Cinnamomum verum Uçucu Yağının Pseudomonas aeruginosa ve Acinetobacter baumannii Üzerindeki Anti-biyofilm ve Anti-quarum Sensing Aktivitesi

Yıl 2023, Cilt: 12 Sayı: 1, 169 - 176, 15.03.2023

Esin Kıray

https://doi.org/10.53424/balikesirsbd.1071396

Öz

ÖZ
Amaç: Günümüzde mevcut kullanımdaki antimikrobiyaller üzerinde dirençli bakterilerinin sayısının artması halk sağlığı açısından büyük tehlike oluşturmaktadır. Çoklu ilaca dirençli patojenlerin ortaya çıkması, alternatif tedavi stratejilerinin araştırılmasını zorunlu hale getirmiştir. Bitki uçucu yağları hastalıkların tedavisinde toksik olmayan, uygun maliyetli ve erişilebilir olması nedeni ile alternatif antimikrobiyaller olarak değerlendirilmektedir. Bu çalışmada, Cinnamomun verum yaprağı esansiyel yağının (EY) Pseudomonas aeruginosa ve Acinetobacter baumannii üzerindeki antibakteriyel ve anti-biyofilm etkisi ile anti-quarum sensing (QS) özelliklerini değerlendirmek amaçlamıştır. Gereç ve Yöntem: Çalışmada C. verum EY’nın P. aeruginosa ve A. baumannii üzerindeki Minimum İnhibitör Konsantrasyon (MIC) ve Minimum Bakterisidal Konsantrasyon (MBC) ları belirlenmiştir. Minimum inhibitör konsantrasyonlarının (MIC'ler) belirlenmesinde mikrodilüsyon yöntemi kullanılmıştır. C. verum EY’nin kimyasal içeriği, Gaz Kromatografisi-Kütle Spektrometrisi (GC-MS) kullanılarak belirlenmiştir. Bulgular: C. verum EY test edilen suşlara karşı 0,5 ve 1,0 mg/mL oranlarında MIC ve MBC göstermiştir. C. verum EY’nin 2 mg/mL konsantrasyonda yüksek oranda anti-QS aktivite gösterdiği belirlenmiştir. GC-MS sonucuna göre C. verum yağında en bol bulunan bileşik sinnamaldehit olarak bulunmuştur. Sonuç: C. verum EY’nin bakteriyel biyofilmlerin neden olduğu enfeksiyonlara karşı potansiyel kullanım alanı oluşturabilecek etkinliğe sahiptir. C. verum EY’lerin anti-QS aktivitesi üzerine yapılan çalışmalar sınırlı olup, bu alanda daha çok patojen mikroorganizma üzerindeki anti-QS aktivitesi araştırılmalıdır.

Anahtar Kelimeler

Cinnamomun verum, Uçucu yağ, Anti-biyofilm

Destekleyen Kurum

Kırşehir Ahi Evran Üniversitesi Bilimsel Araştırma Projeleri

Proje Numarası

Proje No: PYO-FEN.4001.16.012

Teşekkür

Bu araştırma Kırşehir Ahi Evran Üniversitesi Bilimsel Araştırma Projeleri Proje No: PYO-FEN.4001.16.012 tarafından desteklenmiştir.

Anti-biofilm and Anti-quarum Sensing Activity of Cinnamomum verum Essential Oil on Pseudomonas aeruginosa and Acinetobacter baumannii

Öz

Objective: The increase in the number of bacteria resistant to antimicrobials in current use poses a great danger to public health. The emergence of multi-drug-resistant pathogens on antimicrobials in current use has made it necessary to search for alternative treatment strategies. Plant essential oils are considered as alternative antimicrobials in the treatment of diseases because they are non-toxic, cost-effective and accessible. This study aimed to evaluate the antibacterial and antibiofilm effect and anti-quarum sensing (QS) properties of Cinnamomun verum leaf essential oil on Pseudomonas aeruginosa and Acinetobacter baumannii. Materials and Methods: In the study, Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of EO on P. aeruginosa and A. baumannii were determined. The microdilution method was used to determine the minimum inhibitory concentrations (MICs). The chemical content of C. verum EO was determined using Gas Chromatography-Mass Spectrometry (GC-MS). Results: C. verum EO showed MIC and MBC at 0.5 and 1.0 mg/mL versus strains tested. It was determined that C. verum EO showed high anti-QS activity at a concentration of 2 mg/mL. According to GC-MS results, cinnamaldehyde was found to be the most abundant compound in C. verum EO. Conclusion: C. verum EO has potential to be used against infections caused by bacterial biofilms. Studies on the anti-QS activity of C. verum EOs are limited, and the anti-QS activity on pathogenic microorganisms should be investigated in this area.

Anahtar Kelimeler

Cinnamon verum, Essential oil, Anti-biofilm, Anti-quarum detection

Proje Numarası

Proje No: PYO-FEN.4001.16.012

Kaynakça

• Abudoleh, S. M., and Mahasneh, A. M. (2017). Anti-Quorum Sensing Activity of Substances Isolated from Wild Berry Associated Bacteria, Avicenna J Med Biotechnol, 9(1), 23-30.
• Alibi, S. (2020). Anti-oxidant, antibacterial, anti-biofilm, and anti-quorum sensing activities of four essential oils against multidrug-resistant bacterial clinical isolates. Comparative Study Curr Res Transl Med. 68 (2), 59-66. doi: 10.1016/j.retram.2020.01.001.
• Alva, P. P. (2021). Isolation and identification of quorum sensing antagonist from Cinnamomum verum leaves against Pseudomonas aeruginosa. Life Sciences, 267(15), 118878.
• Barzegari, A. (2020).The Battle of Probiotics and Their Derivatives Against Biofilms. Infect Drug Resist, 13, 659-672. https://doi.10.2147/IDR.S232982.
• Bassettı, M. (2018). How to manage Pseudomonas aeruginosa infections. Drugs Context 7, 1-18.
• Bhagat, K. (2019). Design, Synthesis, Antimicrobial Evaluation, and Molecular Modeling Studies of Novel Indolinedione Coumarin Molecular Hybrids. ACS Omega, 4(5), 8720–8730. doi: 10.1021/acsomega.8b02481.
• Brackman, G. (2008). Cinnamaldehyde and cinnamaldehyde derivatives reduce virulence in Vibrio spp. by decreasing the DNA-binding activity of the quorum sensing response regulator LuxR. BMC Mikrobiyol, 8, 149 doi: 10.1186/1471-2180-8-149.
• Chao, S. (2000). Screening for Inhibitory Activity of Essential Oils on Selected Bacteria, Fungi and Viruses. J. Essential Oil Res, 12 (5), 639–649. 10.1080/10412905.2000.9712177 .
• Ciofu, O., and Tolker-Nielsen, T. (2019). Tolerance and resistance of Pseudomonas aeruginosa biofilms to antimicrobial agents- How P. aeruginosa can escape antibiotics. Front Microbiol. 10, 913. doi: 10.3389/fmicb.2019.00913.
• Coenye, T., and Nelis, H. J. (2010). In vitro and in vivo model systems to study microbial biofilm formation. J. Microbiol. Methods. 83:89–105. doi: 10.1016/j.mimet.2010.08.018.
• Firmino, D. F. (2018). Antibacterial and Antibiofilm Activities of Cinnamomum Sp. Essential Oil and Cinnamaldehyde: Antimicrobial Activities. Scientific World Journal. 7405736. doi: 10.1155/2018/7405736.
• Gerdt, J. P., and Blackwell, H. E. (2014). Competition studies confirm two major barriers that can preclude the spread of resistance to quorum-sensing inhibitors in bacteria. ACS Chem Biol, 9, 2291-2299. doi: 10.1021/cb5004288.
• Haddı, K. (2017). Cinnamon Oil, in: Leo ML and Nollet HSR (Ed), Green Pesticides Handbook, Essential Oils for Pest Control. 1st Edition. Boca Raton: CRC Press 523, 117-150.
• Jayaprakasha, G. K., and Rao, L. J. M. (2011). Chemistry, biogenesis, and biological activities of Cinnamomum zeylanicum. Crit Rev Food Sci Nutr, 51, 547-562.
• Kalia, M. (2015). Effect of Cinnamon Oil on Quorum Sensing-Controlled Virulence Factors and Biofilm Formation in Pseudomonas aeruginosa. PLoS One, 10 (8), e0135495. doi: 10.1371/journal.pone.0135495.
• Kim, Y. G. (2015). Cinnamon bark oil and its components inhibit biofilm formation and toxin production. Int J Food Microbiol. 16, 195, 30-39. doi: 10.1016/j.ijfoodmicro.2014.11.028.
• Kiray, E. Antibiofilm and Anti-Quorum Sensing Activities of Vaginal Origin Probiotics. Eur J Biol 2021; 80(2): 82-90. doi.10.26650/EurJBiol.2021.932640.
• Mayaud, L. (2008). Comparison of bacteriostatic and bactericidal activity of 13 essential oils against strains with varying sensitivity to antibiotics. Comparative Study Lett Appl Microbiol, 47 (3), 167-73.
• Rad, J. S. (2021). Cinnamomum Species: Bridging Phytochemistry Knowledge, Pharmacological Properties and Toxicological Safety for Health Benefits. Front Pharmacol, 11(12), 600139. doi: 10.3389/fphar.2021.600139.
• Rao, P. V., and Gan, S. H. (2014). Cinnamon: a multifaceted medicinal plant. Evid Based Complement Alternat Med, 642942. Schmıdt, E. (2006). Composition and antioxidant activities of the essential oil of Cinnamon (Cinnamomum zeylanicum Blume) leaves from Sri Lanka. J Essent Oil Bear Pl 9, 170-182. doi.org/10.1080/0972060X.2006.10643490.
• Sharma, D. (2019). Antibiotics versus biofilm: an emerging battleground in microbial communities. Antimicrob Resist Infect Control, 8, 76.
• Sienkiewicz, M. (2014). The Biological Activities of Cinnamon, Geranium and Lavender Essential Oils. Molecules. 19(12), 20929–20940. doi: 10.3390/molecules191220929.
• Theodora, N. A. (2019). Screening and quantification of anti-quorum sensing and antibiofilm activities of phyllosphere bacteria against biofilm forming bacteria. BMC Res Notes, 12(1), 732. doi.10.1186/s13104-019-4775-1.
• Uğur, M., and Genç, S. (2019). Three Year Resistance Profile of Acinetobacter baumannii and Pseudomonas aeruginosa Strains Isolated from Intensive Care Units. Turk J Intensive Care, 17, 130-7. https://doi10.4274/tybd.galenos .2018.94103.
• Wijesinghe, G. (2021). In-vitro Antibacterial and Antibiofilm Activity of Cinnamomum verum Leaf Oil against Pseudomonas aeruginosa, Staphylococcus aureus and Klebsiella pneumoniae. An Acad Bras Cienc, 93(1), e20201507. doi: 10.1590/0001-3765202120201507.
• Yap, P. S. (2014). Some evidences on the mode of action of Cinnamomum verum bark essential oil, alone and in combination with piperacillin against a multidrug resistant Escherichia coli strain. J Microbiol Biotechnol, 25 (8), 1299-306. doi: 10.4014/jmb.1407.07054.
• Zhao, X. (2020). Quorum-Sensing Regulation of Antimicrobial Resistance in Bacteria. Microorganisms, 8(3), 425. https://doi.10.3390/microorganisms8030425.
• Zhong, C. (2019). Design and synthesis of new N-terminal fatty acid modified-antimicrobial peptide analogues with potent in vitro biological activity. European Journal of Medicinal Chemistry, 182, 111636. https://doi.org/10.1016/j.ejmech.2019.111636.