
oranla önemli derecede arttı. Maksimum enzim aktiviteleri her üç hormon içinde 10-9 M konsantrasyonda kaydedildi. Diğer yandan,
hormon uygulanan tohumlarda antioksidan enzim aktivitelerindeki artışa bağlı olarak lipid peroksidayon seviyesi ve hidrojen
peroksit içeriği önemli ölçüde azaldı.

Anahtar Kelimeler

Memeli cinsiyet hormonları, Çimlenme, Antioksidan enzimler, Stres parametreleri

Kaynakça

• Agarwal, S. and V. Pandey, 2004. Antioxidant enzyme responses to NaCl stress in Cassia angustifoli. Biol. Plant. 48: 555-560.
• Aisien, A.O., G.H. Palmer and J.R. Stark, 1983. The development of enzymes during germination and seedling growth in Nigerian sorghum. Staerke, 35: 316-320.
• Apel, K. and H. Hirt, 2004. Reactive oxygen species: Metabolism, oxidative stress, and signal transduction.  Annu. Rev. Plant Biol. 55: 373-399.
• Blokhina, O., E. Virolainen and K.V. Fagerstedt, 2003. Antioxidants, oxidative damage and oxygen deprivation stress: a review. Ann. Bot. 91: 179-194.
• Bonner, J. and G. Axtman, 1937. The growth of plant embryos invitro. Preliminary experiments on the role of accessory substances. The Proceedings of the National Academy of Sciences, 23: 453-457.
• Dogra, R. and A. Kaur, 1994. Effect of steroids on some growth and biochemical parameters of Triticum aestivum L. during germination. Crop. Res. 8: 611-620.
• Dogra, R. and A. Thukral, 1991. Effect of steroid and plant hormones on some germination aspects of Triticum aestivum L. In: New Trends in Plant Physiology (Dhir K. K., Dua I. S., and Chark K. S.). Today and Tomorrow's Printers and Publishers, New Delhi, pp. 65-70.
• Dohrn, M., W. Faure, H. Poll and W. Blotevogel, 1926. Tokokinine, Stoffe mit sexualhormonartiger Wirkung aus Pflanzenzellen. Med. Klin. 22: 1417-1419.
• Foyer, C.H., P. Descourvieres and K.J. Kunert, 1994. Protection against oxygen radicals-an important defense-mechanism studied in transgenic plants. Plant Cell Environ. 5: 507-523.
• Gong, Y., P.M.A. Toivonen, O.L. Lau and P.A. Wiersma, 2001. Antioxidant system level in ‘Braeburn’ apple is related to its browning disorder. Bot. Bull. Acad. Sin. 42: 259-264.
• He, Y., Y. Liu, W. Cao, M. Huai, B. Xu and B. Huang, 2005. Effects of salicylic acid on heat tolerance associated with antioxidant metabolism in Kentucky bluegrass. Crop Sci. 45: 988–995.
• Helmkamp, G. and J. Bonner, 1952. Some relationships of sterols to plant growth. Plant Physiol. 28: 428-436.
• Janda, T., G. Szalai, K. Rios-Gonzales, O. Veisa and E. Paldi, 2003. Comparative study of frost tolerance and antioxidant activity in cereals. Plant Sci. 164: 301–306.
• Janeczko, A. and A. Skoczowski, 2005. Mammalian sex hormones in plants. Folia Histochem Cyto. 43: 71-79.
• Kato-Noguchi, H. and F.M. Macias, 2005. Effects of 6-methoxy-2- benzoxazolinone on the germination and α-amylase activity in lettuce seeds. J. Plant Physiol. 162: 1304-1307.
• Kocacaliskan, I., 2004. Bitki Fizyolojisi. Kütahya, Turkiye, 154- 155. [in Turkish]
• Kopcewicz, J., 1970. Influence of estrogens on the flower formation in Cichorium intybus L. Naturwissenschaften, 57: 136-137.
• Kögl, F. and A.J. Haagen-Smit, 1936. Biotin und Aneurin als Phytohormone.Z. Physiol. Chem. 243: 209-227.
• Marero, L.M., E.M. Payumo, E.C. Librando, W.N. Lainez, M.D. Gopez and S. Homma, 1988. Technology of weaning food formulations prepared from germinated cereals and legumes. J. Food Sci. 9: 17-33.
• Martínez-Honduvilla, C.J., A. Gimenez-Solves and A. Santos- Ruiz, 1976. Biochemical changes in Pinus pinea seeds. III. The effect of growth substances and steroidal hormones on nucleic acids. Rev. Esp. Fisiol. 32: 169-85.
• Mittler, R., S. Vanderauwera, M .Gollery, F. Van Breusegem, 2004. Recative oxygen gene network of plants. Trends Plant Sci. 9: 490-498.
• Singh, S., S. Eapen and S.F. D’Souza, 2006. Cadmium accumulation and its influence on lipid peroxidation and antioxidative system in an aquatic plant, Bacopa monnieri L. Chemosphere, 62: 233–246.
• Velikova, V., I. Yordanov and A. Edreva, 2000. Oxidative stress and some antioxidant systems in acid rain-treated bean plants. Protective role of exogenous polyamines. Plant Sci. 151: 59–66.
• Verma, S. and S.M. Mishra, 2005. Putrescine alleviation of growth in salt stressed Brassica juncea by inducing antioxidative defense system. J. Plant Physiol. 162: 669-677.