Presence of Listeria species in ready-made meatballs offered by sale under freezing or cooling preservation

Abstract

This study was conducted to detect contamination level of Listeria species in ready-made meatballs kinds that are stored under frozen or cooled conditions. In isolations and identifications of Listeria species from the samples, method approved and suggested by USDA/FSIS (United States Department of Agriculture/Food Safety and Inspection Service) was used. The strains that
were identified to be Listeria monocytogenes with biochemical tests was verified as species through Real Time PCR method by using a primary pair specific to hly A gene location. In this study, a total number of 290 different type ready-made meatball samples were analysed. As a result of examining all samples was isolated L. monocytogenes in 32 (11.04%) samples, L. ivanovii in 9 (3.10%) samples, L. innocua in 22 (7.59%) samples, L. welchimerii in 8 (2.76%) samples and also L. seeligeri in 4 (1.38%) samples. In the serotyping of the 32 L. monocytogenes strains isolated from the samples; 15 isolated are found to be Type 1, where 3 strains are found to be Type 4, 11 strains to be type Poly and the rest 3 strains could not typified. The Mean pH and water activity values for the samples were found to be 6.62±0.56 and 0.985±0.007 respectively. In the result of the study, identifying Listeria species especially L. monocytogenes in cooled and frozen ready-made meatball samples studied suggest that such products whose consumption increased in the recent years pose important risk in terms of public health. 

Keywords

• Listeria species
• Listeria monocytogenes
• meatball
• PCR

Dondurma veya soğutma ile muhafaza edilerek satışa sunulan hazır köftelerde Listeria türlerinin varlığı

Abstract

Bu çalışma, kasap, şarküteri ve süpermarketlerde dondurulmuş veya soğutulmuş olarak muhafaza edilen ve satışa sunulan hazır köfte çeşitlerinde Listeria türlerinin kontaminasyon düzeyini ortaya koymak amacıyla yapılmıştır. Örneklerden Listeria türlerinin izolasyon ve identifikasyonunda USDA/FSIS (United States Department of Agriculture/Food Safety and Inspection Service) tarafından önerilen yöntem kullanılmıştır. Biyokimyasal testlerle L. monocytogenes olarak identifiye edilen suşların hly A gen bölgesine spesifik bir primer çifti kullanılarak Real Time PCR yöntemiyle tür olarak doğrulaması yapılmıştır. Çalışmada toplam 290 adet hazır köfte örneği analize alınmıştır. Tüm örneklerin 32 (%11.04) tanesinden L. monocytogenes, 9 (%3.10) tanesinden L. ivanovii, 22 (%7.59) tanesinden L. innocua, 8 (%2.76) tanesinden L. welchimeri ve 4 (%1.38) tanesinden de L. seeligeri izole edilmiştir. Yapılan serotiplendirmede izole edilen 32 adet L. monocytogenes türünden 15 tanesi Tip 1, 3 tanesi Tip 4 ve 11 tanesi de Tip Poli olarak tanımlanmış, 3 izolat ise tiplendirilememiştir. Tüm örneklerde ortalama pH değeri 6.62±0.56 olarak bulunurken, su aktivitesi değeri ise ortalama 0.985±0.007 olarak saptanmıştır. Çalışma sonucunda incelenen örneklerde önemli oranlarda Listeria türlerinin, özellikle de L. monocytogenes’in tespit edilmesi, son yıllarda tüketimi gittikçe artan bu ürünlerin halk sağlığı açısından önemli bir risk oluşturabileceğini göstermektedir

Keywords

• Köfte
• Listeria monocytogenes
• Listeria türleri
• PCR

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