The genotoxic and biochemical effects of wastewater samples from a fat plant in Erzurum

Öz

Toxicological effects of effluent exposure may be acute or chronic, and can occur at all levels of biological organization, from the molecular to the ecosystem level. The most common strategy to investigate potential adverse effects and toxicological modes of action following effluent exposure is the use of biological tests. The frequencies of sister chromatid exchanges (SCEs) and superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) enzyme activities were assessed on human blood cultures exposed to wastewater samples (WWS). The water samples were taken from a local fat plant in Ilıca region (Erzurum). Metal ions (Fe, Cu, Mn, Zn, Pb, Cd) were measured by an Atomic Absorption Spectrophotometer (AAS). According to our results, SCE rates increased significantly (P<0.0.5) in dose-responded manner after treatments with WWS. And there was a significant positive correlation between SCE frequencies and WWS concentrations (r = 0.98). On the other hand, SOD, CAT and GSH-Px enzyme activities decreased in erythrocytes. These results reveal that high level of heavy metal content (especially lead and cadmium) of WWS caused genotoxic damage by oxidative stress. Thus, this could lead to adverse health effects for exposed human and animal populations

Anahtar Kelimeler

• Genotoxicity, oxidative stress, human blood, wastewater, fat plant

Erzurum’daki bir yağ fabrikasından alınan atıksu örneklerinin genotoksik ve biyokimyasal etkileri

Öz

Atık maruziyetinin toksikolojik etkileri akut ya da kronik olabilir ve moleküler düzeyden ekosistem düzeyine kadar her biyolojik organizasyonun tüm aşamasında ortaya çıkabilir. Atık maruziyetini takiben bu atığın toksikolojik etki yolunu ve olumsuz etkilerini araştırmanın en yaygın stratejisi biyolojik testler kullanmaktır. Kardeş kromozom değişim frekansları (SCEs) ve süperoksit dismutaz (SOD) katalaz (CAT) ve glutatyon peroksidaz (GSH-Px) enzim aktiviteleri atık su örneklerine (WWS) maruz kalmış insan kanı kültürlerinde araştırıldı. Su örnekleri Ilıca (Erzurum) yöresinde bulunan bir yağ fabrikasından alındı. Metal iyonları (Fe, Cu, Mn, Zn, Pb, Cd) Atomik Absorpsiyon Spektrofotometresi (AAS) ile ölçüldü. Sonuçlarımıza göre WWS uygulamasından sonra SCE frekansı önemli ölçüde yükseldi (P

Anahtar Kelimeler

• Genotoksisite, oksidatif stres, insan kanı, atıksu, yağ fabrikası

Kaynakça

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