Kuluçkalık Bıldırcın Yumurtalarına In-Ovo D3 Vitamini Enjeksiyonunun Çıkış Gücü ve Organ Ağırlığı Üzerine Etkileri

Öz

Bu çalışmanın amacı, kuluçkanın 11. ve 14. günlerinde in-ovo D3 vitamini enjeksiyonunun çıkış gücü, embriyonik ölümleri, civciv ağırlığı, kemik uzunluğu ve ağırlığı ve organ ağırlıkları üzerine etkilerini araştırmaktır. Çalışmada toplam 480 kuluçkalık bıldırcın yumurtası kullanılmıştır. Yumurtalar her bir grupta 160 adet (40 adet/alt grup) kuluçkalık yumurta olacak şekilde üç gruba rastgele dağıtılmıştır. Kuluçkanın 11 ve 14. günlerinde yumurtanın küt ucundan amniyotik sıvı içerisine test materyali enjekte edilmiştir. Birinci grup enjeksiyon yapılmayan (kontrol grubu) diğeri ise kuluçkanın 11. ve 14. günlerinde 0.2 ml Vit D3 (333 IU) enjekte edilen grup olarak düzenlenmiştir. Kuluçkanın 11. ve 14. günlerinde D3 vitaminin in-ovo enjeksiyonunun kuluçka çıkış gücünü düşürdüğü ve embriyonik ölümleri artırdığı görülmüştür (P <0.05). Bununla beraber, gruplar arasında organ ağırlıkları, kemik ağırlığı ve kemik uzunluğunda önemli bir fark olmadığı tespit edilmiştir. Sonuç olarak bu çalışmada kuluçkanın 11. ve 14. günlerinde in-ovo D3 vitamini enjeksiyonu kuluçka randımanını düşürdüğü embriyonik ölümleri arttırdığı fakat kemik ağırlık, uzunluğu ve organ ağırlığını etkilemediği görülmüştür.

Anahtar Kelimeler

• Vit D3,kuluçkalık bıldırcın yumurtası,kuluçka,çıkış gücü,organ ağırlığı

Effects of In-Ovo Injection of D3 Vitamin on Hatchability and Supply Organ Weights in Quail Hatching Eggs

Öz

The purpose of this study was to determine the effects of in-ovo injection of vitamin D3 at the day 11 and 14 of embryonic development on hatchability of fertile eggs, embryonic mortality (EM), chick weight, bone length and weight and supply organ weights of Japanese quails. A total of 480 hatching eggs were used in the study. The eggs were randomly divided into 3 groups as 160 eggs in each group with four replicates of 40 eggs each. Eggs were injected at the 11th and 14th day of incubation to deposit test material into amniotic fluid through the blunt end of the egg. The first group without injection (control), while, the second group at 11th day of incubation and third groups 14th day of incubation were injected with 0.2 ml Vit.D3 (333 IU), for per egg. The results showed that in-ovo injection of vitamin D3 on day 11 and 14 of incubation resulted in significant decreases in the hatchability and led to an increase in EM (P< 0.05). However, there was no significant difference for supply organ rate, chick and bone weight and bone length among treatment groups. In conclusion from the present study shows that injection with Vit.D3 in quail eggs with 333 IU at 11th and 14th day of incubation decreases the hatchability, leads to an increase in EM without affecting the tibia weight and length, and supply organ weights in quail eggs.

Anahtar Kelimeler

• Vit D3,Quail hatching eggs,Incubation,Hatchability of fertile eggs,organ weight

Kaynakça

1. Abdulqader, A. F., Aygün, A., Maman, A. H., Olgun, O. (2018). The effect of in-ovo injection of Lactobacilla Rhamnosus on hatching traits and growth parameters of quails. Selcuk Journal of Agriculture and Food Sciences 32, 174-8.
2. Ameenuddin, S., Sunde, M. L., DeLuca, H. F., Ikekawa, N., Kobayashi, Y. (1983). Support of embryonic chick survival by vitamin D metabolites. Archives of biochemistry and biophysics 226, 666-70.
3. Aygun, A. (2016). The effects of in-ovo injection of propolis on egg hatchability and starter live performance of japanese quails. Brazilian Journal of Poultry Science 18, 83-9.
4. Aygun, A., Sert, D., Copur, G. (2012). Effects of propolis on eggshell microbial activity, hatchability, and chick performance in Japanese quail (Coturnix coturnix japonica) eggs. Poultry Science 91, 1018-25.
5. Bello, A., Zhai, W., Gerard, P., Peebles, E. (2013a). Effects of the commercial in ovo injection of 25-hydroxycholecalciferol on broiler posthatch performance and carcass characteristics. Poultry Science 93, 155-62.
6. Bello, A, Zhai, W., Gerard, P., Peebles, E. (2013b). Effects of the commercial in ovo injection of 25-hydroxycholecalciferol on the hatchability and hatching chick quality of broilers. Poultry Science 92, 2551-9.
7. Bhanja, S., Sudhagar, M., Goel, A., Pandey, N., Mehra, M., Agarwal, S., Mandal, A. (2014). Differential expression of growth and immunity related genes influenced by in ovo supplementation of amino acids in broiler chickens. Czech J. Anim. Sci 59, 399-408.
8. Coşkun, I., Çayan, H., Yilmaz, Ö., Taskin, A., Tahtabiçen, E., Samli, H. E. (2014). Effects of in-ovo pollen extract injection to fertile broiler eggs on hatchability and subsequent chick weight. Türk Tarım ve Doğa Bilimleri Dergisi 1, 485-9.

9. Coskun, I., Korkmaz, F., Altop, A., Cayan, H., Filik, G., Sahin, A., Samli, H. E., Erener, G. (2017). The effects of in ovo pollen extract injection on growth parameters, ileal histomorphology and caecal microflora in fasted broiler chicks. Indian Journal of Animal Research 51(6), 1033-1037.
10. Coskun, I., Akkan, A., Erener, G. (2018). Effects of in ovo injection of lysine and methionine into fertile broiler (parent stock) eggs on hatchability, growth performance, caecum microbiota, and ileum histomorphology. Revista Brasileira de Zootecnia 47, 1-5.
11. DeLuca, H. F. (1992). New Concepts of Vitamin D Functions. Annals of the New York Academy of Sciences 669, 59-68.
12. Ebrahimnezhad, Y., Salmanzadeh, M., Aghdamshahryar, H., Beheshti, R., Rahimi, H. (2011) The effects of in ovo injection of glucose on characters of hatching and parameters of blood in broiler chickens. Annals of Biological Research 2:347-351.
13. Elaroussi, M. A., Deluca, H. F., Forte, L. R., Biellier, H. V. (1993). Survival of vitamin D-deficient embryos: time and choice of cholecalciferol or its metabolites for treatment in ovo. Poultry Science 72, 1118-26.
14. Elibol, O., Türkoğlu, M., Akan, M., Erol, H. (2001). Effects of ascorbic acid injection during incubation on the hatchability of large broiler eggs. Turkish Journal of Veterinary and Animal Sciences 25, 245-8.
15. Frost, T., Roland Sr, D., Untawale, G. (1990). Influence of vitamin D3, 1α-Hydroxyvitamin D3, and 1, 25-Dihydroxyvitamin D3 on eggshell quality, tibia strength, and various production parameters in commercial laying hens. Poultry Science 69, 2008-16.
16. Garabedian, M., Tanaka, Y., Holick, M., DeLuca, H. (1974). Response of intestinal calcium transport and bone calcium mobilization to 1, 25-dihydroxyvitamin Da in thyroparathyroidectomized rats. Endocrinology 94, 1022-7.
17. Gonzales, E., Cruz, C. P. D., Leandro, N. S. M., Stringhini, J. H., Brito, A. B. D. (2013). In ovo supplementation of 25 (OH) D3 to broiler embryos. Brazilian Journal of Poultry Science 15, 199-202.
18. Holbrook, J. T., Soares, J. J. (1985). The effects of estradiol and 1, 25 (OH) 2D3 on skeletal metabolism in female japanese quail. Nutrition Research 5, 729-36.
19. Ibrahim, N., Wakwak, M., Khalifa, H., Dep, B. A. (2012). Effect of in ovo injection of some nutrients and vitamins upon improving hatchability and hatching performances of ostrich embroyos. Egypt. Poult. Sci 32, 981-94.
20. Ipek, A., Sahan, U., Yilmaz, B. (2004). The effect of in ovo ascorbic acid and glucose injection in broiler breeder eggs on hatchability and chick weight. Archiv Fur Geflugelkunde 68, 132-5.
21. Kocamis, H., Yeni, Y., Kirkpatrick-Keller, D., Killefer, J. (1999). Postnatal growth of broilers in response to in ovo administration of chicken growth hormone. Poultry Science 78, 1219-26.
22. Li, C., Wang, L., Chou, C. (2005). Field evaluation of flock production performance of in ovo injection of infectious bursal disease virus immune complex vaccine in commercial broiler farms. Journal of Applied Poultry Research 14, 338-44.
23. Maiorano, G., Sobolewska, A., Cianciullo, D., Walasik, K., Elminowska-Wenda, G., Sławińska, A., Tavaniello, S., Żylińska, J., Bardowski, J., Bednarczyk, M. (2012). Influence of in ovo prebiotic and synbiotic administration on meat quality of broiler chickens. Poultry Science 91, 2963-9.
24. Miller, E., Ullrey, D., Zutaut, C., Hoefer, J., Luecke, R. (1965). Mineral balance studies with the baby pig: effects of dietary vitamin D2 level upon calcium, phosphorus and magnesium balance. The Journal of nutrition 85, 255-9.
25. Moghaddam, A., Borji, M., Komazani, D. (2014). Hatchability rate and embryonic growth of broiler chicks following in ovo injection royal jelly. British poultry science 55, 391-7.
26. Moore, R. W., Dean, C. E., Hargis, P. S., Hargis, B. M. (1994). Effects of in ovo hormone administration at day eighteen of embryogenesis on posthatch growth of broilers. Journal of Applied Poultry Research 3, 31-9.
27. Narbaitz, R., Tolnai, S. (1978). Effects produced by the administration of high doses of 1, 25-dihydroxycholecalciferol to the chick embryo. Calcified tissue research 26, 221-6.
28. Narbaitz, R., Tsang, C. (1989). Vitamin D deficiency in the chick embryo: effects on prehatching motility and on the growth and differentiation of bones, muscles, and parathyroid glands. Calcified tissue international 44, 348-55.
29. Ohta, Y., Kidd, M., Ishibashi, T. (2001). Embryo growth and amino acid concentration profiles of broiler breeder eggs, embryos, and chicks after in ovo administration of amino acids. Poultry Science 80, 1430-6.
30. Oliveira, T., Bertechini, A., Bricka, R., Kim, E., Gerard, P., Peebles, E. (2015). Effects of in ovo injection of organic zinc, manganese, and copper on the hatchability and bone parameters of broiler hatchlings. Poultry Science 94, 2488-94.
31. Reinhardt, T. A., Hustmyer, F. G. (1987). Role of vitamin D in the immune system. Journal of Dairy Science 70, 952-62.
32. Romao, J. M., de Moraes, T. G. V., Salles, R. P. R., Cardoso, W. M., Buxade, C. C. (2011). Effect of in ovo vaccination procedures on Japanese quail embryos (Coturnix japonica) and incubation performance. Ciência Animal Brasileira 12.
33. Sharma, J., Burmester, B. (1982). Resistance of Marek's disease at hatching in chickens vaccinated as embryos with the turkey herpesvirus. Avian Diseases, 134-49.
34. Sunde, M., Turk, C., DeLuca, H. (1978). The essentiality of vitamin D metabolites for embryonic chick development. Science 200, 1067-9.
35. Tako, E., Ferket, P., Uni, Z. (2004). Effects of in ovo feeding of carbohydrates and beta-hydroxy-beta-methylbutyrate on the development of chicken intestine. Poultry Science 83, 2023-8.
36. Tuan, R. S., Suyama, E. (1996). Developmental expression and vitamin D regulation of calbindin-D28K in chick embryonic yolk sac endoderm. The Journal of nutrition 126, 1308S-16S.
37. Yıldız, A. Ö., Şentürk, E. T., Aygün, A., Olgun, O. (2018). Effects of in-ovo injection of manganese on some organ weights and lengths in quail hatching eggs. Selcuk Journal of Agriculture and Food Sciences 32, 345-9.
38. Zamani, A., Shariatmadari, F., Rahimi, S., Karimi Torshizi, M. A. (2018). Effects of in ovo injection of carbohydrates, β-hydroxy-β-methylbutyrate, and vitamins on ostrich organ weight, bone characteristics, and small intestinal morphology. Canadian Journal of Animal Science 99, 116-22.
39. Zhai, W., Gerard, P., Pulikanti, R., Peebles, E. (2011). Effects of in ovo injection of carbohydrates on embryonic metabolism, hatchability, and subsequent somatic characteristics of broiler hatchlings. Poultry Science 90, 2134-43.