Bazı bitki büyümesini teşvik eden rizobakterilerin (PGPR) buğday ve mısır tohumlarının gelişimi üzerindeki sinerjik etkisinin in vitro koşullarda belirlenmesi

Öz

Yapılan bu çalışmada yerel kaynaklardan izole edilmiş, PGPR (Plant Growth Promoting Rhizobacteria) özellikleri belirlenmiş 9 Bacillus suşunun buğday, 5 Bacillus, 1 Pseudomonas ve 1 Stenotrophomonas suşunun ise mısır tohumlarının gelişimi üzerindeki sinerjik etkileri incelenmiştir. Bakteri suşlarının her birinden hazırlanan biyoinokülantların (108 kob/ml) tek tek suşlar ve eşit hacimlerde karıştırılmasıyla oluşturulan ikili ve üçlü suş kombinasyonlar şeklinde steril buğday tohumları ile muamele edilip saksılara yerleştirilmiştir. Steril mısır tohumları ise saksılara yerleştirildikten sonra biyoinokülantların tek tek suşlar ve eşit hacimlerde karıştırılmış ikili, üçlü ve dörtlü suş kombinasyonlar şeklinde inoküle edilmiştir. Denemeler üçer tekrarlı olacak şekilde tasarlanmıştır. Kontrollü koşullar altında buğday ve mısır tohumlarının gelişim denemeleri sırasıyla 30 ve 45 gün boyunca devam edilmiştir. Tohumların gelişimi gövde uzunluğu, gövde yaş, gövde kuru, kök yaş ve kök kuru ağırlıkları açısından kontrol grubu ile istatistiksel olarak karşılaştırıldığında buğday için (B. subtilis B.3.P.5 + B. subtilis 1.19 + B. subtilis 36.5) ve (B. subtilis B.3.P.5 + B. subtilis 36.5 + B. simplex B.1.2.k), mısır için ise (B. subtilis 1.19 + B. simplex B.1.2.k + B. megaterium 42.3) ve (B. megaterium 42.3 + B. subtilis 36.5 + S. rhizophila 118.1 + P. chlororaphis P-102-b-a) kombinasyonlarının yüksek sinerjik etkiye sahip olduğu belirlenmiştir.

Anahtar Kelimeler

• PGPR
• sinerjik etki
• buğday
• mısır
• tohum gelişimi

Determination of the synergistic effect of some plant growth promoting rhizobacteria (PGPR) on the development of wheat and maize seeds in vitro conditions

Öz

In this study, the synergistic effects of 9 Bacillus strains isolated from local sources with PGPR (Plant Growth Promoting Rhizobacteria) properties on the development of wheat seeds, 5 Bacillus, 1 Pseudomonas and 1 Stenotrophomonas strains on the growth of maize seeds were investigated. Bioinoculants (108 cfu/ml) prepared from each of the bacterial strains in the form of single combinations, and double and triple strain combinations created by mixing equal volumes were treated with sterile wheat seeds and placed in pots. After sterile maize seeds were placed in pots, bioinoculants were inoculated into seeds as single strains and in equal volumes of mixed double, triple and quadruple strain combinations. Trials were designed to be repeated in triplicate. Growth trials of wheat and maize seeds under controlled conditions were continued for 30 and 45 days, respectively. When the development of seeds was statistically compared with the control group in terms of shoot length, shoot fresh, shoot dry, root fresh and root dry weights, it was determined that combinations of (B. subtilis B.3.P.5 + B. subtilis 1.19 + B. subtilis 36.5) and (B. subtilis B.3.P.5 + B. subtilis 36.5 + B. simplex B.1.2.k) for wheat, and (B. subtilis 1.19 + B. simplex B.1.2.k + B. megaterium 42.3) and (B. megaterium 42.3 + B. subtilis 36.5 + S. rhizophila 118.1 + P. chlororaphis P-102-b-a) for maize had a high synergistic effect.

Anahtar Kelimeler

• PGPR
• synergistic effect
• wheat
• maize
• seed development

Kaynakça

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