Yaşın Tükürük Transforme Edici Büyüme Faktör Beta (TGF-β) Düzeyleri Üzerindeki Etkisi

Öz

Amaç: Transforme edici büyüme faktör-beta (TGF-β), birçok hücrede proliferasyon, hücresel farklılaşma, motilite ve adezyon gibi fonksiyonları kontrol eden bir sitokin türüdür. Ayrıca, organizmada tüm dokuların gelişiminde, homeostazisinde ve onarımında çok önemli rol oynarlar. Bu çalışmanın amacı, belirlenen yaş gruplarında TGF-β düzeylerinin tükürük örneklerinde değişiminin değerlendirilmesidir. Gereç ve Yöntem: Çalışmaya 26-75 yaş aralığında 32 erişkin (15 kadın/ 17 erkek) birey dahil edildi. Stimüle edilmiş tükürük örnekleri, sabah 08.00-09.00 saatleri arasında SARSTEDT marka tükürük toplama tüpleri ile önerilen şekilde tüp kartuşlarının 2 dakika ağızda çiğnetilmesi ile toplandı. Sonrasında 30 dk bekleme ve 3200 g ‘de 15 dakika santrüfüj yapıldı. Tükürük TGF-β düzeyleri ELISA kiti (pg/mL) ile çalışıldı. Bulgular: Tükürük TGF-β düzeyleri belirlenen yaş grupları arasında anlamlı olarak yüksek bulundu (p=0.0001). Sonuç: Sonuç olarak, 55 yaş üstü bireylerde tükürük TGF-β düzeylerinde gözlenen artışın aynı yaş grubu serum düzeyleri ile yapılan çalışmalarla benzer sonuçlar göstermesi tükürük örneklerinin kullanılabilirliğine dikkat çekmektedir.

Anahtar Kelimeler

• Tükürük
• Sitokin
• Transforme edici büyüme faktör-beta
• Yaş

Effect of Age on Salivary Transforming Growth Factor Beta (TGF-β) Levels

Öz

Objective: Transforming growth factor-beta (TGF-β) is a type of cytokine that controls functions such as proliferation, cellular differentiation, motility, and adhesion in many cells. They also play a very important role in the development, homeostasis and repair of all tissues in the organism. The aim of this study is to evaluate the changes in TGF-β levels in the determined age groups. Materials and methods: 32 adult (15 female / 17 male) individuals aged between 26-75 years were included in the study. Stimulated saliva samples were collected between 08.00-09.00 in the morning by chewing the tube cartridges in the mouth for 2 minutes with SARSTEDT brand saliva collection tubes as recommended. Afterwards, it was waited for 30 minutes and centrifuged at 3200 g for 15 minutes. Saliva TGF-β levels (pg/mL) were studied with the ELISA kit. Results: Salivary TGF-β levels were found to be significantly higher among the specified age groups (p=0.0001). Conclusion: In conclusion, the increase observed in salivary TGF-β levels in individuals over 55 years of age showed similar results with studies conducted with serum levels in the same age group, which draws attention to the usability of saliva samples.

Anahtar Kelimeler

• Saliva
• Cytokine
• Transforming growth factor-beta
• Age

Kaynakça

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