Kanser Hastalarında Fertilitenin Korunmasında Kullanılan Kriyoprezervasyon Yöntemleri

Yıl 2018, Cilt: 1 Sayı: 2, 17 - 26, 15.10.2018

Gülnaz Kervancıoğlu

Öz

Günümüzde erken tanı ve tedavilerdeki gelişmelerle kanser hastalarında sağ kalım süreleri

uzamıştır. Kanserli hastanın onkoloji tedavisinden sonra da üreme yeteneğinin korunması

yaşam kalitesinin yükseltilmesinde önemli bir yer tutmaktadır. Erişkin kadın ve erkeklerde

olgun üreme hücrelerine ulaşmak mümkündür. Embriyo ve oosit dondurulmasında vitrifikasyon

yöntemi, spermatozoa ve testis hücre süspansiyonu dondurulmasında yavaş dondurma yöntemi,

over dokusu dondurulmasında ise her iki yöntem tercih edilmektedir. Prepubertal dönemde

ise gonad dokularının yani üreme kök hücrelerinin kriyoprezervasyonu tek seçenek olarak

karşımıza çıkmaktadır. Ancak prepubertal testis dokusunun kriyoprezervasyonu henüz deneysel

aşamadadır. Kanser hastaları için fertilite restorasyon tekniklerindeki gelişmeler, yakın bir

gelecekte kliniklerde rutin olarak uygulanabilmesi açısından umut vadetmektedir. Ancak

prepubertal erkek çocuklarda testis dokusundaki spermatogonyal kök hücrelerin güvenli ve en

uygun şekilde kriyoprezervasyonunu sağlayacak detaylı araştırmalara ihtiyaç vardır.

Anahtar Kelimeler

Kriyoprezervasyon, Fertilite prezervasyonu, Prepubertal testis, Kanserde kriyoprezervasyon

Kaynakça

• 1. Jemal A, Siegel R, Ward E, et al. Cancer statistics. CA Cancer J Clin 2009; 59(4): 225-249. 2. Wallace WHB, Thomson AB. Preservation of fertility in children treated for cancer. Arch Dis Child 2003; 88: 493-496. 3. Isachenko V, Lapidus I, Isachenko E, et al. Human ovarian tissue vitrification versus conventional freezing: morphological, endocrinological, and molecular biological evaluation. Reproduction 2009; 138: 319-327. 4. Keros V, Rosenlund B, Hultenby K, et al. Optimizing cryopreservation of human testicular tissue: comparison of protocols with glycerol, propanediol and dimethylsulphoxide as cryoprotectants. Hum Reprod 2005; 20: 1676-1687. 5. KerosV, Hulten K, Borgstro B et al. Methods of cryopreservation of testicular tissue with viable spermatogonia in pre-pubertal boys undergoing gonadotoxic cancer treatment. Hum Reprod 2007; 22: 1384-395. 6. Delilbaşı L. In Vitro Fertilizasyon (IVF). Laboratuvar yöntemleri (Yeni uygulamalar ve güncel yaklaşımlar). Ankara: Güneş Kitabevleri; 2008. 61-83. 7. Curry MR. Cryopreservation of Semen from Domestic Livestock. Ed: Day JG, McLellan MR, Cryopreservation and Freeze-Drying Protocols. Totowa:Humana Press; 1995. 189-197. 8. Bunge RG, Keettel WC, Sherman JK. Clinical use of frozen semen. Fertil Steril 1954; 5: 520–529. 9. Mahadevan MM, Trounson AO, LeetonJF. Successful use of human semen crybanking for in vitro fertilization. Fertil Steril 1983; 40(3): 340- 343. 10. Wittingham DG, Leibo SP, Mazur P.Survival of mouse embryo frozen to196°C and -269°C. Science 1972; 178: 411-414. 11. Zeilmaker GH, Alberta AT, Gent VanI, et al. Two pregnancies following transfer of intact frozenthawed embryos. Fertil Steril 1984; 42: 293-296. 12. Rall WF, Fahy GM ‘’Ice-free cryopreservation of mouse embriyos at -196 degrees C by vitrification’’. Nature 1985; 313 (6003): 573-575. 13. Avarbock MR, Brinster CJ, Brinster RL. Reconstitution of spermatogenesis from frozen spermatogonial stem cells. Nat Med1996; 2(6): 693-696. 14. Zeng W, Snedaker AK, Megee S. Preservation and transplantation of porcine testis tissue. Reprod Fertil Dev 2009; 21(3): 489-497. 15. Mature oocyte cryopreservation: a guideline. Practice Committees of American Society for Reproductive Medicine, Society for Assisted Reproductive Technology. Fertil Steril 2013; 99: 37-43. 16. Sheikhi M, Hultenby K, Niklasson B et al. Clinical grade vitrification of human ovarian tissue: an ultrastructural analysis of follicles and stroma in vitrified tissue. Hum Reprod 2011; 26: 594-603. 17. Junqueira LC, Carneiro J. Çev: Aytekin Y, Solakoğlu S. Temel histoloji. 10. Baskı. İstanbul: Nobel Tıp Kitabevleri; 2006. 449-467. 18. Ting AY, Yeoman RR, Lawson MS, et al. In vitro development of secondary follicles from cryopreserved rhesus macaque ovarian tissue after slow-rate freeze or vitrification. Hum Reprod 2011; 26: 2461-2472. 19. Youm HW, Lee JR, Lee J, et al. Optimal vitrification protocol for mouse ovarian tissue cryopreservation: effect of cryoprotective agents and in vitro culture on vitrified-warmed ovarian tissue survival. Hum Reprod 2014; 29: 720-730. 20. Ting AY, Yeoman RR, Campos JR, et al. Morphological and functional preservation of preantral follicles after vitrification of macaque ovarian tissue in a closed system. Hum Reprod 2013; 28: 1267-1279. 21. So-Youn Kim, Seul Ki Kim, Jung Ryeol Lee et al. Toward precision medicine for preserving fertility in cancer patients: existing and emerging fertility preservation options for women.J Gynecol Oncol 2016; 27(2):e22. 22. Kanatsu-Shinohara M, Ogonuki N, Inoue K et al. Restoration of fertility in infertile mice by transplantation of cryopreserved male germline stemcells. Hum Reprod 2003; 18: 2660-2667. 23. Izadyar F, Matthijs-Rijsenbilt JJ, den Ouden K et al. Development of a cryopreservation protocol for type a spermatogonia. J Androl 2002; 23: 537- 540. 24. Moore KL, Persaud TVN. Çev: Dalçık H, Yıldırım M. Klinik yönleriyle insan embriyolojisi, İstanbul: Nobel Tıp Kitabevleri; 2009, 244-283. 25. Tam PP, Snow MH. Proliferation and migration of primordial germ cells during compensatory growth in mouse embryos. J Embryol Exp Morphol 1981; 64: 133-147. 26. McLaren A. Primordial germ cells in the mouse. Dev Biol 2003; 262(1): 1-15. 27. Töhönen V, Ritzén EM, Nordqvist K et al. Male sex determination and prenatal differentiation of the testis. Endocr Dev 2003; 5: 1-23. 28. de Rooij DG and Anton GJ. Spermatogonial stem cells. Current Opinion in Cell Biology 1998; 10(6): 694-701. 29. de Rooij DG, Russell LD. All you wanted to know about spermatogonia but were afraid to ask. J Androl 2000; 21(6): 776-798. 30. Drumond AL, Meistrich ML, Chiarini-Garcia H. Spermatogonial morphology and kinetics during testis development in mice: a high-resolution light microscopy approach. Reproduction 2011; 142: 145-155. 31. Nagano R, Tabata S, Nakanishi Yet al. Reproliferation and relocation of mouse male germ cells (gonocytes) during prespermatogenesis. Anat Rec 2000; 258: 210-220. 32. McGuinness MP, Orth JM. Reinitiation of gonocyte mitosis and movement of gonocytes to the basement membrane in testes of newborn rats in vivo and in vitro. Anat Rec 1992; 233: 527-537. 33. Oakberg EF. A new concept of spermatogonial stem-cell renewal in the mouse and its relationship to genetic effects. Mutat Res 1971; 11(1): 1-7. 34. Ross MH, Pawlina W. Male reproductive system. Histology: A Text and Atlas. 6th ed. China: 2011. 784-828. 35. Tegelenbosch RA, de Rooij DG. A quantitative study of spermatogonial multiplication and stem cell renewal in the C3H/101 F1 hybrid mouse. Mutat Res 1993; 290:193-200. 36. Cheng CY, Mruk DD. Cell junction dynamics in the testis: Sertoli germ cell interactions and male contraceptive development. Physiol Rev 2002; 82(4): 825-874. 37. Russell LD, Ettlin RA, Hikim APSet al. Histopathological evaluation of the testis. 1st ed. Cache River Press; 1990.1-58. 38. Ji C-Y, Ohsawa S. Onset of the release of spermatozoa (spermarche) in Chinese maleyouth. Am J Hum Biol 2000; 12: 577-587. 39. Schlatt S, von Schonfeldt V, Schepers AG. Male germ cell transplantation: an experimental approach with a clinical perspective. Br Med Bull 2000; 56: 824-836. 40. Kervancıoğlu G, Çetinel Ş, Demirci EK at al.The effects of a seeding process on the cryopreservation of cellular suspensions of prepubertal testis. Marmara Medical Journal 2015; 28: 13-20. 41. Griswold MD. The central role of Sertoli cells in spermatogenesis. Cell Dev Biol 1998; 9: 411-416. 42. Nugent D, Meirow D, Brook PF et al. Transplantation in reproductive medicine: previous experience, present knowledge and future prospects. Hum Reprod Update 1997; 3: 267-280. 43. Crabbe E, Verheyen G, Tournaye Het al. Freezing of testicular tissue as a minced suspension preserves sperm quality better than whole-biopsy freezing when glycerol is used as cryoprotectant. Int J Androl 1999; 22: 43-48. 44. Mazur P, Leibo SP, Chu EHY. A two-factor hypothesis of freezing injury evidence from Chinese hamster tissue culture cells. Exp Cell Res 1972; 71: 345-355. 45. Mazur P, Rall WF, Leibo SP. Kinetics of water loss and the likelihood of intracellular freezing in mouse ova. Influence of the method of calculating the temperature dependence of water permeability. Cell Biophys 1984; 6: 197-213. 46. Benson JD, Woods EJ, Walters EM et al. The cryobiology of spermatozoa. Theriogenology 2012; 78: 1682-1699. 47. Hossain A, Nagamani M. Erkek üreme hücrelerinin dondurularak saklanması. Çev: Kervancıoğlu G, Kervancıoğlu E. İnfertilite ve yardımla üreme teknikleri. Ankara: Güneş Kitabevi; 2012. 466-477. 48. Keros V, Rosenlund B, Hultenby K, et al. Optimizing cryopreservation of human testicular tissue: comparison of protocols with glycerol, propanediol and dimethylsulphoxide as cryoprotectants. Hum Reprod 2005; 20: 1676-1687. 49. Wyns C, Curaba M, Martinez-Madrid B et al. Spermatogonial survival after cryopreservation and shortterm orthotopic immature human cryptorchid testicular tissue grafting to immunodeficient mice. Hum Reprod 2007; 22: 1603-1611. 50. Brockbank KGM, Song YC, Khirabadi BS. Storage of Tissues by Vitrification. Transplantation Proceedings 2000; 32: 3-4. 51. Morris GJ, Acton E. Controlled ice nucleation in cryopreservation. Cryobiology 2013; 66(2):85- 92. 52. Whittingham DG. Some factors affecting embryo storage in laboratory animals, Ed: Elliot K, Whelan J, The Freezing of Mammalian Embryos. Amsterdam: Ciba Foundation 52; 1977. 97-127. 53. Fahy GM, Wowk B, Pagotan Ret al. Physical and biological aspects of renal vitrification. Organogenesis 2009; 5 (3): 167-175. 54. Fahy GM, Saur J, Williams RJ. Physical problems with the vitrification of large biological systems. Cryobiology 1990; 27(5): 492-510.