Hypericum Perforatum Uygulanan Ehrlich Asit Solid Tümör Oluşturulmuş Farelerde Karaciğer Dokusu Adenozin Deaminaz Enzim Aktivitesi

Yıl 2019, Cilt: 2 Sayı: 4 - Ek sayı, 145 - 151, 30.12.2019

Saadet Nur Mutlu Burcu Menekşe Balkan

Öz

Kanser tedavisinde kullanılan ilaçlar hastalarda ciddi yan etkiler meydana getirmektedir. Bu sebeple kanseri tedavisinde kullanılabilecek, yan etkileri olmayan bitkisel kaynaklı ilaçların geliştirilmesi konusunda çalışmalar yapılmaktadır. Meme kanserinde karsinojenik süreç ve bazı enzimlerin aktivasyonu arasında önemli bir ilişki olduğu, kanser doku ve hücrelerinde ise ADA aktivitesinin artabileceği ya da azalabileceği bildirilmiştir. Bu çalışmada kanser tedavisinde kullanımları antioksidan etkileri bilinen H. Perforatum’un sulu ekstratının meme kanseri oluşturulan farelerde, karaciğer dokusu ADA enzim aktiviteleri üzerindeki etkilerinin araştırılması amaçlandı. Çalışmada deney hayvanları, sağlıklı kontrol grubu (Grup 1, n=8), kanserli kontrol grubu (Grup 2, n=9 ), düşük doz H. Perforatum (grup 3, n=8), yüksek doz H. Perforatum (grup 4, n=11) ve Doksorubisin (Grup 5, n=12) uygulanan gruplar olmak üzere beş gruba ayrıldı. ADA aktivitesi, Grup 2’de Grup 4’e göre arttığı (P<0.05) tespit edilmiştir.

Anahtar Kelimeler

Adenozin Deaminaz, Fare, Hypericum perforatum, Kanser

Destekleyen Kurum

Burdur Mehmet Akif Ersoy Üniversitesi BAP

Proje Numarası

0528-YL-18

Teşekkür

Bu çalışma, Burdur Mehmet Akif Ersoy Universitesi Bilimsel Araştırma Projeleri Kordinatörlüğü (0528-YL-18) tarafından desteklenmiştir.

Liver Tissue Adenosine Deaminase Enzyme Activity in Hypericum Perforatum Applied Ehrlich Acid Solid Tumor Bearing Mice

Öz

Drugs used in cancer treatment have serious side effects in patients. For this reason, studies are being carried out on the development of herbal based drugs that can be used in the treatment of cancer without side effects. It has been reported that there is an important relationship between carcinogenic process and activation of some enzymes in breast cancer and ADA activity may increase or decrease in cancer tissues and cells. The aim of this study is to investigate the effects of aqueous extract of H. perforatum on liver tissue ADA enzyme activities in breast cancerinduced mice.In the study, mice divided into five groups; healthy control group (Group 1, n = 8), cancer control group (Group 2, n = 9), low-dose H. Perforatum (group 3, n = 8), high-dose H. Perforatum (group 4), n = 11) and doxorubicin (Group 5, n = 12). Liver tissue ADA activity increased in Group 2 compared to Group 4 (P <0.05).

Anahtar Kelimeler

Adenosine deaminase, Cancer, Hypericum perforatum, Mice

Proje Numarası

0528-YL-18

Kaynakça

• Aghaeia M, Karami-Tehrania F, Salamia S, Atrib M. (2005). Adenosine Deaminase Activity in the Serum and Malignant Tumors of Breast Cancer: The Assessment of İsoenzyme ADA1 and ADA2 Activities. Clin Biochem, 38: 887-891.
• Avcı A, Kaçmaz M, Kavutcu M, Göçmen E, Durak I. (2005). Effects of on Antioxidant Extract on Adenosine Deaminase Activities in Cancerous Human Liver Tissues. International Jaurnal of Cancer Research, 1 (1-2): 53-56.
• Camici M, Tozzi MG, Allegrini S. (1990). Purine Salvage Enzyme Activities İn Normal And Neoplastic Human Tissues. Can Biochem Biophys, 2: 201-9
• Canbolat O, Durak I, Cetin R, Kavutcu M, Demirci S, Ozturk S. (1996). Activities of adenosine deaminase, 5V-nucleotidase, guanase and cytidin deaminase enzymes in cancerous and noncancerous human breast cancer. Breast Cancer Res Treat, 37: 189-93.
• Colasanti A, Kisslinger A, Liuzzi R, Quarto M, Riccio P, Roberti G, Tramantano D, Villani F. (2000). Hypericin photosensitization of tumor and metastatic cell lines of human prostate. J Photochem Photobiol, 54: 103-7. Demir H, Keskin S, Demir C, Gökyer H. (2017). Meme Kanseri Teşhisi ve Evrelerini Belirlemede Bazı Enzimlerin Tanısal Performansını ROC (Receiver Operating Characteristics) Eğrisi ile Değerlendirilmesi, http://medikalfizik.org/ uploads/files/15_mfd_kongre/17mayis_30_halitdemir.pdf Erişim Tarihi: 15.11.2017.
• Dolanmaz D (1998). Kanseröz Ve Non- Kanseröz BasBoyun Bölgesi Dokularında Adenozin Deaminaz Enzim Aktivitesinin İncelenmesi. Ankara Üniversitesi Sağlık Bilimleri Enstitüsü Ağız, Diş, Çene Hastalıkları ve Cerrahisi Anabilim Dalı Doktora Tezi, Ankara
• Durak I, Işık ACÜ, Canbolat O, Akyol Ö, Kavukçu M. (1993). Adenosine Deaminase, 5’ Nuleotidase, Xanthine Oxidase, Superoxide Dismutase And Catalase Activities İn Cancerous And Non- Cancerous Human Laryngeal Tissues. Free Radical Bio Med, 15(6): 681-684.
• Durak I, Işık AU, Canbolat O, Akyol O, Kavutçu M. (1994). Adenosine deaminase, 5V-nucleotidase, xanthine oxidase, superoxid dismutase, and catalase activities in cancerous and noncancerous human bladder tissues. Free Radical Biol Med, 16: 825- 31.
• Durak I, Çetin R, Canbolat O, Çetin D, Yurtaslanı Z, Ünal A. (1996). Adenosine Deaminase, 5’ Nucleotidase, Guanase And Cytidine Deaminase İn Gastric Tissues From Patients With Gastric Cancer. Cancer Lett, 84(2): 199- 202.
• Fox FE, Niu Z, Tobia A, Rook AH. (1998). Photoactivated hypericin is an anti- proliferative agent that induces a high rate of apoptotic death of normal, transformed, and malignant T lymphocytes: Complications for the treatment of cutaneous lympho- proliferative and inflammatory disorders. J Invest Dermatol, 111(2): 327-32
• Giusti, G. (1974) Adenosine Deaminase. In: Bergmeyer, H.U., Ed., Methods of Enzymatic Analysis, 2nd Edition, Academic Press, New York, 1092-1099.
• Guo-Shiou Liao GS, Apaya MK, Shyur LF. (2013). Review Article Herbal Medicine and Acupuncture for Breast Cancer Palliative Care and Adjuvant Therapy. Evid.-Based Complementary Altern. Med. Article ID 437948, 17.
• Ishii A. And Green H. (1973). Lethality Of Adenosine For Cultured Mammalian Cells By İnterference With Pyrimidine Biosynthesis. J. Cell Sci., 13: 419-439.
• Kamuhabwa AR, Agostinis P, D’Hallewin MA, Kasran A, De Witte PA. (2000). Photodynamic activity of hypericin in human urinary bladder carcinoma cells. Anticancer Res, 220: 2579-84
• Koizumi H, Lizuka H, Aoyagi T, Miura Y. (1983). Adenosine Deaminase İn Epidermis From Healty And Psoriatic Subjects. Arch Dermatol Res, 275: 310-4
• Liu CD, Kwan D, Saxton RE, McFadden DW. (2000). Hypericin and photodynamic therapy decreases human pancreatic cancer in vitro and vivo. J Surg Res, 93: 137-43
• Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. (1951). Protein measurement with the folin phenol reagent. J Biochem., 193: 265-275.
• Mishra R, Agarwal MK, Chansuria JPN. (2000). Serum Adenosine Deamimase Levels As An Index Of Tumor Growth İn Head And Neck Malignancy. Indian J Otolaryngol Head Neck Surg, 52(4): 360-363.
• Nishihara H, Akedo H, Okada H. (1970). Multienzyme Patterns Of Serum Adenosine Deaminase By Agar Gel Electrophoresis: An Evaluation Of The Diagnostic Value İn Lung Cancer. Clin Chim Acta, 30: 251-8
• Nogueira T, Marcelo-Curto MJ, Figueiredo AC, Barroso JG, Pedro LG, Rubiolo P, Bicchi C (2008). Chemotaxonomy of Hypericum genus from Portugal: Geographical distribution and essential oils composition of Hypericum perfoliatum, Hypericum humifusum, Hypericum linarifolium and Hypericum pulchrum. Biochem Syst Ecol. 36(1): 40-50.
• Robinson MM, Zhang X. (2011). The World Medicines Situation 2011. Traditional Medicines: Global Situation, Issues and Challenges. Geneva: World Health Organization.
• Robson NKB. (1977). Studies in the genus Hypericum L. (Guttiferae). I. Infrageneric classfication. 5: 293.
• Rogers G. (2005). Herb Consumers’ Attitudes, Preferences Profiled in New Market Study. HerbalGram American Botanical Council, 65: 60-61.
• Shore A, Dosch HM, Gelfand EW. (1981). Role of adenosine deaminase in the early stages of precursor T cell maturation. Clin Exp Immunol. 44: 152-155.
• Sufrin G, Tritsch GL, Mittelman A, Murphr GP. (1978). Adenosine Deaminase Activity İn Patients With Carcinoma of Bladder. J Urol, 119: 343-6.
• Urunsak IF, Küçükgöz-Güleç U, Paydaş S, Seydaoğlu G, Güzel A B, Vardar MA. (2012). Adenosine Deaminase Activity in Patients With Ovarian Neoplasms. Arch Gynecol Obstet, 286: 155-159.
• Walia M, Mahajan M, Singh K. (1995). Serum adenosine deaminase, 5nucleotidase and alkalin phosphates in breast cancer patients. Indian J Med Res. 101: 247–9.
• Wiid I, Seaman T, Hoal EG, Benade AJ, Van Helden PD. (2004). Total antioxidant levels are low during active TB and rise with antituberculosis therapy. IUBMB Life. 56: 101-106.