Levels of Genes Encoded in Type 2 Diabetes Model Rat Liver Tissues

Öz

Aim: Obesity and type 2 diabetes are a multifactorial condition caused by environmental and genetic factors.In this study, we aimed to do examine the mRNA levels of HNF4A, LMNA, WFS1, ADAMTS9 genes, which were identified as candidate genes for type 2 diabetes and obesity in previous studies and expressed in liver tissue, in rats created with a type 2 diabetes model with high fat diet and Streptozotocin (STZ). Subject and Method: Rats were given with high-fat diet (involved %50 fat) for 20 weeks received of STZ (40mg/ml) single dose with intraperitoneal injection and insulin tolerance test were carried out. All of rats were sacrificed at the end of 16 weeks and livers were removed for molecular analysis. Real Time PCR was performed to compare the expression levels ofHNF4A, LMNA, WSF1 and ADAMTS9 genes in groups. Results: According to the results of to the study, HNF4A, LMNA, WFS1, ADAMTS9 mRNA expression levels were found to be lower in type 2 diabetes and obesity model rats compared to the control group. Conclusion: This study, which contributes to the understanding of the molecular mechanisms
underlying the pathophysiology of type 2 diabetes and obesity, points out that HNF4A, LMNA, WSF1, ADAMTS9
can be evaluated as potential new biomarkers in the therapeutic approach.

Anahtar Kelimeler

• Experimental
• gene expression profiling
• obesity
• tip 2 diabetes

Proje Numarası

TSA-2017-9090 no’lu proje

Tip 2 Diyabet Modeli Ratların Karaciğer Dokularında Kodlanan Genlerin İfade Düzeyleri

Öz

Amaç: Obezite ve tip 2 diyabet çevresel ve genetik faktörlerin bir araya gelerek meydana getirdiği multifaktöriyel bir durumdur. Yaptığımız çalışmada yüksek yağlı diyet ve Streptozotosin (STZ) ile tip 2 diyabet modeli oluşturduğumuz ratlarda, daha önce yapılan çalışmalarda tip 2 diyabet ve obezite için aday gen olarak belirlenen ve karaciğer dokusunda eksprese olan, HNF4A, LMNA, WFS1, ADAMTS9 genlerinin mRNA düzeylerini incelemeyi hedefledik. Gereç ve Yöntem: Ratlara, 20 hafta yüksek yağlı diyet (%50 iç yağı ), tek doz intraperitonal STZ enjeksiyonu, insülin tolerans testi uyguladık. Tüm ratlar 20. Hafta sonunda sakrifiye edildi ve moleküler analizler için karaciğer dokusu izole edildi. Real-time PCR ile tüm gruplarda HNF4A, LMNA, WFS1, ADAMTS9 genlerinin ekspresyon seviyeleri karşılaştırıldı. Bulgular: Çalışma sonuçlarımıza göre HNF4A, LMNA, WFS1, ADAMTS9 mRNA ekspresyon seviyeleri tip 2 diyabet ve obezite modeli ratlarda kontrol grubuna kıyasla daha düşük bulunmuştur. Sonuç: Tip 2 diyabet ve obezite patofizyolojisinin altında yatan moleküler mekanizmaların anlaşılmasına katkıda bulunan çalışmamız, terapötik yaklaşımda, potansiyel yeni biyomarkırlar olarak HNF4A, LMNA, WSF1, ADAMTS9’un değerlendirilebileceğine işaret etmektedir.

Anahtar Kelimeler

• Diabetes mellitus
• deneysel
• obezite
• gen ekspresyon profili

Destekleyen Kurum

Çalışmamız Çukurova Üniversitesi Bilimsel Araştırma Projeleri Birimince TSA-2017-9090 no’lu proje olarak desteklenmiştir.

Proje Numarası

TSA-2017-9090 no’lu proje

Teşekkür

Çalışmamız Çukurova Üniversitesi Bilimsel Araştırma Projeleri Birimince TSA-2017-9090 no’lu proje olarak desteklenmiştir. Bilimsel Araştırma Projeleri Birimine, desteklediklerinden dolayı teşekkür ederiz.

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