Beyaz Çürüklük Mantarı ile Muamele Edilen Buğday Sapının FTIR Analizi

Öz

Mantarlar küresel karbon, mineral ve azot döngüsünde önemli bir role sahiptir. Mantarların bitkisel olarak büyümesi lignoselülozik atıkların değerlendirilmesinde önemli bir rol almasından dolayı hem akademik hem de ticari çevrelerin ilgisini çekmiştir. Bu çalışmada, beyaz çürüklük mantarı Pleurotus ostreatus ile üç farklı sürede inkübasyona maruz bırakılann buğday sapının kimyasal bileşimindeki değişimler Fourier dönüşümlü kızılötesi spektroskopisi ile tespit edilmiştir. Buğday sapı Pleurotus ostreatus mantarı ile 10, 20 ve 30 gün süreyle inkübe edilmiş ve her bir inkübasyon süresinin sonunda Fourier dönüşümlü kızılötesi spektrumları belirlenmiştir. Buğday sapının ana bileşenlerini oluşturan polisakkaritler, lipitler, proteinler ve kitin Fourier dönüşümlü kızılötesi spektroskopisindeki dalga boylarının nispi artış ve azalışa göre kimyasal bileşimindeki değişimler tespit edilmiştir. Pleurotus ostreatus (PO) mantarı ile muameleye uğratılmış buğday sapının Fourier dönüşümlü kızılötesi (FTIR) spektrumların da 3000-2825 cm-1 pik aralığında deformasyon, 1770 - 1520 cm-1, 1425 - 1390 cm-1, 1375 - 1300 cm-1, 896 cm-1 ve 800 cm-1 piklerinin ise ortaya çıktığı tespit edilmiştir.

Anahtar Kelimeler

• Anahtar Kelimeler − Beyaz çürüklük mantarı
• FTIR
• Buğday sapı
• Biyolojik bozunma
• Pleurotus ostreatus

Wheat Straw Treated with White-Rot Fungus FTIR Analysis

Abstract

Fungi are an important part of the natural world, and they play a significant part in the recycling of minerals and carbon, as well as in the nitrogen-fixing cycle. The vegetative growth of fungi has attracted the attention of both cademic and commercial circles due to its important role in the utilization of lignocellulosic wastes. Fourier transform infrared spectroscopy was used to detect changes in the chemical composition of wheat straw incubated with the white rot fungus Pleurotus ostreatus for three different periods. At the conclusion of each incubation period, FTIR spectra were calculated from wheat straw that had been incubated with Pleurotus ostreatus fungus for 10, 20, and 30 days. Changes in the chemical composition of polysaccharides, lipids, proteins, and chitin, which are the main components of wheat straw, were detected using fourier transform infrared spectra spectroscopy and the relative increase and decrease in wavelengths. In the Fourier transform infrared spectra (FTIR) of wheat straw treated with Pleurotus ostreatus (PO) mushroom, deformation in the peak range of 3000-2825 cm-1, 1770 - 1520 cm-1, 1425 - 1390 cm-1, 1375 - 1300 cm-1, 896 cm-1 and 800 cm-1 peaks appeared

Keywords

• White rot fungi
• FTIR
• Wheat Straw
• Biodegradation
• Pleurotus ostreatus

Kaynakça

1. Appels, F. V., Camere, S., Montalti, M., Karana, E., Jansen, K. M., Dijksterhuis, J., and Wösten, H. A. (2019). Fabrication factors influencing mechanical, moisture-and water-related properties of mycelium-based composites. Materials & Design, 161, 64-71.
2. Can. A, ve Sivrikaya, H. (2017). Mantar tahribatına uğramış titrek kavak odununun FT-IR yöntemiy-le kimyasal analizi. Bartın Orman Fakültesi Dergisi, 19(1), 139-147.
3. Can, A., Tomak, E. D., Ermeydan, M. A., & Aykanat, O. (2023). Synergic effect of basalt/wood fiber reinforced polylactic acid hybrid biocomposites against fungal decay. European Polymer Journal, 112246.
4. Eriksson KEL, Blanchette R, Ander P (2012). Microbial and enzymatic degradation of wood and wood components. Springer Science and Business Media.
5. Haneef, M., Ceseracciu, L., Canale, C., Bayer, I. S., Heredia-Guerrero, J. A., and Athanassiou, A. (2017). Advanced materials from fungal mycelium: fabrication and tuning of physical properties. Scientific reports, 7(1), 1-11.
6. Hatakka, A. (1994). Lignin-modifying enzymes from selected white-rot fungi: production and role from in lignin degradation. FEMS microbiology reviews,13(2-3), 125-135.
7. Klemm D., Phillip B., Heinze T., Heinze U. ve Wagenknetch W. (1998). Comprehensive cellulose chemistry, Wiley, Weinheim, 1, 1-7.
8. Martínez, Á. T., Speranza, M., Ruiz-Dueñas, F. J., Ferreira, P., Camarero, S., Guillén, F., ... & Río Andrade, J. C. D. (2005). Biodegradation of lignocellulosics: microbial, chemical, and enzymatic aspects of the fungal attack of lignin. INTERNATIONAL MICROBIOLOGY 8:195-204

9. Mohebby, B. (2005). Attenuated total reflection infrared spectroscopy of white-rot decayed beech wood, International Biodeterioration & Biodegradation, 55, 4, 247-251.
10. Pandey, K. K.ve Pitman, A. J. (2003). FTIR studies of the changes in wood chemistry following decay by brown-rot and white-rot fungi, International Biodeterioration & Biodegradation, 52, 3, 151-160.
11. Petrou M, Edwards H. G, Janaway R. C, Thompson G. B, Wilson A. S (2009). Fourier-transform Raman spectroscopic study of a Neolithic waterlogged wood assemblage. Analytical and bioanalytical chemistry, 395(7), 2131-2138.
12. Pena, R., Lang, C., Naumann, A., Polle, A., (2014). Ectomycorrhizal identification in environmental samples of tree roots by Fourier-transform infrared (FTIR) spectroscopy. Front. Plant Sci. 5.
13. Schwanninger, M. J. C. R., Rodrigues, J. C., Pereira, H. ve Hinterstoisser, B. (2004). Effects of short-time vibratory ball milling on the shape of FT-IR spectra of wood and cellulose, Vibrational Spectroscopy, 36, 1, 23-40.
14. Sonnenberg, A. S., and Hendrickx, P. M. (2006). Evaluation of Pleurotus eryngii strains (No. 2006-14). PPO Paddestoelen.
15. Tomak, E. D., Can, A., & Ermeydan, M. A. (2023). Biodegradability of Poly (Ɛ-Caprolactone) Modified Wood by Decaying Fungi. Journal of Polymers and the Environment, 1-15.
16. Wösten, H. A. (2019). Filamentous fungi for the production of enzymes, chemicals and materials, Current Opinion in Biotechnology, 59, 65-70.