Prostat Adenokarsinomunun Tanısında ve Prognostik Sınıflandırmasında CCR3 Ekspresyonunun Prediktif Değeri

Yıl 2022, , 440 - 446, 04.10.2022

Ayla Solmaz Avcıkurt , Eren Altun , Fatma Bahar Sunay

https://doi.org/10.53424/balikesirsbd.1068208

Öz

Amaç: Kemokinler, enflamatuar ve immünolojik bir reaksiyonu indüklemek için hasarlı veya enfekte dokulardan salgılanan faktörlerdir. Hem kemokinler hem de reseptörleri, tümör oluşumunun kritik aktörleri olarak kabul edilir. Çalışmamız, prostat adenokarsinomunda (PCa) son zamanlarda çoğu kanser hücresi tarafından salgılandığı tespit edilen bir kemokin reseptörü olan CCR-3 ekspresyonunu araştırmayı ve Gleason prognostik derecesi ile CCR-3 seviyeleri arasındaki olası ilişkiyi ortaya koymayı amaçlamaktadır. Gereç ve Yöntem: Çalışmaya, prostatektomi yapılan PCa'lı 25 hasta ve iyi huylu prostat hiperplazisi (BPH) olan 25 hasta dahil edildi. Her iki hasta grubunda da CCR-3 ekspresyonu hem Real-Time PCR ile mRNA düzeyinde, hem de immünohistokimyasal (IHC) boyama ile protein düzeyinde saptandı. PCa ve BPH gruplarının sonuçları karşılaştırıldı. PCa grubunda CCR-3 ekspresyonu ile Gleason prognostik derecesi arasında herhangi bir ilişki de araştırıldı. Bulgular: Her ne kadar PCa hastalarında CCR-3 ekspresyonu mRNA düzeyinde anlamlı olarak yüksek bulunduysa da (p=0.001), fark protein düzeyinde anlamlı değildi (p=0.205). Yine, farklı Gleason prognostik derecelerine sahip hastaların CCR3 ekspresyon seviyeleri arasındaki fark hem mRNA hem de protein seviyelerinde anlamlı değildi. PCa hastalarında CCR-3 IHC boyaması ile toplam PSA düzeyleri (p=0.001) arasında istatistiksel olarak anlamlı pozitif korelasyon bulundu. Sonuç: CCR-3 mRNA düzeylerinin PCa tanısında faydalı olabileceği sonucuna varıldı.

Anahtar Kelimeler

CCR-3, Prostat kanseri, Gleason prognostik derecesi, Kemokin

Proje Numarası

BAP. 2017/201

Predictive Value of CCR3 Expression on Diagnosis and Prognostic Classification of the Prostate Adenocarcinoma

Öz

Objective: Chemokines are factors secreted from damaged or infected tissues to induce an inflammatory and immunological reaction. Both chemokines and their receptors are considered as critical actors of tumor formation. Our study aims to investigate CCR-3 expression, which is a chemokine receptor that has recently been found to be secreted by most cancer cells, in prostatic adenocarcinoma (PCa), and reveal any possible relation between the Gleason prognostic grade and CCR-3 levels. Material Method: The study included 25 patients with PCa who underwent prostatectomy, and 25 patients with benign prostate hyperplasia (BPH). CCR-3 was detected at mRNA level by the Real-Time PCR, and at protein level by immunohistochemical (IHC) staining in both PCa and BPH patients. Results from PCa and BPH groups were compared. In PCa group, any correlation between the CCR-3 expression and Gleason prognostic grade was also searched. Results: Although CCR-3 mRNA levels in PCa group were found to be significantly higher than the BPH group (p=0.001), the difference was not significant at the protein level (p=0.205). The difference between the CCR3 expression levels of patients with different Gleason prognostic grades was not significant at both mRNA and protein levels. A statistically significant positive correlation was found between the CCR-3 IHC staining and total PSA levels (p=0.001) in PCa patients. Conclusion: We concluded that the CCR-3 mRNA levels may be useful in the diagnosis of PCa.

Anahtar Kelimeler

CCR-3, Prostate cancer, Gleason prognostic grade, Chemokine

Destekleyen Kurum

Balıkesir University

Proje Numarası

BAP. 2017/201

Kaynakça

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