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At Kuyruğu (Equisetum arvense) Bitkisinin Su ve Etanol Ekstrelerinin Fenolik Bileşik İçeriğinin Belirlenmesi

Yıl 2021, Cilt 2, Sayı 2, 3 - 9, 19.05.2021

Öz

Equisetum arvense, halk arasında bilinen adıyla atkuyruğu, geleneksel tıpta genellikle, yara, romatizma, tüberküloz, deri hastalıkları, saç dökülmesi, prostat, astım ve üriner sistem hastalıklarında tedavi edici ve ağrı kesici olarak kullanılır. Equisetum arvense yapısında birçok fenolik bileşen içerir bu bileşenler yüksek antioksidan aktivite gösterir.
Bu çalışmanın amacı aktardan satın alınan atkuyruğu bitkisinin etanol ve su ekstrelerinin Diyot Array Detektörlü Yüksek Performans Sıvı Kromatografisi (HPLC-DAD) ile bireysel fenolik bileşik kompozisyonunun incelenmesidir.
Çalışmamızın sonucunda atkuyruğunun halk arasında kullanılan şekli olan su ekstresinde fenolik bileşik miktarları kilogram kuru bitki başına; 4-hidroksibenzoik asit (1.535 µg/kg), rutin (14.383 µg/kg), salisilik asit (9.639 µg/kg,) vanilik asit (2.32 µg/kg) ve chicoric asit (21.313 µg/kg) olarak bulunurken etanol ekstresinde rutin (6.23695 µg/kg), quercetin (32.9995 µg/kg), gallik asit (0.95 µg/kg), epikatesin (24.97 µg/kg), p-kumarik asit (5.97495 µg/kg), chicoric asit (39.98495 µg/kg) ve sinamik asit (1.7695 µg/kg) olarak bulunmuştur.

Kaynakça

  • Referans1 Al-Snafi. P. D. A. E. (2017). The pharmacology of Equisetum arvense- A review. IOSR Journal of Pharmacy (IOSRPHR). 07(02). 31–42. https://doi.org/10.9790/3013-0702013142
  • Referans2 Badole. S. (2014). Equisetum arvense: Ethanopharmacological and Phytochemical review with reference to osteoporosis Single Enzyme Nanoparticles for Application in Humification and Carbon Sequestration Process View project Phytotherapeutics View project. 1(4). 131–141. https://www.researchgate.net/publication/278585309
  • Referans3 Baytop. T. (1984). Türkiye’de Bitkiler ile Tedavi (geçmişte ve bugün). İstanbul Üniversitesi Yayınları. 3255. 3255.
  • Referans4 Čanadanović-Brunet. J. M.. Ćetković. G. S.. Djilas. S. M.. Tumbas. V. T.. Savatović. S. S.. Mandić. A. I.. Markov. S. L.. & Cvetković. D. D. (2009). Radical scavenging and antimicrobial activity of horsetail (Equisetum arvense L.) extracts. International Journal of Food Science and Technology. 44(2). 269–278. https://doi.org/10.1111/j.1365-2621.2007.01680.x
  • Referans5 Carneiro. D.. Tresvenzol. L.. PCBV. J.. & LC. C. (2013). Equisetum arvense: scientific evidences for clinical use. International Journal of Biology. Pharmacy and Allied Sciences. 2(4). 1579–1596.
  • Referans6 Cetojević-Simin. D. D.. Canadanović-Brunet. J. M.. Bogdanović. G. M.. Djilas. S. M.. Cetković. G. S.. Tumbas. V. T.. & Stojiljković. B. T. (2010). Antioxidative and antiproliferative activities of different horsetail (Equisetum arvense L.) extracts. Journal of Medicinal Food. 13(2). 452–459. https://doi.org/10.1089/jmf.2008.0159
  • Referans7 Garcia. D.. Garcia-Cela. E.. Ramos. A. J.. Sanchis. V.. & Marín. S. (2011). Mould growth and mycotoxin production as affected by Equisetum arvense and Stevia rebaudiana extracts. Food Control. 22(8). 1378–1384. https://doi.org/10.1016/j.foodcont.2011.02.016
  • Referans8 Garcia. D.. Ramos. A. J.. Sanchis. V.. & Marín. S. (2012). Effect of Equisetum arvense and Stevia rebaudiana extracts on growth and mycotoxin production by Aspergillus flavus and Fusarium verticillioides in maize seeds as affected by water activity. International Journal of Food Microbiology. 153(1–2). 21–27. https://doi.org/10.1016/j.ijfoodmicro.2011.10.010
  • Referans9 Gründemann. C.. Lengen. K.. Sauer. B.. Garcia-Käufer. M.. Zehl. M.. & Huber. R. (2014). Equisetum arvense (common horsetail) modulates the function of inflammatory immunocompetent cells. BMC Complementary and Alternative Medicine. 14(1). 4–13. https://doi.org/10.1186/1472-6882-14-283
  • Referans10 Jinous Asgarpanah. (2012). Phytochemistry and pharmacological properties of Equisetum arvense L. Journal of Medicinal Plants Research. 6(21). 3689–3693. https://doi.org/10.5897/jmpr12.234
  • Referans11 Kelimeler. A. (2014). Kelkit (Gümüşhane) Aktarlarında Satılan Tıbbi Bitkilerin Etnobotanik Özellikleri. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi. 18(3). 60–80. https://doi.org/10.19113/sdufbed.49314
  • Referans12 Melİkoğlu. G.. Kurtoğlu. S.. & Kültür. Ş. (2015). Türkiye’de astım tedavisinde geleneksel olarak kullanılan Bitkiler. Marmara Pharmaceutical Journal. 19(1). 1–11. https://doi.org/10.12991/mpj.2015198604
  • Referans13 Mimica-Dukic. N.. Simin. N.. Cvejic. J.. Jovin. E.. Orcic. D.. & Bozin. B. (2008). Phenolic compounds in field horsetail (Equisetum arvense L.) as natural antioxidants. Molecules. 13(7). 1455–1464. https://doi.org/10.3390/molecules13071455
  • Referans14 Nagai. T.. Myoda. T.. & Nagashima. T. (2005). Antioxidative activities of water extract and ethanol extract from field horsetail (tsukushi) Equisetum arvense L. Food Chemistry. 91(3). 389–394. https://doi.org/10.1016/j.foodchem.2004.04.016
  • Referans15 Pallag. A.. Filip. G. A.. Olteanu. D.. Clichici. S.. Baldea. I.. Jurca. T.. Micle. O.. Vicas. L.. Marian. E.. Soriţău. O.. Cenariu. M.. & Mureşan. M. (2018). Equisetum arvense L. extract induces antibacterial activity and modulates oxidative stress. inflammation. and apoptosis in endothelial vascular cells exposed to hyperosmotic stress. Oxidative Medicine and Cellular Longevity. 2018. https://doi.org/10.1155/2018/3060525
  • Referans16 Zhang. H.. Li. N.. Li. K.. & Li. P. (2015). Effect of ethanol root extract of equisetum arvense (L) on urinary bladder activity in rats and analysis of principal plant constituents. Tropical Journal of Pharmaceutical Research. 14(8). 1451–1458. https://doi.org/10.4314/tjpr.v14i8.18

Determination of Phenolic Compound Composition of Water and Ethanol Extracts of Horsetail (Equisetum arvense)

Yıl 2021, Cilt 2, Sayı 2, 3 - 9, 19.05.2021

Öz

Equisetum arvense, commonly known as horsetail, is generally used in traditional medicine as a therapeutic and pain reliever in wound, rheumatism, tuberculosis, skin diseases, hair loss, prostate, asthma, and urinary system diseases. Equisetum arvense contains many phenolic components. These components show high antioxidant activity.
The aim of this study is to determine the phenolic compound composition of water and ethanol extracts of Equisetum arvense by High Performance Liquid Chromatography Equipped with Diode Array Detector (HPLC-DAD) which is sold by herbalists at the local markets and used for treatment in folk medicine.
As a result of our study, the phenolic components of horsetail are found as 4-hydroxybenzoic acid (1.535 µg/kg DW), rutin (14.383 µg/kg DW), salicylic acid (9.639 µg/kg DW), vanillic acid (2.32 µg/kg DW) and chicoric acid (21.313 µg/kg DW) in water extract. Ethanol extract contains rutin (6.23695 µg/kg DW), quercetin (32.9995 µg/kg DW), gallic acid (0.95 µg/kg DW.), epicatechin (24.97 µg/kg DW), p-coumaric acid (5.97495 µg/kg DW), chicoric acid (39.98495 µg/kg DW), and cinnamic acid (1.7695 µg/kg DW).

Kaynakça

  • Referans1 Al-Snafi. P. D. A. E. (2017). The pharmacology of Equisetum arvense- A review. IOSR Journal of Pharmacy (IOSRPHR). 07(02). 31–42. https://doi.org/10.9790/3013-0702013142
  • Referans2 Badole. S. (2014). Equisetum arvense: Ethanopharmacological and Phytochemical review with reference to osteoporosis Single Enzyme Nanoparticles for Application in Humification and Carbon Sequestration Process View project Phytotherapeutics View project. 1(4). 131–141. https://www.researchgate.net/publication/278585309
  • Referans3 Baytop. T. (1984). Türkiye’de Bitkiler ile Tedavi (geçmişte ve bugün). İstanbul Üniversitesi Yayınları. 3255. 3255.
  • Referans4 Čanadanović-Brunet. J. M.. Ćetković. G. S.. Djilas. S. M.. Tumbas. V. T.. Savatović. S. S.. Mandić. A. I.. Markov. S. L.. & Cvetković. D. D. (2009). Radical scavenging and antimicrobial activity of horsetail (Equisetum arvense L.) extracts. International Journal of Food Science and Technology. 44(2). 269–278. https://doi.org/10.1111/j.1365-2621.2007.01680.x
  • Referans5 Carneiro. D.. Tresvenzol. L.. PCBV. J.. & LC. C. (2013). Equisetum arvense: scientific evidences for clinical use. International Journal of Biology. Pharmacy and Allied Sciences. 2(4). 1579–1596.
  • Referans6 Cetojević-Simin. D. D.. Canadanović-Brunet. J. M.. Bogdanović. G. M.. Djilas. S. M.. Cetković. G. S.. Tumbas. V. T.. & Stojiljković. B. T. (2010). Antioxidative and antiproliferative activities of different horsetail (Equisetum arvense L.) extracts. Journal of Medicinal Food. 13(2). 452–459. https://doi.org/10.1089/jmf.2008.0159
  • Referans7 Garcia. D.. Garcia-Cela. E.. Ramos. A. J.. Sanchis. V.. & Marín. S. (2011). Mould growth and mycotoxin production as affected by Equisetum arvense and Stevia rebaudiana extracts. Food Control. 22(8). 1378–1384. https://doi.org/10.1016/j.foodcont.2011.02.016
  • Referans8 Garcia. D.. Ramos. A. J.. Sanchis. V.. & Marín. S. (2012). Effect of Equisetum arvense and Stevia rebaudiana extracts on growth and mycotoxin production by Aspergillus flavus and Fusarium verticillioides in maize seeds as affected by water activity. International Journal of Food Microbiology. 153(1–2). 21–27. https://doi.org/10.1016/j.ijfoodmicro.2011.10.010
  • Referans9 Gründemann. C.. Lengen. K.. Sauer. B.. Garcia-Käufer. M.. Zehl. M.. & Huber. R. (2014). Equisetum arvense (common horsetail) modulates the function of inflammatory immunocompetent cells. BMC Complementary and Alternative Medicine. 14(1). 4–13. https://doi.org/10.1186/1472-6882-14-283
  • Referans10 Jinous Asgarpanah. (2012). Phytochemistry and pharmacological properties of Equisetum arvense L. Journal of Medicinal Plants Research. 6(21). 3689–3693. https://doi.org/10.5897/jmpr12.234
  • Referans11 Kelimeler. A. (2014). Kelkit (Gümüşhane) Aktarlarında Satılan Tıbbi Bitkilerin Etnobotanik Özellikleri. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi. 18(3). 60–80. https://doi.org/10.19113/sdufbed.49314
  • Referans12 Melİkoğlu. G.. Kurtoğlu. S.. & Kültür. Ş. (2015). Türkiye’de astım tedavisinde geleneksel olarak kullanılan Bitkiler. Marmara Pharmaceutical Journal. 19(1). 1–11. https://doi.org/10.12991/mpj.2015198604
  • Referans13 Mimica-Dukic. N.. Simin. N.. Cvejic. J.. Jovin. E.. Orcic. D.. & Bozin. B. (2008). Phenolic compounds in field horsetail (Equisetum arvense L.) as natural antioxidants. Molecules. 13(7). 1455–1464. https://doi.org/10.3390/molecules13071455
  • Referans14 Nagai. T.. Myoda. T.. & Nagashima. T. (2005). Antioxidative activities of water extract and ethanol extract from field horsetail (tsukushi) Equisetum arvense L. Food Chemistry. 91(3). 389–394. https://doi.org/10.1016/j.foodchem.2004.04.016
  • Referans15 Pallag. A.. Filip. G. A.. Olteanu. D.. Clichici. S.. Baldea. I.. Jurca. T.. Micle. O.. Vicas. L.. Marian. E.. Soriţău. O.. Cenariu. M.. & Mureşan. M. (2018). Equisetum arvense L. extract induces antibacterial activity and modulates oxidative stress. inflammation. and apoptosis in endothelial vascular cells exposed to hyperosmotic stress. Oxidative Medicine and Cellular Longevity. 2018. https://doi.org/10.1155/2018/3060525
  • Referans16 Zhang. H.. Li. N.. Li. K.. & Li. P. (2015). Effect of ethanol root extract of equisetum arvense (L) on urinary bladder activity in rats and analysis of principal plant constituents. Tropical Journal of Pharmaceutical Research. 14(8). 1451–1458. https://doi.org/10.4314/tjpr.v14i8.18

Ayrıntılar

Birincil Dil İngilizce
Konular Eczacılık
Bölüm Araştırma Makaleleri
Yazarlar

Sibel ATASEVEN (Sorumlu Yazar)
SAĞLIK BİLİMLERİ ÜNİVERSİTESİ, HAMİDİYE SAĞLIK BİLİMLERİ ENSTİTÜSÜ, GELENEKSEL VE TAMAMLAYICI TIP ANABİLİM DALI (DİSİPLİNLERARASI), GELENEKSEL VE TAMAMLAYICI TIP (YL) (TEZLİ)
0000-0002-2500-1849
Türkiye


Duygu MISIRLI
SAĞLIK BİLİMLERİ ÜNİVERSİTESİ, HAMİDİYE ECZACILIK FAKÜLTESİ, HAMİDİYE ECZACILIK PR.
0000-0002-3581-4368
Türkiye


Fatıma UZAR
SAĞLIK BİLİMLERİ ÜNİVERSİTESİ, HAMİDİYE SAĞLIK BİLİMLERİ ENSTİTÜSÜ, FİTOTERAPİ (DR)
0000-0002-8580-3712
Türkiye


Nuran Neslinur TÜRKAN Bu kişi benim
SAĞLIK BİLİMLERİ ÜNİVERSİTESİ, HAMİDİYE SAĞLIK BİLİMLERİ ENSTİTÜSÜ, GELENEKSEL VE TAMAMLAYICI TIP ANABİLİM DALI (DİSİPLİNLERARASI), GELENEKSEL VE TAMAMLAYICI TIP (YL) (TEZLİ)
0000-0003-2549-9242
Türkiye


Mahfuz ELMASTAŞ
SAĞLIK BİLİMLERİ ÜNİVERSİTESİ, HAMİDİYE ECZACILIK FAKÜLTESİ, HAMİDİYE ECZACILIK PR.
0000-0002-7149-7427
Türkiye

Destekleyen Kurum Sağlık Bilimleri Üniversitesi Bilimsel Araştırma Projeleri Birimi
Proje Numarası 2020/086
Yayımlanma Tarihi 19 Mayıs 2021
Yayınlandığı Sayı Yıl 2021, Cilt 2, Sayı 2

Kaynak Göster

APA Ataseven, S. , Mısırlı, D. , Uzar, F. , Türkan, N. N. & Elmastaş, M. (2021). Determination of Phenolic Compound Composition of Water and Ethanol Extracts of Horsetail (Equisetum arvense) . Bütünleyici ve Anadolu Tıbbı Dergisi , 2 (2) , 3-9 . Retrieved from https://dergipark.org.tr/tr/pub/batd/issue/62370/925331

Bütünleyici ve Anadolu Tıbbı Dergisi

Journal of Integrative and Anatolian Medicine