Van İlinde Ambalajsız Olarak Satılan Bazı Baharatlarda Aflatoksin ve Ağır Metal Düzeylerinin Belirlenmesi ve Ağır Metallerin Sağlık Risklerinin Değerlendirilmesi

Yıl 2022, Cilt: 11 Sayı: Supplement 1 - Veteriner Farmakoloji Kongresi Özel Sayısı, 7 - 14, 01.12.2022

Ufuk Mercan Yücel

https://doi.org/10.53424/balikesirsbd.1160866

Öz

Amaç: Bu çalışmanın amacı, Van ilinde ambalajsız olarak satılan kırmızıbiber, karabiber ve kimyon çeşitlerindeki aflatoksin ve ağır metal düzeylerinin belirlenmesi ve ağır metallerin sağlık risklerinin tahminlerinin yapılmasıdır. Gereç ve Yöntem: Aktarlardan ve marketlerden toplanan 60 adet örnekte (her baharat örneğinden 20'şer adet) aflatoksin analizi HPLC cihazı ile ağır metal analizi ise ICP-MS cihazı ile yapılmıştır. Bulgular: Baharat örneklerinin tümünde AFG1 ve AFG2, kimyon örneklerinin tümünde AFB1 ve AFB2 tespit edilmediği halde, kırmızıbiber örneklerinin tümünde, karabiber örneklerinin sadece 1 tanesinde AFB1 ve kırmızıbiber örneklerinin 18 tanesinde AFB2 belirlenmiştir. Ni, Cd, Pb ve Al’un ortalama değerleri sırasıyla karabiberde 5.08, 0.35, 2.47, 392.45 ppm, kimyonda 9.12, 0.20, 1.40, 514.4 ppm ve kırmızı biberde 8.95, 0.06, 0.11, 33.75 ppm olarak tespit edilmiştir. Arsenik ise sadece kimyonda tespit edilmiştir ve ortalama değeri 0.06 ppm'dir. Sonuç: Kırmızı biberlerin %90’ında (18 örnek) Türk Gıda Kodeksinde izin verilen sınır değerin üzerinde AFB1 saptanmıştır. Karabiberde tespit edilen Cd ve tüm baharat örneklerindeki Ni düzeyi maksimum izin verilebilir sınır değerlerin üzerindedir. Günlük alım tolerans limitleri göre baharatlardaki Ni, As ve Cd düzeyleri herhangi bir risk oluşturmadığı halde tüm baharat örneklerinde Al, karabiber örneklerinde Pb düzeyi günlük alım tolerans limitlerinin üstündedir. THQ maruziyet değerleri, 1'den küçük olduğu için baharat örnekleri kanserojen olmayan etki riski taşımamaktadır.

Anahtar Kelimeler

Baharat, Aflatoksin, Ağır Metal, Sağlık Riski Değerlendirilmesi

Destekleyen Kurum

Bu çalışma Van Yüzüncü Yıl Üniversitesi Bilimsel Araştırma Projeleri Birimi tarafından desteklenmiştir.

Proje Numarası

TYL-2017-5892

Teşekkür

Laboratuar çalışmalarına destek veren Gülümser ÇAĞLİ’ya teşekkür ederim.

Determination of Aflatoxin and Heavy Metal Levels in Some Spices Sold as Unpackaged in Van Province and Health Risks Assessment of Heavy Metals

Öz

Aim: This study aimed to determine the aflatoxin and heavy metal levels in the samples of unpackaged red pepper, black pepper and cumin sold in Van and to estimate the health risks of heavy metals. Materials and Methods: In 60 samples (20 of each spice sample) obtained from herbal shops and markets, aflatoxin analysis was done with HPLC device and heavy metal analysis was done with ICP-MS device. Results: Although AFG1 and AFG2 were not detected in all spice samples, AFB1 and AFB2 were not detected in all cumin samples, AFB1 was detected in all red pepper samples and only one black pepper samples, and AFB2 in 18 red pepper samples. As a result of heavy metal analysis, the average concentration of Ni, Cd, Pb and Al were 5.08, 0.35, 2.47, 392.45 ppm in black pepper, 9.12, 0.20, 1.40, 514.4 ppm in cumin and 8.95, 0.06, 0.11, 33.75 ppm in red pepper, respectively. Arsenic was detected only in cumin and its average concentration was 0.06 ppm. Conclusion: In 90% (18 samples) of red pepper samples, AFB1 was detected above the permisisble limit value in Turkish food codex. The Cd level in black pepper samples and Ni levels in all spice samples determined in this study were above the limit values. According to daily intake tolerance limits, although Ni, As, and Cd levels in spices do not pose any risk, Al in all spice samples and Pb levels in black pepper samples are above the daily intake tolerance limits. Spice samples do not pose risk of non-carcinogenic effects, as THQ exposure values are less than 1.

Anahtar Kelimeler

Spice, Aflatoxin, Heavy Metal, Health Risk Assessment

Proje Numarası

TYL-2017-5892

Kaynakça

• Agaoglu, S. (1999). Investigation of the incidence of aflatoxin B1 existence in crushed red peppers sold unpacked in Van. Van Medical Journal, 6(4), 28-30.
• Allen, S. E. (1986). Chemical analysis. In: Moore PD, Chapman SB, editors. Methods in plant ecology. Oxford, London: Blackwell Scientific Publication, Pp. 285-344.
• Anonymous (2011). Turkish Food Codex Regulation on Contaminants, Upper Limits Allowed for Aflatoxins and Ochratoxin A in Foods. https://www.resmigazete.gov.tr/eskiler/2011/12/20111229M3-8.htm.
• Anonymous, (2022). IPCS INCHEM. 658. Arsenic. (WHO Food Additives Series 24). http://www.inchem.org/documents/jecfa/jecmono/v024je08.htm. Access Date: 28.07.2022.
• AOAC (2000). Aflatoxin Extraction Method; Application Note for Analysis in Hazelnuts Using Aflaprep. Rhone-Diognostics Thecnologies Instructions; Arlington, VA, 999.07.
• Asomugha, R. N., Udowelle, N. A., Offor, S. J., Njoku, C. J., Ofoma, I. V., Chukwuogor, C. C., & Orisakwe, O. E. (2016). Heavy metals hazards from Nigerian spices. Roczniki Państwowego Zakładu Higieny, 67(3), 309-314.
• Aydin, A., Erkan, M. E., Baskaya, R., & Ciftcioglu, G. (2007). Determination of aflatoxin B1 levels in powdered red pepper. Food Control, 18, 1015-1018.
• Bircan, C. (2005). The determination of aflatoxins in spices by immunoaffinity column extraction using HPLC. International Journal of Food Science and Technology, 40, 929-934.
• Castro-González, M. I. & Méndez-Armenta, M. (2008). Heavy metals: Implications associated to fish consumption. Environmental Toxicology and Pharmacology, 26(3), 263-271. DOI: 10.1016/j.etap.2008.06.001.
• Demircioglu, S., & Filazi, A. (2010). Investigation of Aflatoxin Residues in Red Peppers Produced in Turkey. Journal of the Veterinary Medical Association, 81(2), 63-66.
• Dogan, M., Saçmaci, Ş., Kalkan, G., Kartal, Ş. (2014). Determination of Some Heavy Metal Concentration Levels in Red Chilli Powder Samples, III. Artwork Analysis Workshop, 15-18 May, Gaziosmanpasa University, Tokat.
• El Mahgubi, A., Puel, O., Bailly, S., Tadrist, S., Querin, A., Ouadia, A., Oswald, I. P., & Bailly, J. D. (2013). Distribution and toxigenicity of Aspergillus section Flavi in spices marketed in Morocco. Food Control, 32, 143-148.
• EPA, U. S. (2016). Integrated Risk Information System. Erişim adresi: https://www.epa.gov/iris/ EPA, U.S. (2019). Regional screening levels (RSLs) equations. https://www.epa.gov/risk/regional-screeninglevels-rsls-equations.
• Freedman, B. (1995). Environmental Ecology, The Ecological Effects of Pollution, Disturbance and Other Stresses, Academic Press.
• Gecan, J. S., Bandler, R., Glaze, L. E., & Atkinson, J. C. (1986). Microanalytical quality of ground and unground marjoram, sage and thyme, ground allspice, black pepper and paprika. Journal of Food Protection, 49(3), 216-221.
• Golge, O., Hepsag, F., & Kabak, B. (2013). Incidence and level of aflatoxin contamination in chilli commercialised in Turkey. Food Control, 33, 514-520.
• Hazir, Z., & Coksoyler N, (1998). Aflatoxin levels in red peppers obtained in different regions and by different methods, Food Engineering Congress and Exhibition, 479-483. 16-18 September, Gaziantep-Turkey.
• Inam, F., Deo, S., & Narkhede, N. (2013). Analysis of minerals and heavy metals in some spices collected from local market. IOSR Journal of Pharmacy and Biological Sciences, 8 (2), 40-43.
• Iqbal, S. Z., Asi, M. R., Zuber, M., Akhtar, J., & Saif, M.J. (2013). Natural occurrence of aflatoxins and ochratoxin A in commercial chilli and chilli sauce samples. Food Control, 30, 621-625.
• Jarup, L. (2003). Hazards of heavy metal contamination, British medical bulletin, 68(1), 167-82.
• JECFA (2011). Summary and conclusions of the seventy-fourth meeting, Rome, 14-23 June 2011, JECFA/74/SC.
• Kahvecioğlu O, Kartal G, Guven A, Timur S. Environmental effects of metals-I. ITU Metallurgical and Materials Engineering Department Lecture Notes, 2008.
• Kanbur, M., Liman, B. C., Eraslan, G., Altinordulu, S. (2006). Quantitative analysis of red peppers for consumption in Kayseri by enzyme Immunoassay (EIA). Journal of Erciyes University Faculty of Veterinary Medicine, 3(1), 21-24.
• Mohammadi, A., Zarei, A., Majidi, S., Ghaderpouryd, A., Hashempour, Y., Saghi, M., Alinejad, A., Yousefih, M., Hosseingholizadehi, N., & Ghaderpoori, M. (2019). Carcinogenic and non-carcinogenic health risk assessment of heavy metals in drinking water of Khorramabad, Iran. MethodsX, 6,1642–1651.
• Nkansah, M. A., & Amoako, C. O. (2010). Heavy metal content of some common spices available in markets in the Kumasi metropolis of Ghana, American Journal of Scientific and Industrial Research, 1(2), 158-63.
• Okcu, M., Tozlu, E., Kumlay, A. M. & Pehluvan, M (2009). Effects of heavy metals on plants. Alınteri, 17 (B), 14-26. Ozbey, F., & Kabak, B. (2012). Natural co-occurrence of aflatoxins and ochratoxin A in spices. Food Control, 28, 354-361.
• Ozbey, F., & Kabak, B. (2012). Natural co-occurrence of aflatoxins and ochratoxin A in spices. Food Control, 28, 354-361
• Salari R, Habibi Najafi MB, Boroushaki MT, Mortazavi SA, &Najafi MF (2012). Assessment of the microbiological quality and mycotoxin contamination of Iranian red pepper spice. Journal of Agricultural Science and Technology, 14, 1511-1521.
• Soliman Naglaa, F. E. (2015). Metals contents in spices and herbs available on the Egyptian market: assessment of potential human health risk. The Open Conference Proceedings Journal, 6, 24-29.
• Traina, A., Bono, G., Bonsignore, M., Falco, F., Giuga, M., Quinci, E. M., ... & Sprovieri, M. (2019). Heavy metals concentrations in some commercially key species from Sicilian coasts (Mediterranean Sea): Potential human health risk estimation. Ecotoxicology and Environmental Safety, 168, 466-478.
• Unal, B., Yilmaz, U. G., Gokoglu, M. & Ozvarol, Y. (2019). Determination of the Biological Characteristics and Heavy Metal Levels (Pb, Cd, Hg, Cu, Zn, Co, Ni) of the Fossil Mussel (Lithophaga lithophaga L. 1758) (Meat, Shell, Rock) Showing the Distribution in the Antalya Gulf. Bilim Armonisi , 2 (1) , 33-47 .
• Uner, Y., Aksu, H., & Ergun, Ö. (2000). Effects of spice on various microorganisms, Journal of Istanbul University Faculty of Veterinary Medicine, 26 (1), 1-10.
• USEPA (U.S. Environmental Protection Agency). (2019). Regional screening levels (RSLs) – equations. https://www.epa.gov/risk/regional-screening-levels-rsls-equations.
• USEPA (US Environmental Protection Agency). (2000). Guidance for Assessing Chemical Contaminant Data for Use in Fish Advisories, Volume II. Risk Assessment and Fish Consumption Limits. EPA 823-B-00-008. United States Environmental Protection Agency, Washington, DC.
• Williams, J.H., Phillips, T. D., Jolly, P. E., Stiles, J. K.,Jolly, C. M., & Aggerwal, D. (2004). Human aflatoxicosis in developing countries: a review of toxicology, exposure, potential health consequences, and interventions, The American Journal of Clinical Nutrition, 80, 1106-1122.
• WHO (2007). WHO guidelines for assessing quality of herbal medicines with reference to contaminants and residues. Geneva.
• WHO/FAO (2011). Food Standards Programme Codex Committee on Contaminants in Foods. Fifth Session Codex Alimentarius Commission, The Hague, The Netherlands.
• Yogendrarajah, P., Jacxsens, L., De Saeger, S., & De Meulenaer, B. (2014). Cooccurrence of multiple mycotoxins in dry chilli (Capsicum annum L.) samples from the markets of Sri Lanka and Belgium. Food Control, 46, 26-34.