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The genotoxic and biochemical effects of wastewater samples from a fat plant in Erzurum

Yıl 2009, Cilt: 11 Sayı: 2, 55 - 64, 01.12.2009

Öz

Toxicological effects of effluent exposure may be acute or chronic, and can occur at all levels of biological organization, from the molecular to the ecosystem level. The most common strategy to investigate potential adverse effects and toxicological modes of action following effluent exposure is the use of biological tests. The frequencies of sister chromatid exchanges (SCEs) and superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) enzyme activities were assessed on human blood cultures exposed to wastewater samples (WWS). The water samples were taken from a local fat plant in Ilıca region (Erzurum). Metal ions (Fe, Cu, Mn, Zn, Pb, Cd) were measured by an Atomic Absorption Spectrophotometer (AAS). According to our results, SCE rates increased significantly (P<0.0.5) in dose-responded manner after treatments with WWS. And there was a significant positive correlation between SCE frequencies and WWS concentrations (r = 0.98). On the other hand, SOD, CAT and GSH-Px enzyme activities decreased in erythrocytes. These results reveal that high level of heavy metal content (especially lead and cadmium) of WWS caused genotoxic damage by oxidative stress. Thus, this could lead to adverse health effects for exposed human and animal populations

Kaynakça

  • [1] Sang, N., Li, G., “Genotoxicity of municipal landfill leachate on root tips of Vicia faba”, Mutation Research, 560, 159–165, (2004).
  • [2] Cerna, M., Hajek, V., Stejskalova, E., Dobia, L., Zudova, Z., Rossner, P., “Environmental genotoxicity monitoring using Salmonella typhimurium strains as indicator system”, Science of the Total Environment, 101, 139–147, (1991).
  • [3] Bakare, A.A., Pandey, A.K., Bajpayee, M., Bhargav, D., Chowdhuri. D.K., Singh. K.P., Murthy, R.C., Dhawan, A., “DNA damage induced in human peripheral blood lymphocytes by industrial solid waste and municipal sludge leachates”, Environmental and Molecular Mutagenesis, 48, 30-37, (2007).
  • [4] Hallenbeck, W.H., “Human health effects of exposure to cadmium”, Experientia Supplement, 50, 131-137, (1986).
  • [5] Snow, E.T., “Metal carcinogenesis: mechanistic implications”, Pharmacology and Therapeutics, 53, 31– 65, (1992).
  • [6] Bolognesi, C., Landini, E., Roggieri, P., Fabbri, R., Viarengo, A., “Genotoxicity biomarkers in the assessment of heavy metal effects in mussels: Experimental studies”, Environmental and Molecular Mutagenesis, 33, 287-292, (1999).
  • [7] Durgo, K., Orešcanin, V., Luliç, S., Kopjar, N., Želježiç, D., Coliç, J.F., “The assessment of genotoxic effects of wastewater from a fertilizer factory”, Journal of Applied Toxicology, 29, 42–51, (2009).
  • [8] Valko, M., Rhodes, C.J., Moncol, J., Izakovic, M., Mazur, M., “Free radicals, metals and antioxidants in oxidative stress-induced cancer”, Chemico-Biological Interactions, 106, 1–40, (2006).
  • [9] Gana, J.M., Ordonez, R., Zampini, C., Hidalgo, M., Meoni, S., Isla, M.I., “Industrial effluents and surface waters genotoxicity and mutagenicity evaluation of a river of Tucuman, Argentina”, Journal of Hazardous Material, 155, 403-406, (2008).
  • [10] Spronck, J.C., Kirkland, J.B., “Niacin deficiency increases spontaneous and etoposide induced chromosomal instability in rat bone marrow cells in vivo”, Mutation Research, 508, 83-97, (2002).
  • [11] Şişman, T., Đncekara, Ü., Yıldız, Y.Ş., “Determination of acute and early life stage toxicity of fat-plant effluent using zebrafish (Danio rerio)”, Environmental Toxicology, 23: 480-486, (2008).
  • [12] APHA, AWWA, WPCF, “Standard methods for examination of water and wastewater”, 20th ed. Washington, DC: American Public Health Association, (1998).
  • [13] Evans, H.J., O’Riordan, M.L., “Human peripheral blood lymphocytes for the analysis of chromosome aberrations in mutagen tests”, Mutation Research, 31, 135-148, (1975).
  • [14] Misra, H.P. Fridovich, K., “The generation of superoxide radical during the autooxidation of hemoglobin”, Journal of Biological Chemistry, 247, 6960–6962, (1972).
  • [15] Aebi, H., “Catalase in vitro”, pp. 1-126. In: Packer L. (ed), Methods in Enzymology. Vol. 105, Academic Press, Orlando. (1984).
  • [16] Carlberg, I., Mannervik, B., “Purification and characterization of the flavoenzyme glutathione reductase from rat liver Journal of Biological Chemistry, 250, 5475– 5480, (1972).
  • [17] Shaik, P.A., Sankar, S., Reddy, S.C., Das, P.G., Jamil, K., “Lead-induced genotoxicity in lymphocytes from peripheral blood samples of humans: in vitro studies”, Drug and Chemical Toxicology, 29, 111-124, (2006).
  • [18] Pinheiro, M.C., Macchi, B.M., Vieira, J.L., Oikawa, T., Amoras, W.W., Guimarães, G.A., Costa, C.A., Crespo-López, M.E., Herculano, A.M., Silveira, L.C. and do Nascimento, J.L., “Mercury exposure and antioxidant defenses in women: a comparative study in the Amazon”, Environmental Research, 107, 53- 59, (2008).
  • [19] Khan, D., Qayyum, S., Saleem, S., Khan, F., “Lead-induced oxidative stress adversely affects health of the occupational workers”, Toxicology and Industrial Health, 24, 611-618, (2008).
  • [20] Chater, S., Douki, T., Garrel, C., Favier, A., Sakly, M., Abdelmelek, H.C.R., “Cadmium-induced oxidative stress and DNA damage in kidney of pregnant female rats”, Comptes Rendus Biologies, 331, 426-432, (2008).
  • [21] Moriwaki, H., Osborne, M.R., Phillips, D.H., “Effects of mixing metal ions on oxidative DNA damage mediated by a Fenton-type reduction”, Toxicology In Vitro, 22, 36-44, (2007).
  • [22] Nagy, E., Johansson, C., Zeisig, M., Moller, M., “Oxidative stress and DNA damage caused by the urban air pollutant 3-NBA and its isomer 2-NBA in human lung cells analyzed with three independent methods”, Journal of Chromatography. B: Analytical Technologies in the Biomedical and Life Sciences, 827, 94–103, (2005).
  • [23] Kim H., Oh E., Im H., Mun J., Yang M., Khim J. Y., Lee E., Lim S. H., Kong M. H., Lee M., and Sul D., “Oxidative damages in the DNA, lipids, and proteins of rats exposed to isofluranes and alcohols”, Toxicology, 220, 169-178, (2006).
  • [24] Kasperczyk S, Kasperczyk J, Ostałowska A, Zalejska-Fiolka J, Wielkoszyński T, Swiętochowska E, Birkner E., “The role of the antioxidant enzymes in erythrocytes in the development of arterial hypertension among humans exposed to lead”, Biological Trace Elements Research, 130, 95-106, 2009.
  • [25] Tabrez S, and Ahmad M., “Effect of wastewater intake on antioxidant and marker enzymes of tissue damage in rat tissues: Implications for the use of biochemical markers”, Food and Chemical Toxicology, 47, 2465–2478, 2009.
  • [26] Fatima, R.A., and Ahmad M., “Certain antioxidant enzymes of Allium cepa as biomarkers for the detection of toxic heavy metals in wastewater”, Science of The Total Environment, 346, 256-273, 2005.
  • [27] Labrot, F., Ribera, D., Saint Denis, M., and Narbonne, J.F., “In vitro and in vivo studies of potential biomarkers of lead and uranium contamination: lipid peroxidation, acetylcholinesterase, catalase and glutathione peroxidase activities in three non-mammalian species”, Biomarkers, 1, 21-28, 1996.
  • [28] Bengtsson, A., Lundberg, M., Avila-Carino, J., Jacobsson, G., Holmgren, A., Scheynius, A., “Thiols decrease cytokine levels and down-regulate the expression of CD30 on human allergen-specific T helper (Th) 0 and TH2 cells”, Clinical Experimental Immunology, 123, 350-360, (2001).
  • [29] Rank, J, and Nielsen, M.H., “Genotoxicity testing of wastewater sludge using the Allium cepa anaphase-telophase chromosome aberration assay”, Mutation Research, 418, 113-119, 1998.
  • [30] Dizer, H., Wittekindt, E., Fischer, B., and Hansen, P.D., “The cytotoxic and genotoxic potential of surface water and wastewater effluents as determined by bioluminescence, umu-assays and selected biomarkers”, Chemosphere, 46, 225- 233, 2002.
  • [31] Mao, I.F., Chen, M.L., Lan, C.F., Chang, Y.P., and Chang, S.C., “Mutagenicity determination of the wastewater emitted from petrochemical industry in Taiwan”, Water Air & Soil Pollution, 76, 459-466, 1994.
  • [32] Malik, A., and Ahmad, A., “Genotoxicity of some wastewaters in India”, Environmental Toxicology, 10, 287-293, 2006.
  • [33] Barsiene, J., Andreikenaite, L., Vosyliene, M.Z., and Milukaite, A., “Genotoxicity and Immunotoxicity of Wastewater Effluents Discharged from Vilnius Wastewater Treatment Plant” Acta Zoologica Lituanica, 19, 188-196, 2009.
  • [34] Krishnamurthi, K., Saravana Devi, S., Hengstler, J.G., Hermes, M., Kumar, K., Dutta, D., Muhil Vannan, S., Subin, T.S., Yadav, R.R., and Chakrabarti, T., “Genotoxicity of sludges, wastewater and effluents from three different industries”, Archives of Toxicology, 82, 965-971, 2008.

Erzurum’daki bir yağ fabrikasından alınan atıksu örneklerinin genotoksik ve biyokimyasal etkileri

Yıl 2009, Cilt: 11 Sayı: 2, 55 - 64, 01.12.2009

Öz

Atık maruziyetinin toksikolojik etkileri akut ya da kronik olabilir ve moleküler düzeyden ekosistem düzeyine kadar her biyolojik organizasyonun tüm aşamasında ortaya çıkabilir. Atık maruziyetini takiben bu atığın toksikolojik etki yolunu ve olumsuz etkilerini araştırmanın en yaygın stratejisi biyolojik testler kullanmaktır. Kardeş kromozom değişim frekansları (SCEs) ve süperoksit dismutaz (SOD) katalaz (CAT) ve glutatyon peroksidaz (GSH-Px) enzim aktiviteleri atık su örneklerine (WWS) maruz kalmış insan kanı kültürlerinde araştırıldı. Su örnekleri Ilıca (Erzurum) yöresinde bulunan bir yağ fabrikasından alındı. Metal iyonları (Fe, Cu, Mn, Zn, Pb, Cd) Atomik Absorpsiyon Spektrofotometresi (AAS) ile ölçüldü. Sonuçlarımıza göre WWS uygulamasından sonra SCE frekansı önemli ölçüde yükseldi (P

Kaynakça

  • [1] Sang, N., Li, G., “Genotoxicity of municipal landfill leachate on root tips of Vicia faba”, Mutation Research, 560, 159–165, (2004).
  • [2] Cerna, M., Hajek, V., Stejskalova, E., Dobia, L., Zudova, Z., Rossner, P., “Environmental genotoxicity monitoring using Salmonella typhimurium strains as indicator system”, Science of the Total Environment, 101, 139–147, (1991).
  • [3] Bakare, A.A., Pandey, A.K., Bajpayee, M., Bhargav, D., Chowdhuri. D.K., Singh. K.P., Murthy, R.C., Dhawan, A., “DNA damage induced in human peripheral blood lymphocytes by industrial solid waste and municipal sludge leachates”, Environmental and Molecular Mutagenesis, 48, 30-37, (2007).
  • [4] Hallenbeck, W.H., “Human health effects of exposure to cadmium”, Experientia Supplement, 50, 131-137, (1986).
  • [5] Snow, E.T., “Metal carcinogenesis: mechanistic implications”, Pharmacology and Therapeutics, 53, 31– 65, (1992).
  • [6] Bolognesi, C., Landini, E., Roggieri, P., Fabbri, R., Viarengo, A., “Genotoxicity biomarkers in the assessment of heavy metal effects in mussels: Experimental studies”, Environmental and Molecular Mutagenesis, 33, 287-292, (1999).
  • [7] Durgo, K., Orešcanin, V., Luliç, S., Kopjar, N., Želježiç, D., Coliç, J.F., “The assessment of genotoxic effects of wastewater from a fertilizer factory”, Journal of Applied Toxicology, 29, 42–51, (2009).
  • [8] Valko, M., Rhodes, C.J., Moncol, J., Izakovic, M., Mazur, M., “Free radicals, metals and antioxidants in oxidative stress-induced cancer”, Chemico-Biological Interactions, 106, 1–40, (2006).
  • [9] Gana, J.M., Ordonez, R., Zampini, C., Hidalgo, M., Meoni, S., Isla, M.I., “Industrial effluents and surface waters genotoxicity and mutagenicity evaluation of a river of Tucuman, Argentina”, Journal of Hazardous Material, 155, 403-406, (2008).
  • [10] Spronck, J.C., Kirkland, J.B., “Niacin deficiency increases spontaneous and etoposide induced chromosomal instability in rat bone marrow cells in vivo”, Mutation Research, 508, 83-97, (2002).
  • [11] Şişman, T., Đncekara, Ü., Yıldız, Y.Ş., “Determination of acute and early life stage toxicity of fat-plant effluent using zebrafish (Danio rerio)”, Environmental Toxicology, 23: 480-486, (2008).
  • [12] APHA, AWWA, WPCF, “Standard methods for examination of water and wastewater”, 20th ed. Washington, DC: American Public Health Association, (1998).
  • [13] Evans, H.J., O’Riordan, M.L., “Human peripheral blood lymphocytes for the analysis of chromosome aberrations in mutagen tests”, Mutation Research, 31, 135-148, (1975).
  • [14] Misra, H.P. Fridovich, K., “The generation of superoxide radical during the autooxidation of hemoglobin”, Journal of Biological Chemistry, 247, 6960–6962, (1972).
  • [15] Aebi, H., “Catalase in vitro”, pp. 1-126. In: Packer L. (ed), Methods in Enzymology. Vol. 105, Academic Press, Orlando. (1984).
  • [16] Carlberg, I., Mannervik, B., “Purification and characterization of the flavoenzyme glutathione reductase from rat liver Journal of Biological Chemistry, 250, 5475– 5480, (1972).
  • [17] Shaik, P.A., Sankar, S., Reddy, S.C., Das, P.G., Jamil, K., “Lead-induced genotoxicity in lymphocytes from peripheral blood samples of humans: in vitro studies”, Drug and Chemical Toxicology, 29, 111-124, (2006).
  • [18] Pinheiro, M.C., Macchi, B.M., Vieira, J.L., Oikawa, T., Amoras, W.W., Guimarães, G.A., Costa, C.A., Crespo-López, M.E., Herculano, A.M., Silveira, L.C. and do Nascimento, J.L., “Mercury exposure and antioxidant defenses in women: a comparative study in the Amazon”, Environmental Research, 107, 53- 59, (2008).
  • [19] Khan, D., Qayyum, S., Saleem, S., Khan, F., “Lead-induced oxidative stress adversely affects health of the occupational workers”, Toxicology and Industrial Health, 24, 611-618, (2008).
  • [20] Chater, S., Douki, T., Garrel, C., Favier, A., Sakly, M., Abdelmelek, H.C.R., “Cadmium-induced oxidative stress and DNA damage in kidney of pregnant female rats”, Comptes Rendus Biologies, 331, 426-432, (2008).
  • [21] Moriwaki, H., Osborne, M.R., Phillips, D.H., “Effects of mixing metal ions on oxidative DNA damage mediated by a Fenton-type reduction”, Toxicology In Vitro, 22, 36-44, (2007).
  • [22] Nagy, E., Johansson, C., Zeisig, M., Moller, M., “Oxidative stress and DNA damage caused by the urban air pollutant 3-NBA and its isomer 2-NBA in human lung cells analyzed with three independent methods”, Journal of Chromatography. B: Analytical Technologies in the Biomedical and Life Sciences, 827, 94–103, (2005).
  • [23] Kim H., Oh E., Im H., Mun J., Yang M., Khim J. Y., Lee E., Lim S. H., Kong M. H., Lee M., and Sul D., “Oxidative damages in the DNA, lipids, and proteins of rats exposed to isofluranes and alcohols”, Toxicology, 220, 169-178, (2006).
  • [24] Kasperczyk S, Kasperczyk J, Ostałowska A, Zalejska-Fiolka J, Wielkoszyński T, Swiętochowska E, Birkner E., “The role of the antioxidant enzymes in erythrocytes in the development of arterial hypertension among humans exposed to lead”, Biological Trace Elements Research, 130, 95-106, 2009.
  • [25] Tabrez S, and Ahmad M., “Effect of wastewater intake on antioxidant and marker enzymes of tissue damage in rat tissues: Implications for the use of biochemical markers”, Food and Chemical Toxicology, 47, 2465–2478, 2009.
  • [26] Fatima, R.A., and Ahmad M., “Certain antioxidant enzymes of Allium cepa as biomarkers for the detection of toxic heavy metals in wastewater”, Science of The Total Environment, 346, 256-273, 2005.
  • [27] Labrot, F., Ribera, D., Saint Denis, M., and Narbonne, J.F., “In vitro and in vivo studies of potential biomarkers of lead and uranium contamination: lipid peroxidation, acetylcholinesterase, catalase and glutathione peroxidase activities in three non-mammalian species”, Biomarkers, 1, 21-28, 1996.
  • [28] Bengtsson, A., Lundberg, M., Avila-Carino, J., Jacobsson, G., Holmgren, A., Scheynius, A., “Thiols decrease cytokine levels and down-regulate the expression of CD30 on human allergen-specific T helper (Th) 0 and TH2 cells”, Clinical Experimental Immunology, 123, 350-360, (2001).
  • [29] Rank, J, and Nielsen, M.H., “Genotoxicity testing of wastewater sludge using the Allium cepa anaphase-telophase chromosome aberration assay”, Mutation Research, 418, 113-119, 1998.
  • [30] Dizer, H., Wittekindt, E., Fischer, B., and Hansen, P.D., “The cytotoxic and genotoxic potential of surface water and wastewater effluents as determined by bioluminescence, umu-assays and selected biomarkers”, Chemosphere, 46, 225- 233, 2002.
  • [31] Mao, I.F., Chen, M.L., Lan, C.F., Chang, Y.P., and Chang, S.C., “Mutagenicity determination of the wastewater emitted from petrochemical industry in Taiwan”, Water Air & Soil Pollution, 76, 459-466, 1994.
  • [32] Malik, A., and Ahmad, A., “Genotoxicity of some wastewaters in India”, Environmental Toxicology, 10, 287-293, 2006.
  • [33] Barsiene, J., Andreikenaite, L., Vosyliene, M.Z., and Milukaite, A., “Genotoxicity and Immunotoxicity of Wastewater Effluents Discharged from Vilnius Wastewater Treatment Plant” Acta Zoologica Lituanica, 19, 188-196, 2009.
  • [34] Krishnamurthi, K., Saravana Devi, S., Hengstler, J.G., Hermes, M., Kumar, K., Dutta, D., Muhil Vannan, S., Subin, T.S., Yadav, R.R., and Chakrabarti, T., “Genotoxicity of sludges, wastewater and effluents from three different industries”, Archives of Toxicology, 82, 965-971, 2008.
Toplam 34 adet kaynakça vardır.

Ayrıntılar

Diğer ID JA22DF87TJ
Bölüm Araştırma Makalesi
Yazarlar

Hasan Türkez Bu kişi benim

Turgay Şişman Bu kişi benim

Ümit İncekara Bu kişi benim

Fatime Geyikoğlu Bu kişi benim

Abdulgani Tatar Bu kişi benim

M. Sait Keleş Bu kişi benim

Yayımlanma Tarihi 1 Aralık 2009
Gönderilme Tarihi 1 Aralık 2009
Yayımlandığı Sayı Yıl 2009 Cilt: 11 Sayı: 2

Kaynak Göster

APA Türkez, H., Şişman, T., İncekara, Ü., Geyikoğlu, F., vd. (2009). Erzurum’daki bir yağ fabrikasından alınan atıksu örneklerinin genotoksik ve biyokimyasal etkileri. Balıkesir Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 11(2), 55-64.
AMA Türkez H, Şişman T, İncekara Ü, Geyikoğlu F, Tatar A, Keleş MS. Erzurum’daki bir yağ fabrikasından alınan atıksu örneklerinin genotoksik ve biyokimyasal etkileri. BAUN Fen. Bil. Enst. Dergisi. Aralık 2009;11(2):55-64.
Chicago Türkez, Hasan, Turgay Şişman, Ümit İncekara, Fatime Geyikoğlu, Abdulgani Tatar, ve M. Sait Keleş. “Erzurum’daki Bir Yağ fabrikasından alınan atıksu örneklerinin Genotoksik Ve Biyokimyasal Etkileri”. Balıkesir Üniversitesi Fen Bilimleri Enstitüsü Dergisi 11, sy. 2 (Aralık 2009): 55-64.
EndNote Türkez H, Şişman T, İncekara Ü, Geyikoğlu F, Tatar A, Keleş MS (01 Aralık 2009) Erzurum’daki bir yağ fabrikasından alınan atıksu örneklerinin genotoksik ve biyokimyasal etkileri. Balıkesir Üniversitesi Fen Bilimleri Enstitüsü Dergisi 11 2 55–64.
IEEE H. Türkez, T. Şişman, Ü. İncekara, F. Geyikoğlu, A. Tatar, ve M. S. Keleş, “Erzurum’daki bir yağ fabrikasından alınan atıksu örneklerinin genotoksik ve biyokimyasal etkileri”, BAUN Fen. Bil. Enst. Dergisi, c. 11, sy. 2, ss. 55–64, 2009.
ISNAD Türkez, Hasan vd. “Erzurum’daki Bir Yağ fabrikasından alınan atıksu örneklerinin Genotoksik Ve Biyokimyasal Etkileri”. Balıkesir Üniversitesi Fen Bilimleri Enstitüsü Dergisi 11/2 (Aralık 2009), 55-64.
JAMA Türkez H, Şişman T, İncekara Ü, Geyikoğlu F, Tatar A, Keleş MS. Erzurum’daki bir yağ fabrikasından alınan atıksu örneklerinin genotoksik ve biyokimyasal etkileri. BAUN Fen. Bil. Enst. Dergisi. 2009;11:55–64.
MLA Türkez, Hasan vd. “Erzurum’daki Bir Yağ fabrikasından alınan atıksu örneklerinin Genotoksik Ve Biyokimyasal Etkileri”. Balıkesir Üniversitesi Fen Bilimleri Enstitüsü Dergisi, c. 11, sy. 2, 2009, ss. 55-64.
Vancouver Türkez H, Şişman T, İncekara Ü, Geyikoğlu F, Tatar A, Keleş MS. Erzurum’daki bir yağ fabrikasından alınan atıksu örneklerinin genotoksik ve biyokimyasal etkileri. BAUN Fen. Bil. Enst. Dergisi. 2009;11(2):55-64.