Masere sarımsak (Allium sativum Limne) ve Tunceli sarımsağı (Allium tuncelianum Kollman) yağlarının yoğun stoklanmış gökkuşağı alabalıklarının (Oncorhynchus mykiss W.) bazı kan parametrelerine ve NBT (Nitroblue Tetrazolium) seviyelerine etkileri

Yıl 2019, Cilt: 21 Sayı: 2, 716 - 723, 28.06.2019

Başar Altınterim Önder Aksu

https://doi.org/10.25092/baunfbed.637083

Cited By: 6

Öz

Bu çalışmada, % 2 oranında masere sarımsak (Allium sativum L.) ve Tunceli sarımsağı (Allium tuncelianum Koll.) yağları, ortalama ağırlıkları: 39.0 ± 1.64 gr. olan gökkuşağı alabalıklarının yemlerine ilave edilmiştir. Çalışmanın sonunda, gökkuşağı alabalıklarının kan parametreleri ve NBT (Nitroblue Tetrazolium) seviyeleri tespit edilmiştir. Sarımsak uygulaması yapılan gruplarda, MCH, P-LCR, PCT, PLT ve MPV parametrelerinde kontrol ve stres gruplarına göre istatistiksel olarak farklılık bulunmuştur (P<0.05). WBC, LYM, MID, RBC, HGB, HCT, MCV, MCHC, RDW-SD, RDW-CV ve PDW, değerleri arasında bir farklılık görülmemiştir (P> 0.05). Sarımsak uygulamaları sonucu NBT seviyeleri önemli derecede azalma göstermiştir (P<0.05). Bu durum sarımsağın hücrelerdeki oksidatif stresi azalttığını göstermektedir.

Anahtar Kelimeler

Alabalık, sarımsak yağı, Tunceli sarımsağı, NBT, kan parametreleri

Effects of oils of macerated garlic (Allium sativum Limne) and macerated Tunceli garlic (Allium tuncelianum Kollman) on some hematological parameters and NBT (NitroblueTetrazolium) levels of rainbow trout (Oncorhynchus mykiss W.) at high stocking density

Öz

In this study, 2% of macerated garlic (Allium sativum L.) and Tunceli garlic (Allium tuncelianum Kollman) oils were added to the feeds of rainbow trout with an average weight of 39.0 ± 1.64 g. At the end of the study, blood parameters and NBT (Nitroblue Tetrazolium) levels of rainbow trout were determined. A statistically significant increase in the MCH, P-LCR, PCT, PLT and MPV parameters was observed in the groups with garlic application compared to the control and stress groups (P <0.05), although there was a numerical increase in all blood parameters. There was no statisticall ysignificant difference between WBC, LYM, MID, RBC, HGB, HCT, MCV, MCHC, RDW-SD, RDW-CV and PDW levels (P> 0.05). NBT levels decreased significantly as a result of garlic applications (P <0.05). This shows that garlic reduces oxidative stress in cells.

Anahtar Kelimeler

Trout, garlic oil, Tunceli garlic, NBT, blood parameters

Kaynakça

• You, W.C., Blot, W.J., Chang, Y.S., Ershow, A., Yang, Z.T., An, Q., Henderson, B.E., Fraumeni, J.F. Jr, Wang, T.G., Allium vegetables and reduced risk of stoma chcancer, Journal of the National Cancer Institute, 81, 2, 162-4, (1989)
• Amagase, H., Petesch, B.L., Matsuura, H., Kasuga, S., Itakura, Y., Intake of garlic and its components, The Journal of Nutrition, 131, 3, 955–962, (2001).
• Erkan, N. and Bilen, G., Effect of essential oil streatment on the frozen storagestability of chub mackerel fillets, Journal für Verbraucher schutz und Lebensmittelsicherheit, 5: 101–110, (2010).
• Imai, J., Ide, N., Nagae, S., Moriguchi, T., Matsuura, H., Itakura, Y., Antioxidant and radical scavenging effects of aged garlic extract and its constituents, Planta Medica, 60, 5, 417-20 (1994).
• Chang, S.T., Chen, P.F. and Chang, S.C., Antibacteriel activity of leaf essantial oils and their consituents from Cinnamon osmophloeum, Journal of Ethanophermacology, 77, 123-127 (2001).
• Chorianopoulos, N.G., Giaouris, E.D., Skandamis, P.N., Haroutounian, S.A. and Nychas, G.J.E., Disinfectant test against monoculture and mixed-culture biofilms composed of technological, spoilage and pathogenic bacteria: Bactericidal effect of essential oil and hydrosol of Satureja thymbra and comparison with standard acidbase sanitizers, Journal of Applied Microbiology, 104, 1586–1596 (2008).
• Gulec, A.K., Altinterim, B. and Aksu, O., Determination of lethal concentration (LC50) values of Cinnamomum zeylanicum hydrosol on carp fish, Iranian Journal of Fisheries Sciences, 12, 1, 34-44, (2013).
• Altınterim, B., Kutluyer, F. and Aksu, O.. Oksijen Radikal Absorbans Kapasitesi (ORAK) Seviyeleri Farklı Bitki Masere Yağlarının Yoğun Stoklanmış Gökkuşağı Alabalıklarının (Oncorhynchus mykiss) Bazı Kan Parametrelerine Etkileri, Atatürk Üniversitesi Veteriner Bilimleri Dergisi, 13, 1, 63-69, (2018a).
• Altınterim, B., Öztürk, E., Kutluyer, F. and Aksu, O., Yeşil Çay Yağının Gökkuşağı Alabalıklarının (Oncorhynchus mykiss) Yem Değerlendirme Oranına ve Hematolojik Parametrelerine Etkileri, Atatürk Üniversitesi Veteriner Bilimleri Dergisi, 13, 2, 159-164, (2018b).
• Altınterim, B., Gulec, A.K. and Aksu O., Determination of Safety Dose of Eucalyptus camaldulensis Hydrosol on Mirror Carp (Cyprinus carpio), Fresenius Environmental Bulletin, 21, 5a, 1219-1222, (2012).
• Brace, L.D., Cardiovascular Benefits of Garlic (Allium sativum L), The Journal of Cardiovascular Nursing, 16, 4, 33-49, (2002).
• Lee, J.Y. and Gao, Y., Review of the application of garlic, Alliums sativum, in aquaculture, Journal of the World Aquaculture Society, 43, 4, 447–458, (2012).
• Talpur, A.D. and Ikhwanuddin, M., Dietary effects of garlic (Allium sativum) on haemato-immunological parameters, survival, growth, and disease resistance against Vibrio harveyi infection in Asian seabass, Latescal carifer (Bloch), Aquaculture, 364–365: 6–12, (2012).
• Freeman, F., and Kodera, Y., Garlic chemistry: stability of S-(2-Propenyl) 2-Propene-1-sulfinothiolate (Allicin) in blood, solvents and simulated physiological fluids, Journal of Agricultural and Food Chemistry, 43, 9, 2332–2338, (1995).
• Yumrutaş, Ö., Demirörs, Saygıdeğer, S., Doğan, M. The in vitro antioxidant activity of Allium tuncelianum: An endemic. Journal of Appliedbiological Sciences, 3, 3, 61-64, (2009).
• Chan, K., Yin, M. and Chao, W., Effect of diallyl trisulfide-rich garlic oil on blood coagulation and plasma activity of anticoagulation factors in rats, Food and Chemical Toxicology, 45, 3, 502-507, 2007.
• Ohaeri, O.C. and Adoga G.I. Anticoagulant Modulation of Blood Cells and Platelet Reactivity by Garlic Oil in Experimental Diabetes Mellitus, Bioscience Reports, 26, 1, DOI: 10.1007/s10540-006-9004-1, (2006).
• Farahi, A., Kasiri, M., Sudagar, M., Iraei, M.S. and Shahkolaei M.D., Effect of garlic (Allium sativum) on growth factors, some hematological parameters and body compositions in rainbow trout (Oncorhynchus mykiss), AACL Bioflux, 3, 4, 317-323, (2010).
• Shalaby, A.M.I., Khattab, Y.A., Abdel Rahman, A.M., Effects of Garlic (Allium sativum) and chloramphenicol on growth performance, physiological parameters and survival of Nile tilapia (Oreochromis niloticus), The Journal of Venomous Animals and Toxins including Tropical Diseases, 12, 2, 172-201, (2006).
• Breyer, K.E., Getchell, R.G., Cornwell, E.R. and Wooster, G.A., Efficacy of an Extract from Garlic, Allium sativum, Against Infection with the Furunculosis Bacterium, Aeromonas salmonicida, in Rainbow Trout, Oncorhynchus mykiss. Journal of The World Aquaculture Society, 46, 3, 273-282, (2015).
• Hamadouche, NA., Lazeb, H., Kaddouri, A., Guellil, H., Slimani, M. and Aoues A., Ameliorated effects of green tea extract on lead ınduced kidney toxicity in rats, Algerian J Nat Prod, 3, 130-137, (2016).
• Diab, A.S., Aly, S.M., John, G., Abde-Hadi, Y. and Mohammed, M.F., Effect of garlic, black seed and Biogen as immunostimulants on the growth and survival of Nile tilapia, Oreochromis niloticus (Teleostei: Cichlidae), and their response to artificial infection with Pseudomonas fluorescens, African Journal of Aquatic Science, 33, 1, 63–68, (2008).
• Nya, E.J. and Austin, B. Use of garlic, Allium sativum, to control Aeromonas hydrophila infection in rainbow trout, Oncorhynchus mykiss (Walbaum), Journal of Fish Diseases, 32, 11, 963-70, (2009).
• Siwicki, A.K. and Studnicka, M. The phagocyticability of neutrophils and serum lysozme activity in experimentally infected carp, Cyprinus carpio L., Journal of Fish Biology, 31, A, 57-60, (1987).
• Ruane, N.M., Carball, E.C. and Komen, J., Increased stocking density influence the acute physiological stres response of common carp Cyprinus carpio (L.), Aquaculture Research, 33, 777-784, (2002).
• Ağbaş, B., Karakuş, D., Adıgüzel, R., Keser, S. and Demir, E. Tunceli Sarımsağının (Allium tuncelianum) Toplam antioksidan Özelliklerinin ve Kuru Madde İçeriğinin Normal Sarımsak (Allium sativum) ile karşılaştırılması. Munzur Üniversitesi, Bilim ve Gençlik Dergisi, 1, 2, 50-62, (2013).