Farklı Lokasyonlarda Doğal Olarak Yetişen Sarı Kantaron (Hypericum perforatum L.) Bitki Kısımlarının Toplam Polifenol, Toplam Flavonoid ve Antioksidan Aktiviteleri

Öz

Tıbbi aromatik bitkilerin büyük bir çoğunluğu antioksidan, antimikrobiyal, antifungal, antidiyabetik, antialerjik, antikanser antienflamatuvar, antimalaryal, antitiroid ve ağrı kesici özelliklere sahip olan sekonder metabolitleri içerdiklerinden sıklıkla farklı üretim alanlarında kullanılmaktadır. Türkiye’de birçok bölgede doğal olarak yetişen ve sarı kantaron olarak da bilinen Hypericum perforatum L. bitkisi de değerli tıbbi aromatik bir bitkidir. Bu çalışmada, farklı eko-coğrafik özelliklere sahip Bayburt ve Kırklareli lokasyonlarından toplanan H. perfo-ratum L. bitkisinin çiçek, yaprak, sap ve kök kısımlarına ait antioksidan aktivitelerin belirlenmesi amaçlanmak-tadır. Farklı bölgelerde doğal olarak yetişen H. perforatum L. kısımlarının karşılaştırıldığı ilk çalışma olma özelliğine sahip bu çalışmada; en yüksek toplam fenolik madde değeri (11.87 mg GAE/g) Kırklareli lokasyo-nundan toplanan H. perforatum L. bitkisinin yaprak örneğinde, en düşük değer ise (4.49 mg GAE/g) Bayburt lokasyonundan toplanan H. perforatum L. sap örneğinde, en yüksek DPPH değeri (1.734 SC50 mg/mL) Bayburt lokasyonundan toplanan H. perforatum L. kök örneğinde, en düşük değer ise (0.259 SC50 mg/mL) Kırklareli lokasyonundan toplanan H. perforatum L. yaprak örneğinde, en yüksek FRAP değeri, (216.97 μmol Fe-SO4.7H2O/g) Kırklareli lokasyonundan toplanan H. perforatum L. yaprak örneğinde, en düşük FRAP değeri ise (60.42 μmol FeSO4.7H2O/g) ise Bayburt lokasyonundan toplanan H. perforatum L. kök örneğinde belirlenmiştir. Çalışmadan elde edilen antioksidan aktivite değerleri incelendiğinde, Kırklareli ilinde doğal olarak yetişen H. perforatum L. bitkisinin, Bayburt ilinde doğal olarak yetişene kıyasla daha tercih edilebilir olduğu sonucu ortaya çıkmaktadır.

Anahtar Kelimeler

• Tıbbi aromatik
• Hypericum
• yabani bitki
• antioksidan
• fenolik

Total Polyphenol, Total Flavonoid and Antioxidant Activities of St. John's Wort Naturally Grown in Different Locations (Hypericum perforatum L.) Plant Parts

Öz

The majority of medicinal aromatic plants are frequently used in different production areas as they contain secondary metabolites with antioxidant, antimicrobial, antifungal, antidiabetic, antiallergic, anticancer, anti-inflammatory, antimalarial, antithyroid and pain relief properties. Hypericum perforatum L., also known as St. John's Wort, which grows naturally in many regions in Turkey, is also a valuable medicinal aromatic plant. In this study, it is aimed to determine the antioxidant activities of the flower, leaf, stem and root parts of H. perforatum L. plant collected from Bayburt and Kırklareli locations with different eco-geographical characteristics. In this study, which is the first to compare the parts of H. perforatum L. grown naturally in different regions; The highest total phenolic substance value (11.87 mg GAE/g) was in the leaf sample of H. perforatum L. plant collected from Kırklareli location, and the lowest value (4.49 mg GAE/g) was in H. perforatum L. collected from Bayburt location. In the stem sample, the highest DPPH value (1.734 SC50 mg/mL) was in the H. perforatum L. root sample collected from Bayburt location, and the lowest value (0.259 SC50 mg/mL) in the H. perforatum L. leaf sample collected from the Kırklareli location, the highest FRAP value (216.97 μmol FeSO4.7H2O/g) in H. perforatum L. leaf sample collected from Kırklareli location, and the lowest FRAP value (60.42 µmol FeSO4.7H2O/g) in H. perforatum L. collected from Bayburt location. determined in the root sample. When the antioxidant activity values obtained from the study are examined, it is concluded that the naturally grown H. perforatum L. plant in Kırklareli is more preferable than the naturally grown plant in Bayburt.

Anahtar Kelimeler

• Medicinal aromatic
• Hypericum
• wild plant
• antioxidant
• phenolic

Kaynakça

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