Investigation of anticancer/wound healing activity of donkey milk and its some components in vitro

Öz

In this study, it was aimed to investigate the anticancer and wound healing potential of donkey milk, casein and whey protein in vitro. First of all, casein and whey proteins were obtained by changing the pH value of donkey milk. The effect of donkey milk, casein and whey protein on anticancer activity on human prostate cancer cell line (PC-3) was investigated using MTT cell proliferation assay in vitro and no anticancer activity was detected. In MTT experiments, it was observed that casein protein caused cell proliferation of 140%. Accordingly, cell migration and wound healing effects of these proteins were investigated by applying scratch test on human fibroblast cell line (BJ). Donkey milk, casein and whey protein (3200 µg / ml) caused a significant increase in cell migration and in vitro wound healing rate in comparison to the control group. With this study, it was shown that donkey milk and other proteins in it did not show anticancer activity in vitro but caused cell proliferation and could be used for wound healing purpose.

Anahtar Kelimeler

• Donkey milk,casein,whey protein,anticancer,wound healing

Eşek sütü ve bazı bileşenlerinin in vitro ortamda antikanser/yara iyileştirme aktivitesinin incelenmesi

Öz

Bu çalışmada, eşek sütü, kazein ve kesik süt suyu proteininin in vitro ortamda antikanser ve yara iyileştirme potansiyelinin araştırılması amaçlanmıştır. Öncelikle eşek sütünün pH değeri değiştirilerek kazein ve kesik süt suyu proteinleri elde edilmiştir. Eşek sütü, kazein ve kesik süt suyu proteininin in vitro ortamda insan prostat kanseri hücre hattı (PC-3) üzerindeki antikanser aktivitesi MTT hücre proliferasyon testi kullanılarak incelenmiş olup antikanser aktivite bulunamamıştır. MTT denemelerinde özellikle kazein proteininin %140 hücre proliferasyonuna sebep olduğu gözlenmiştir. Bu doğrultuda, insan fibroblast hücre hattı (BJ) üzerinde çizik testi uygulanarak bu proteinlerin hücre migrasyonu ve yara iyileştirici etkileri incelenmiştir. Eşek sütü, kazein ve kesik süt suyu proteini (3200 µg/ml) kontrol grubuna kıyasla hücre migrasyonunda ve in vitro yara kapanma hızında anlamlı artışa sebep olmuştur. Bu çalışma ile eşek sütünün ve içerisindeki diğer proteinlerin in vitro ortamda antikanser aktivite göstermeyip hücre proliferasyonuna sebep olduğu ve yara iyileştirme amaçlı kullanılabileceği gösterilmiştir.

Anahtar Kelimeler

• Eşek sütü,kazein,kesik süt suyu proteini,antikanser,yara iyileştirme

Destekleyen Kurum

TÜBİTAK 2209-A Üniversite Öğrencileri Araştırma Projeleri Destekleme Programı

Teşekkür

Bu çalışma TÜBİTAK 2209-A Üniversite Öğrencileri Araştırma Projeleri Destekleme Programı tarafından desteklenmiştir.

Kaynakça

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