Synthesis and characterization of gelatin-based quaternizable hydrogels

Öz

Gelatin, a water-soluble natural polymer with excellent film-forming properties, exhibits high biocompatibility due to its amino acid composition, which closely resembles that of proteins. However, gelatin has poor mechanical properties and poses a risk of bacterial infection when films are composed solely of gelatin. In this study, gelatin-based crosslinked polymers with quaternary amine groups, exhibiting potential antibacterial properties, were developed. To achieve this, gelatin was first modified with methacrylate via an isocyanate-amine reaction, and the resulting material was characterized using Fourier-transform infrared spectroscopy (FTIR) and nuclear magnetic resonance spectroscopy (NMR). Hydrogels were successfully synthesized by photopolymerization of gelatin methacryloyl, with a tertiary amine-containing monomer and a four-arm crosslinker, and characterized using FTIR, scanning electron microscope (SEM), and thermogravimetric analysis (TGA). Subsequently, the hydrogel was prepared as a film on a glass surface, and quaternization of the tertiary amine groups imparted polycationic properties to the hydrogel coatings, enabling further investigation into their antibacterial potential.

Anahtar Kelimeler

• gelatin
• photopolymerization
• hydrogels
• quaternization
• isocyanate-amine

Jelatin bazlı kuaternize edilebilir hidrojellerin sentezi ve karakterizasyonu

Öz

Üstün film oluşturma özelliklerine sahip, suda çözünebilen doğal bir polimer olan jelatin, proteinlere benzer amino asit bileşimi sayesinde yüksek biyouyumluluk sergilemektedir. Ancak jelatinin mekanik özellikleri zayıftır ve yalnızca jelatinden oluşan filmler, bakteriyel enfeksiyon riski taşımaktadır. Bu çalışmada, potansiyel antibakteriyel özelliklere sahip kuaterner amin grupları içeren jelatin bazlı çapraz bağlı polimerlerin geliştirilmesi amaçlanmıştır. Bunun için önce jelatin izosiyanat-amin reaksiyonu kullanarak metakrilat ile modifiye edilmiş ve ardından fourier dönüşümlü kızılötesi spektroskopisi (FTIR) ve nükleer manyetik rezonans spektroskopisi HNMR ile yapısı aydınlatılmıştır. Jelatin metakriloilin tersiyer amin içeren monomer ve dört kollu bir çapraz bağlayıcı monomer ile fotopolimerleşmesi sonucu hidrojeller elde edilmiş ve FTIR, taramalı elektron mikroskobu (SEM) ve Termogravimetrik Analiz (TGA) teknikleri ile karakterize edilmiştir. Ardından, hidrojeller cam yüzeyde bir film olarak da hazırlanmış ve üçüncül amin gruplarının kuaternizasyonu ile hidrojel kaplamalara polikatyonik özellikler kazandırılarak, antibakteriyel potansiyel barındıran yeni malzemeler elde edilmiştir.

Anahtar Kelimeler

• jelatin
• fotopolimerleşme
• hidrojeller
• kuaternizasyon
• izosiyanat-amin

Kaynakça

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