Investigation of the antimicrobial activity of herbal cream prepared with aqueous extracts of walnut (Juglans regia L.) leaves and shells

Yıl 2025, Cilt: 6 Sayı: 3, 149 - 154, 30.12.2025

Müjgan Arslan , Adem Önal , Bilge Hilal Çadırcı , Sedanur Gökçe , Mustafa Şimşek

https://doi.org/10.53445/batd.1809617

Öz

This study investigated the antimicrobial properties of creams we developed using aqueous extracts from the bark and leaves of walnut (Juglans regia L.), a plant used for therapeutic purposes in traditional folk medicine, at various concentrations and formulations. The antimicrobial activity of the extracts was investigated against Candida species (Candida albicans, Candida utilis) and Gram-positive bacteria (Staphylococcus aureus, Streptococcus pyogenes) using the disk diffusion method. It has been observed that the cream formulation developed with leaf and leaf-bark mixture has significant activity on Candida albicans. In addition, creams derived from the aqueous extract were observed to be active against all microorganisms at various concentrations. As a results, the laboratory demonstration of the antimicrobial activity of a semi-solid cream formulation prepared with walnut shell and leaf extracts is promising for the development of plant-based, low-side-effect, and economical treatment alternatives.

Anahtar Kelimeler

Walnut , traditional medicine , medicinal plant , topical cream , antimicrobial activity

Ceviz (Juglans regia L.) yaprak ve kabuklarının sulu ekstraktlarıyla hazırlanan bitkisel kremin antimikrobiyal aktivitesinin araştırılması

Öz

Bu çalışmada, geleneksel halk hekimliğinde tedavi amaçlı kullanılan bir bitki olan ceviz (Juglans regia L.) kabuğu ve yapraklarından elde edilen sulu ekstraktlar kullanılarak geliştirdiğimiz kremlerin antimikrobiyal özellikleri, çeşitli konsantrasyon ve formülasyonlarda incelenmiştir. Ekstraktların antimikrobiyal aktivitesi, disk difüzyon yöntemi kullanılarak Candida türlerine (Candida albicans, Candida utilis) ve Gram pozitif bakterilere (Staphylococcus aereus, Streptococcus pyogenes) karşı araştırılmıştır. Yaprak ve yaprak-kabuk karışımıyla geliştirilen krem formülasyonunun Candida albicans üzerinde önemli bir aktiviteye sahip olduğu gözlemlenmiştir. Ayrıca, sulu ekstrakttan elde edilen kremlerin, çeşitli konsantrasyonlarda tüm mikroorganizmalara karşı etkili olduğu gözlemlenmiştir. Sonuç olarak, ceviz kabuğu ve yaprak ekstraktlarıyla hazırlanan yarı katı bir krem formülasyonunun antimikrobiyal aktivitesinin laboratuvar ortamında gösterilmesi, bitki bazlı, düşük yan etkili ve ekonomik tedavi alternatiflerinin geliştirilmesi açısından umut vericidir.

Anahtar Kelimeler

Ceviz , geleneksel tıp , tıbbi bitki , topikal krem , antimikrobiyal aktivite

Kaynakça

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