Determination of Paraoxonase Activity as a Biomarker in Dogs with Dirofilariosis

Yıl 2021, Cilt: 4 Sayı: 1, 47 - 51, 29.01.2021

Osman Kına Tünay Kontaş Aşkar Murat Güzel

Öz

Dirofilariosis is a common asymptomatic zoonotic disease in which parasite adults are primarily found in pulmonary arteries and the right-side of the heart. Interest about the role of paraoxonase (PON) activity in cardiovascular diseases is increasing day by day. In this study we aimed to define the changes in enzyme activities (AST, ALT, LDH, CK, CK-MB) with paraoxonase activity and lipid prophile (triglycerides, total cholesterol, very low-density lipoprotein, low-density lipoprotein, high-density lipoprotein) in dogs with dirofilariosis. 15 healthy dogs and 30 dogs with dirofilariosis (antigen positive for adult D. immitis and negative for microfilariae) between 4-6 years of old age were enrolled in this study.
Significantly higher serum trigliseride (p<0.01), very low-density lipoprotein (p<0.05), and low-density lipoprotein (p < 0.001) levels and aspartate aminotransferase (p<0.001), alanin aminotransferase (p<0.001), creatine kinase (p<0.001), creatine kinase MB fraction (p<0.001), lactate dehydrogenase (p<0.01) activities were found in dogs with dirofilariosis compared to healthy dogs. On the other hand, significantly lower total cholesterol (p <0.001) and high-density lipoprotein (p < 0.01) levels were found in dogs with dirofilariosis. Also, serum basal paraoxonase (p<0.01) activities of dogs with dirofilariosis were significantly lower than in the control group. In conclusion, serum bazal paraoxonase activity can be used as a marker of cardiac damage, such as LDH and CK-MB, in dogs with dirofilariosis.

Anahtar Kelimeler

Dirofilariosis, Dog, Paraoxonase, Lipid profile

Dirofilariosisli Köpeklerde Bir Biyomarker Olarak Paraoksonaz Aktivitesinin Belirlenmesi

Öz

Dirofilariosis, sivrisineklerle bulaşan özellikle kardiopulmoner hastalıklara yol açan dirofilaria türlerinin neden olduğu zoonoz bir paraziter hastalıktır. Bu çalışmada D. immitis ile enfekte köpeklerde paraoksonaz (PON) aktivitesi yanında, diğer enzim aktivite (AST, ALT, LDH, CK, CK-MB) ile lipid profili (trigliserit, total kolesterol, çok düşük dansiteli lipoprtotein, Düşük dansiteli lipoprotein, Yüksek Dansiteli lipoprotein) değişimlerinin ortaya konulması amaçlanmıştır. Çalışma, 4-6 yaş arası 30 sağlıklı ve 30 dirofilariosisli köpekde gerçekleştirilmiştir.
Dirofilariosisli köpeklerde serum aspartat aminotransferaz (p < 0.001), alanin aminotransferaz (p < 0.001), kreatin kinaz (p < 0.001) ve laktat dehidrogenaz (p < 0.01) aktivitesi ile kreatin kinaz-MB izoenzim (p < 0.001) aktivitesi, sağlıklı köpeklere göre yüksek bulunmuştur. Serum bazal paraoksonaz (p < 0.01) ise sağlıklı köpeklere göre düşük bulunmuştur. Yapılan lipid profili analizlerinde ise, dirofilariosisli köpeklerde serum trigliserid (p < 0.01), çok düşük dansiteli lipoprotein (p < 0.05) ve düşük dansiteli lipoprotein (p < 0.001) düzeyleri sağlıklı köpeklere göre yüksek bulunurken, serum total kolesterol (p < 0.001) ve yüksek dansiteli lipoprotein (p < 0.01) aktivitesi ise düşük bulunmuştur.
Araştırmadan elde edilen verilerin ışığında, serum bazal paraoksonaz aktivite düzeyi ölçümlerinin, dirofilariosisli köpeklerde meydana gelen kardiak hasarın belirlenmesinde, LDH ve CK-MB gibi yararlı bir indikatör olabileceği kanısına varılmıştır.

Anahtar Kelimeler

Dirofilariosis, Köpek, Paraoksonaz, Lipid profili

Kaynakça

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