Morphological Characteristics and Molecular Analysis of Newly Recorded Longidorus moesicus Lamberti, Choleva & Agostinelli, 1983 (Dorylaimida: Longidoridae) From Türkiye

Yıl 2023, Cilt: 12 Sayı: 2, 187 - 198, 31.12.2023

Serkan Çelik , Faruk Akyazı

https://doi.org/10.29278/azd.1333478

Öz

Objective: This study was conducted to determine the morphological, morphometric, and molecular characteristics of Longidorus moesicus, which was first recorded in Türkiye.
Materials and Methods: A nematode survey was conducted in rose cultivation greenhouses in 2017 in Yalova province, Türkiye. Nematodes were extracted from soil samples using Cobb's sieve method and centrifugal flotation technique. Morphological characters and morphometric measurements were used to identify the obtained nematodes. Additionally, identification was confirmed by molecular analyses of the 28S rRNA gene D2/D3 expansion region sequences.
Results: Needle nematodes in the genus Longidorus (Micoletzky, 1922) include many ectoparasitic nematode species that transmit nepoviruses as well as causing damage by direct feeding on root cells. A Longidorus species was found in the examined soils.
Conclusion: To our knowledge, this is the first report of Longidorus moesicus Lamberti, Choleva & Agostinelli, 1983 in Türkiye.

Anahtar Kelimeler

Needle nematodes, Longidorus spp., Longidorus moesicus, Rosa spp.

Destekleyen Kurum

Ordu Üniversitesi Bilimsel Araştırmalar Birimi (ODUBAP)

Proje Numarası

BY-1713

Teşekkür

We thank Ordu University for their support to this project (ODUBAP; Project No, BY-1713)

Türkiye'den Yeni Bir Kayıt Longidorus moesicus Lamberti, Choleva & Agostinelli, 1983 (Dorylaimida: Longidoridae)'un Morfolojik Özellikleri ve Moleküler Analizi

Öz

Amaç: Bu çalışma, Türkiye’de ilk kayıt olan Longidorus moesicus’un morfolojik, morfometrik ve moleküler karakterlerlerinin belirlenmesi amacıyla yapılmıştır.
Matertal ve Yöntem: Yalova ili gül yetiştirilen seralarda 2017 yılında nematod sürveyi gerçekleştirilmiştir. Nematodlar, santrifüj ve Cobb elek yönteminin bir modifikasyonu ile topraktan ekstrakte edilmiştir. Elde edilen nematodların teşhisinde morfolojik karakterler ve morfometrik ölçümler kullanılmıştır. Teşhisler ayrıca 28S rRNA geni D2/D3 genleşme bölgesi dizilerinin moleküler analizleri ile doğrulanmıştır.
Araştırma Bulguları: Longidorus (Micoletzky, 1922) cinsi içerisinde yeralan iğne nematodları, bitki hücreleri ile doğrudan beslenerek zarar vermesinin yanısıra nepovirüsleri ileten çok sayıda ektoparazitik nematod türlerini içermektedir. İncelenen topraklarda Longidorus cinsi bir nematod türü tespit edilmiştir.
Sonuç: Bildiğimiz kadarıyla Longidorus moesicus Lamberti, Choleva & Agostinelli, 1983'ün Türkiye için ilk rapordur.

Anahtar Kelimeler

İğne nematodları, Longidorus spp., Longidorus moesicus, Rosa spp.

Proje Numarası

BY-1713

Kaynakça

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