Wound healing activities of eucalyptol on full thickness excisional skin wound model in rats

Mehmet Zeki Yılmaz Deveci; Ömer Kırgız; Hüseyin Özkan; Cafer Tayer İşler; Filiz Kazak Akçakavak; Ziya Yurtal; Akın Yakan

doi:10.33988/auvfd.1677537

Wound healing activities of eucalyptol on full thickness excisional skin wound model in rats

Abstract

A plant-derived monoterpene, eucalyptol (1,8-cineole), has been suggested to have antioxidant, anti-inflammatory, and antimicrobial properties. The wound-healing effects of eucalyptol remain unknown. This study aimed to evaluate the topical use of eucalyptol at two concentrations on a full-thickness excisional skin wound model in terms of wound healing, gene expression, biochemical changes, and histopathological changes. Sixty Wistar rats were randomly divided into 5 groups (n = 12 per group). The control group (C) was untreated. Full-thickness excisional skin wounds were created in the following groups: the vehicle group (V), which was treated with polysorbate 80 solution (negative control). The Dexpanthenol group (D) was treated with dexpanthenol ointment (positive control). Eucalyptol 5% (E5) and Eucalyptol 10% (E10) groups were treated with 5% and 10% eucalyptol, respectively. Wound areas were measured on days 1, 4, 7, 11, 14, 18, and 21 days after wound creation. Wound tissues were collected on days 7 and 21. Histopathological, gene expression (TNFα, IL10, TGFβ1, VEGF), and biochemical (MDA, rGSH, GPx, CAT) analyses were performed on the wound tissues. TNFα gene expression levels were upregulated in Groups V and E5 (P<0.05). IL10 levels were upregulated in the V, E5, and E10 groups (P<0.05). TGFβ1 was upregulated in all groups compared with the control. Significant differences in rGSH, GPx, CAT, and MDA levels were found among all groups (P<0.05). Topical administration of 10% eucalyptol accelerates wound healing. With respect to epidermal thickness, 5% eucalyptol resulted in superior wound healing. Further studies should include different wound models, tissue analyses, and combinations of eucalyptol with other agents.

Keywords

Project Number

20.M.051

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Details

Primary Language

English

Subjects

Veterinary Biochemistry, Veterinary Surgery, Animal Science, Genetics and Biostatistics

Journal Section

Research Article

Authors

Mehmet Zeki Yılmaz Deveci ^*
0000-0002-9532-247X
Türkiye

Ömer Kırgız
0000-0002-0222-1363
Türkiye

Hüseyin Özkan
0000-0001-5753-8985
Türkiye

Cafer Tayer İşler
0000-0002-1910-8316
Türkiye

Filiz Kazak Akçakavak
0000-0002-9065-394X
Türkiye

Ziya Yurtal
0000-0001-6080-1860
Türkiye

Akın Yakan
0000-0002-9248-828X
Türkiye

Early Pub Date

November 28, 2025

Publication Date

January 1, 2026

Submission Date

May 1, 2025

Acceptance Date

November 9, 2025

Published in Issue

Year 2026 Volume: 73 Number: 1

DOI

https://doi.org/10.33988/auvfd.1677537

IZ

https://izlik.org/JA86MX52WX

Cite

RIS / Bibtex

APA

Deveci, M. Z. Y., Kırgız, Ö., Özkan, H., İşler, C. T., Kazak Akçakavak, F., Yurtal, Z., & Yakan, A. (2026). Wound healing activities of eucalyptol on full thickness excisional skin wound model in rats. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 73(1), 83-92. https://doi.org/10.33988/auvfd.1677537

AMA

1.Deveci MZY, Kırgız Ö, Özkan H, et al. Wound healing activities of eucalyptol on full thickness excisional skin wound model in rats. Ankara Univ Vet Fak Derg. 2026;73(1):83-92. doi:10.33988/auvfd.1677537

Chicago

Deveci, Mehmet Zeki Yılmaz, Ömer Kırgız, Hüseyin Özkan, et al. 2026. “Wound Healing Activities of Eucalyptol on Full Thickness Excisional Skin Wound Model in Rats”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 73 (1): 83-92. https://doi.org/10.33988/auvfd.1677537.

EndNote

Deveci MZY, Kırgız Ö, Özkan H, İşler CT, Kazak Akçakavak F, Yurtal Z, Yakan A (January 1, 2026) Wound healing activities of eucalyptol on full thickness excisional skin wound model in rats. Ankara Üniversitesi Veteriner Fakültesi Dergisi 73 1 83–92.

IEEE

[1]M. Z. Y. Deveci et al., “Wound healing activities of eucalyptol on full thickness excisional skin wound model in rats”, Ankara Univ Vet Fak Derg, vol. 73, no. 1, pp. 83–92, Jan. 2026, doi: 10.33988/auvfd.1677537.

ISNAD

Deveci, Mehmet Zeki Yılmaz - Kırgız, Ömer - Özkan, Hüseyin - İşler, Cafer Tayer - Kazak Akçakavak, Filiz - Yurtal, Ziya - Yakan, Akın. “Wound Healing Activities of Eucalyptol on Full Thickness Excisional Skin Wound Model in Rats”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 73/1 (January 1, 2026): 83-92. https://doi.org/10.33988/auvfd.1677537.

JAMA

1.Deveci MZY, Kırgız Ö, Özkan H, İşler CT, Kazak Akçakavak F, Yurtal Z, Yakan A. Wound healing activities of eucalyptol on full thickness excisional skin wound model in rats. Ankara Univ Vet Fak Derg. 2026;73:83–92.

MLA

Deveci, Mehmet Zeki Yılmaz, et al. “Wound Healing Activities of Eucalyptol on Full Thickness Excisional Skin Wound Model in Rats”. Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol. 73, no. 1, Jan. 2026, pp. 83-92, doi:10.33988/auvfd.1677537.

Vancouver

1.Mehmet Zeki Yılmaz Deveci, Ömer Kırgız, Hüseyin Özkan, Cafer Tayer İşler, Filiz Kazak Akçakavak, Ziya Yurtal, Akın Yakan. Wound healing activities of eucalyptol on full thickness excisional skin wound model in rats. Ankara Univ Vet Fak Derg. 2026 Jan. 1;73(1):83-92. doi:10.33988/auvfd.1677537