Güncel Dezenfektan Yöntemlerinin Kök Kanal Dentin Mikrosertliği Üzerine Etkisi

Yıl 2021, Cilt: 10 Sayı: 3, 405 - 409, 26.11.2021

Cihan Küden Seda Nur Karakaş

https://doi.org/10.53424/balikesirsbd.978602

Cited By: 1

Öz

ÖZ
Amaç: Bu çalışmanın amacı, fotodinamik terapi ve ozon gazının kök kanal dentin sertliğine etkisinin konvansiyonel yöntemlerle karşılaştırılarak incelenmesidir.
Gereç ve Yöntem: Çekilmiş yüz mandibular üçüncü molar dişin distal kökleri bu çalışmaya dahil edildi. Kök kanalları Reciproc (VDW, Münih, Almanya) ve VDW altın endodontik motor ile şekillendirildi ve 10 ml distile su ile irrige edildi. Son kök kanal dezenfeksiyon yöntemine göre örnekler 5 gruba ayrıldı (n=20). Grup 1 %2,5 sodyum hipoklorit + %17 EDTA (NaE) ile; grup 2 %2 klorheksidin (CHX) ile; grup 3 170 ppm ozon gazı ile (OZ); grup 4 fotodinamik tedavi (FDT) ile dezenfeksiyon işlemi tamamlandı. Grup 5 kontrol grubu olarak atanarak (DS) sadece distile su irrige edildi. Kök kanal dentin mikrosertliği Vickers test yöntemi ile değerlendirildi. Veriler, %95 güven aralığında tek yönlü varyans analizi ile değerlendirildi.
Bulgular: OZ grubu 53.83 ile en yüksek mikrosertlik değerine sahip olan grup olup NaE (48.45), CHX (48.58) ve DS (49.07) grupları ile karşılaştırıldığında anlamlı fark bulunmuştur. 53.74 mikrosertlik değeri sahip olan FDT grubu NaE ve CHX grupları ile karşılaştırıldığında anlamlı fark bulunmuştur.
Sonuç: Bu in vitro çalışmanın sınırlamaları dahilinde, dezenfeksiyon yöntemi olarak ozon veya fotodinamik tedavi, kök kanal dentin yapısının sertliğine katkıda bulunabilir.

Anahtar Kelimeler

Fotodinamik tedavi, kanal tedavisi, klorheksidin, mikrosertlik, ozon

Destekleyen Kurum

Çukurova Üniversitesi Bilimsel Araştırmalar Proje Birimi

Proje Numarası

TSA-2020-13426

Contemporary Disinfections Methods on the Microhardness of Root Canal Dentin

Öz

ABSTRACT
Aim: The aim of this study is to compare PDT and ozone with traditional disinfection methods in terms of root canal dentin microhardness.
Materials and Methods: One hundred distal roots of extracted mandibular third molars were included in this study. Root Canals were shaped with Reciproc (VDW, Munich, Germany) and VDW gold endodontic motor and were irrigated with 10ml distilled water.
The samples were divided into 5 groups according to the final root canal disinfection method (n=20). Group 1 was disinfected with 2.5% sodium hypochlorite + 17% EDTA (NaE), group 2 with 2% chlorhexidine (CHX), group 3 with 170 ppm ozone gas (OZ), group 4 with photodynamic therapy (FDT). Group 5 was assigned as the control group (DS), and only distilled water was irrigated. Root canal dentin microhardness was evaluated using the Vickers test method. Data were evaluated with one-way analysis of variance at 95% confidence interval.
Results: The OZ group was the group with the highest microhardness value of 53.83 and the differences were significant when compared with NaE (48.45), CHX (48.58) and DS (49.07) groups. There was a significant difference in the FDT group with 53.74 microhardness values when compared with the NaE and CHX groups.
Conclusion: Within the limitations of this in vitro study, ozone or photodynamic therapy as a disinfection method may contribute to the hardness of the root canal dentin structure.

Anahtar Kelimeler

Chlorhexidine, microhardness, ozone, photodynamic therapy, root canal

Proje Numarası

TSA-2020-13426

Kaynakça

• REFERENCES Abrar, E., Naseem, M., Baig, Q. A., Vohra, F., Maawadh, A. M., Almohareb, T., . . . Abduljabbar, T. (2020). Antimicrobial efficacy of silver diamine fluoride in comparison to photodynamic therapy and chlorhexidine on canal disinfection and bond strength to radicular dentin. Photodiagnosis and Photodynamic Therapy, 32, 102066.
• Akram, Z., Shafqat, S. S., Niaz, M. O., Raza, A., & Naseem, M. (2020). Clinical efficacy of photodynamic therapy and laser irradiation as an adjunct to open flap debridement in the treatment of chronic periodontitis: a systematic review and meta‐analysis. Photodermatology, photoimmunology & photomedicine, 36(1), 3-13.
• Al-Qahtani, A. S., AlZain, S. A., AlHamdan, E. M., Tulbah, H. I., Al Alsheikh, H. M., Naseem, M., & Vohra, F. (2018). A comparative evaluation of the effect of phototherapy of fiber post on its bond strength to dental composite. Photodiagnosis and Photodynamic Therapy, 24, 228-231. doi:https://doi.org/10.1016/j.pdpdt.2018.08.016
• Ari, H., Erdemir, A., & Belli, S. (2004). Evaluation of the effect of endodontic irrigation solutions on the microhardness and the roughness of root canal dentin. Journal of Endodontics, 30(11), 792-795.
• Baldasso, F. E. R., Roleto, L., Silva, V. D. d., Morgental, R. D., & Kopper, P. M. P. (2017). Effect of final irrigation protocols on microhardness reduction and erosion of root canal dentin. Brazilian Oral Research, 31.
• Bonstein, T., Mikulski, L. M., Bush, M. A., & Bush, P. J. (2010). Photoactivated disinfection of Streptococcus intermedius through dentin disc at clinically relevant intervals: An in vitro study. Archives of Oral Biology, 55(10), 771-777.
• Calt, S., & Serper, A. (2002). Time-dependent effects of EDTA on dentin structures. Journal of endodontics, 28(1), 17-19.
• Carrilho, M. R., Carvalho, R. M., Sousa, E. N., Nicolau, J., Breschi, L., Mazzoni, A., . . . Pashley, D. H. (2010). Substantivity of chlorhexidine to human dentin. Dental materials, 26(8), 779-785.
• Cecchin, D., Farina, A. P., Giacomin, M., Mattos Pimenta Vidal, C. d., Carlini-Júnior, B., & Ferraz, C. C. R. (2014). Influence of Chlorhexidine Application Time on the Bond Strength between Fiber Posts and Dentin. Journal of Endodontics, 40(12), 2045-2048. doi:10.1016/j.joen.2014.08.019
• Cruz-Filho, A. M., Sousa-Neto, M. D., Savioli, R. N., Silva, R. G., Vansan, L. P., & Pécora, J. D. (2011). Effect of chelating solutions on the microhardness of root canal lumen dentin. Journal of endodontics, 37(3), 358-362.
• de Oliveira, E. S., Souza, J. B., de Oliveira, G. J., Bernardes, C. M., Boggian, L. C., Santana, L., . . . Estrela, C. (2020). Effect of ozone and 10% sodium ascorbate on human dentin microhardness. RSBO, 17(2), 92-96.
• Deltour, M., Vincent, J., & Lartigau, G. (1970). Effet lethal de l’ozone sur certaines souches de bactéries aerobes dans un modèle de chambre pulpaire. Rev Odontostomatol Midi Fr, 15, 278-284.
• Elvis, A., & Ekta, J. (2011). Ozone therapy: A clinical review. Journal of natural science, biology, and medicine, 2(1), 66.
• Ghisi, A. C., Kopper, P. M. P., Baldasso, F. E., Stürmer, C. P., Rossi-Fedele, G., Steier, L., . . . Vier-Pelisser, F. V. (2014). Effect of super-oxidized water, sodium hypochlorite and EDTA on dentin microhardness. Brazilian dental journal, 25(5), 420-424.
• Hems, R., Gulabivala, K., Ng, Y. L., Ready, D., & Spratt, D. (2005). An in vitro evaluation of the ability of ozone to kill a strain of Enterococcus faecalis. International endodontic journal, 38(1), 22-29.
• Jeansonne, M. J., & White, R. R. (1994). A comparison of 2.0% chlorhexidine gluconate and 5.25% sodium hypochlorite as antimicrobial endodontic irrigants. Journal of Endodontics, 20(6), 276-278.
• Krithikadatta, J., Gopikrishna, V., & Datta, M. (2014). CRIS Guidelines (Checklist for Reporting In-vitro Studies): A concept note on the need for standardized guidelines for improving quality and transparency in reporting in-vitro studies in experimental dental research. Journal of Conservative Dentistry, 17(4), 301-304.
• Magni, E., Ferrari, M., Hickel, R., Huth, K. C., & Ilie, N. (2008). Effect of ozone gas application on the mechanical properties of dental adhesives bonded to dentin. Dental Materials, 24(10), 1428-1434.
• Oliveira, L. D., Carvalho, C. A. T., Nunes, W., Valera, M. C., Camargo, C. H. R., & Jorge, A. O. C. (2007). Effects of chlorhexidine and sodium hypochlorite on the microhardness of root canal dentin. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology, 104(4), e125-e128.
• Pashley, D., Okabe, A., & Parham, P. (1985). The relationship between dentin microhardness and tubule density. Dental Traumatology, 1(5), 176-179.
• Pladisai, P., Ampornaramveth, R. S., & Chivatxaranukul, P. (2016).Effectiveness of different disinfection protocols on the reduction of bacteria in Enterococcus faecalis biofilm in teeth with large root canals. Journal of Endodontics, 42(3), 460-464.
• Saghiri, M. A., Delvarani, A., Mehrvarzfar, P., Malganji, G., Lotfi, M., Dadresanfar, B., . . . Dadvand, S. (2009). A study of the relation between erosion and microhardness of root canal dentin. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology, 108(6), e29-e34.
• Saleh, A., & Ettman, W. (1999). Effect of endodontic irrigation solutions on microhardness of root canal dentine. Journal of dentistry, 27(1), 43-46.
• Sayin, T. C., Serper, A., Cehreli, Z. C., & Otlu, H. G. (2007). The effect of EDTA, EGTA, EDTAC, and tetracycline-HCl with and without subsequent NaOCl treatment on the microhardness of root canal dentin. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology, 104(3), 418-424.
• Souza, M. A., Lago, B. L. T., Pletsch, A., Binotto, A., Poletti, A., Rodrigues, F. T., . . . Palhano, H. S. (2020). Association of calcium hypochlorite, reciprocating instrumentation and photodynamic therapy: Antimicrobial analysis and effects on root dentin structure. Photodiagnosis and Photodynamic Therapy, 29, 101625.
• Souza, M. A., Trentini, B. M., Parizotto, T. F., Vanin, G. N., da Silva Piuco, L., Ricci, R., . . . Bervian, J. (2021). Influence of a glycolic acid-based final irrigant for photosensitizer removal of photodynamic therapy on the microhardness and colour change of the dentin structure. Photodiagnosis and Photodynamic Therapy, 33, 102151.
• Taneja, S., Kumari, M., & Anand, S. (2014). Effect of QMix, peracetic acid and ethylenediaminetetraacetic acid on calcium loss and microhardness of root dentine. Journal of conservative dentistry: JCD, 17(2), 155.
• Tirali, R. E., Turan, Y., Akal, N., & Karahan, Z. C. (2009). In vitro antimicrobial activity of several concentrations of NaOCl and Octenisept in elimination of endodontic pathogens. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology, 108(5), e117-e120.
• Uzunoglu, E., Aktemur, S., Uyanik, M. O., Durmaz, V., & Nagas, E. (2012). Effect of ethylenediaminetetraacetic acid on root fracture with respect to concentration at different time exposures. Journal of endodontics, 38(8), 1110-1113.