Investigation of antioxidant, enzyme inhibitory effects and total secondary metabolite quantification of Ornithogalum nutans L. methanol

Öz

This study was designed to determine the antioxidant and enzyme inhibitory activities of methanol extracts of different parts (aerial and bulb) of Ornithogalum nutans as well as to investigate the total phenolic and flavonoid amounts. While the total phenolic content of the extracts varied between 1.43±0.01/3.05±0.04 mg GAE/g of extract equivalent, the total flavonoid amount varied between 0.33±0.01/1.79±0.02 mg QE/g. Antioxidant activities were determined by DPPH, ABTS, ß-Carotene/Linoleic acid, CUPRAC, and FRAP methods. The antioxidant activity studies determined that the aerial methanol extract exhibited a higher activity than the bulb. Acetylcholinesterase and tyrosinase enzyme inhibitory activities of the extracts were determined, and the extracts showed lower activity than galantamine (89.41±0.05 %) and kojic acid (89.41±0.05 %) used as standard. The results reveal the potential of O. nutans extracts to be used in pharmacological studies in the pharmaceutical and food industries.

Anahtar Kelimeler

• Ornithogalum nutans
• antioxidant
• acetylcholinesterase
• tyrosinase
• secondary metabolites

Ornithogalum nutans L. Metanol ekstraktlarının antioksidan, enzim inhibitör Etkileri, total sekonder metabolit miktar tayininin araştırılması

Öz

Bu çalışma Ornithogalum nutans türünün farklı kısımlarının (yerüstü ve yeraltı) metanol ekstraktlarının antioksidan ve enzim inhibitör aktivitelerinin belirlenmesinin yanı sıra total fenolik ve flavonoid miktarlarının araştırılması için tasarlanmıştır. Ekstraktların total fenolik miktarı 1.43±0.01/3.05±0.04 mg GAE/g ekstrakt eşdeğeri arasında değişirken, total flavonoid miktarı ise 0.33±0.01/1.79±0.02 mg QE/g arasında değişmektedir. Antioksidan aktiviteleri DPPH, ABTS, ß-Karoten/Linoleik asit, CUPRAC ve FRAP yöntemleri ile belirlemiştir. Yapılan antioksidan aktivite çalışmalarında yerüstü metanol ekstraktının, yeraltına göre daha yüksek bir aktivite sergilediği tespit edilmiştir. Ekstraktların asetilkolinesteraz ve tirozinaz enzim inhibitör aktiviteleri belirlenmiştir ve ekstraktların standart olarak kullanılan galantamin (89.41±0.05 %) ve kojik asite (89.41±0.05 %) göre daha düşük bir aktivite sergilemişlerdir. Sonuçlar, O. nutans ekstraktlarının farmakolojik çalışmalarda ilaç ve gıda endüstrilerinde kullanılabilme potansiyellerini ortaya koymaktadır.

Anahtar Kelimeler

• Ornithogalum nutans
• antioksidan
• asetilkolinesteraz
• tirozinaz
• sekonder metabolit

Kaynakça

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