Effects of Serratia grimesii Bacterial Strain on Germination of Golden Wreath Wattle (Acacia cyanophylla Lindley) Seeds under Cadmium Impact

Abstract

Golden wreath (Acacia cyanophylla Lindley) is a species belonging to the Leguminosae family, which is especially preferred in road afforestation and urban median landscape arrangements due to its resistance to air pollution. In addition to this function in road afforestation studies in landscape, Golden wreath wattle also draws attention with the visual effect it provides with its yellow flowers. Cadmium is a non-nutritive metal considered harmful to the environment and to humans. In this study, it was aimed to determine the effect of cadmium, on seed germination of A.cyanophylla. In this study, which was conducted under laboratory conditions and in petri dishes, culture media were exposed to cadmium (Cd) at concentrations of 75, 150, 225 and 300 ppm. In addition, the plant growth promoting Serratia grimesii bacterial strain was inoculated into the seeds simultaneously or alone and the germination percentage, rate and shooting number parameters were examined. Serratia grimesii bacterial strain is a bacterial strain that exhibits nitrogen fixation and ACC deaminase activity. By studying these properties of the bacteria, the germination and growth of golden wreath wattle seeds in increasing cadmium doses will create a preliminary impression about the growth and usability of this plant species in polluted environments. According to the results obtained from the study, the lowest and highest germination rates and speeds were determined at 43.33% (Bacteria) and 70% (150 ppm Cd), respectively. In parallel with this, the lowest germination rate was determined as 14.44 with bacteria and the highest rate was determined as 23.33 in 150 ppm Cd treatment. While no shoot formation was observed in the culture media where bacteria was applied together with Cd at 75, 150 and 225 ppm concentrations, the highest number of shoots was obtained in 300 ppm Cd treatment.

Keywords

• Germination
• Cadmium
• Golden Wreath Wattle
• PGPB.

Kadmiyum Etkisi Altındaki Kıbrıs Akasyası (Acacia cyanophylla Lindley) Tohumlarının Çimlenmesine Serratia grimesii Bakteri Irkının Yaptığı Etkiler

Öz

Kıbrıs akasyası (Acacia cyanophylla Lindley) kirli havaya dayanıklılık gösterdiği için özellikle yol ağaçlandırmalarında ve kent içi refüj düzenlemelerinde tercih edilen Leguminosae familyasına ait bir türdür. Kıbrıs akasyası, peyzajda yol ağaçlandırma çalışmalarındaki bu işlevinin yanı sıra sarı çiçekleri ile sağladığı görsel etki ile de dikkat çekmektedir. Bu araştırmada, kadmiyumun Acacia cyanophylla tohumlarının çimlenmesine etkisinin belirlenmesi amaçlanmıştır. Laboratuvar koşullarında ve petri kaplarında yürütülen bu çalışmada kültür ortamları 75, 150, 225 ve 300 ppm konsantrasyonlarındaki kadmiyuma (Cd) maruz bırakılmıştır. Cd bulaştırılan ortamlara ekilen ve ayrıca bu ortamlarda bitki büyümeyi teşvik edici Serratia grimesii bakteri ırkı inoküle edilen tohumların çimlenme oranı, hızı sürgün sayısı parametreleri incelenmiştir. Serratia grimesii bakteri ırkı azot bağlama ve ACC deaminaz aktivitesi gösteren bir bakteri ırkıdır. Bakterinin bu özelliklerinin çalışmasıyla Kıbrıs akasyası tohumlarının artan kadmiyum dozlarında çimlenip büyümeleri bu bitki türünün kirli ortamda yetişip kullanılabilirliği hakkında ön izlenim oluşturacaktır. Çalışmadan elde edilen sonuçlara göre çimlenme oranları ve hızları, en düşük ve en yüksek olarak sırasıyla %43,33 (Bakteri) ve %70 (150 ppm Cd) oranlarında tespit edilmiştir. Çimlenme hızı da buna paralel olarak en düşük 14,44 ile bakteri ve en yüksek 23,33 ile 150 ppm Cd uygulamasında belirlenmiştir. Bakterinin 75, 150 ve 225 ppm konsantrasyonundaki Cd ile birlikte uygulandığı kültür ortamlarında sürgün oluşumu gözlenmezken, en yüksek sürgün sayısı 300 ppm Cd uygulamasında elde edilmiştir.

Anahtar Kelimeler

• Çimlenme
• Kadmiyum
• Kıbrıs akasyası
• PGPB.

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