Determination of Antimicrobial Activities of Methanolic Extracts of Some Medicinally Important Plants Found Around Çorum Province
Öz
Objective: This study aimed to investigate the antimicrobial activities of methanolic extracts obtained from ten medicinal plants traditionally used in Çorum province, Türkiye.
Materials and Methods: Plant samples were collected in the spring season, dried, and extracted using 70% methanol. The antimicrobial activities of the extracts were tested by the disk diffusion method against three Gram-positive bacteria (Bacillus subtilis, Staphylococcus aureus, Enterococcus faecalis), three Gram-negative bacteria (Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa), and two fungi (Candida albicans, Aspergillus niger). Ampicillin, Polymyxin B, and Nystatin were used as positive controls.
Results: The results showed that nine plant extracts exhibited inhibitory effects against K. pneumoniae, while all extracts inhibited B. subtilis. Seven extracts were active against E. faecalis, whereas acorn and oleaster extracts displayed limited antifungal activity against A. niger. No inhibitory activity was observed against S. aureus, E. coli, P. aeruginosa, and C. albicans.
Conclusion: The findings suggest that several plant extracts commonly used in Çorum possess notable antimicrobial potential. Extracts particularly effective against B. subtilis and E. faecalis may serve as promising sources for the development of novel natural antimicrobial agents. Further studies should focus on isolating and characterizing the bioactive compounds responsible for these effects.
Anahtar Kelimeler
Medicinal plants, methanolic extract, antimicrobial activity, Çorum
Destekleyen Kurum
TÜBİTAK 2209-A Research Project Program (Project No: 1919B012310809)
Proje Numarası
TÜBİTAK 2209-A Research Project Program (Project No: 1919B012310809)
Etik Beyan
This article does not contain any studies with human participants and/or animals performed by any of the authors. Formal consent is not required in this study.
Kaynakça
- Akbulut, S., Özkan, Z.C., & Kalankan, G. (2017). Some plants and their efects used in traditional treatment of diseases at Çorum Province in Turkey. International Journal of Secondary Metabolite, 4(3), 330–339. https://doi.org/10.21448/ijsm.373823
- Barbour, E.K., Al Sharif, M., Sagherian, V.K., Habre, A.N., Talhouk, R.S., & Talhouk, S.N. (2004). Screening of selected indigenous plants of Lebanon for antimicrobial activity. Journal of Ethnopharmacology, 93, 1–7. https://doi.org/10.1016/j.jep.2004.02.027
- Bauer, A.W., Kirby, W.M.M., Sherris, J.C., & Turck, M. (1966). Antibiotic susceptibility testing by a standardized single disk method. American Journal of Clinical Pathology, 45(4), 493–496.
- Berber, İ., Avşar, C., Çine, N., Bozkurt, N., & Elmas, E. (2013). Sinop’da yetişen bazı bitkilerin metanolik ekstraktlarının antibakteriyal ve antifungal aktivitelerinin belirlenmesi. Karaelmas Fen ve Mühendislik Dergisi, 3(1), 10–16.
- Cowan, M. M. (1999). Plant products as antimicrobial agents. Clinical Microbiology Reviews, 12(4), 564–582. https://doi.org/10.1128/CMR.12.4.564
- Cox, P.A. (1994). The ethnobotanical approach to drug discovery: Strength and limitation. In: Prance. G. T. ed. 25–40.
- Daglia, M. (2012). Polyphenols as antimicrobial agents. Current Opinion in Biotechnology, 23(2), 174–181. https://doi.org/10.1016/j.copbio.2011.08.007
- Eloff, J.N. (1998). Which extractant should be used for the screening and ısolation of antimicrobial components from plants? J. Ethnopharmacol., 60(1), 1–8. https://doi.org/10.1016/s0378-8741(97)00123-2
- Erdemir, A.D. (2001). Şifalı bitkiler: doğal ilaçlarla geleneksel tedaviler. İstanbul: Alfa Yayınları.
- Eyüboğlu, İ.Z. (1977). Anadolu halk ilaçları. İstanbul: Hürriyet Yayınları.