INVESTIGATION OF THE BIOACTIVITY AND SHELF LIFE OF YOGURT FERMENTED WITH PERGA

Yıl 2025, Cilt: 5 Sayı: 2, 59 - 71, 19.12.2025

Sibel Silici , Burcu Budur , Kevser Karaman

Öz

In this study, raw milk obtained from a dairy farmer raising livestock in the Kayseri-Gesi region was fermented using traditional methods using yogurt culture and various amounts of perga additive. Perga was added to the samples at different rates (0.01, 0.02, 0.03, and 0.04%). Color, titratable acidity, pH, water-holding capacity, total phenolic content, and DPPH antioxidant capacity, were performed on yogurt samples on days 1, 4, 7, 14, and 21 of storage. Protein content was determined on the first day of storage. The results showed that the pH of the yogurt decreased as the concentration of perga added increased, but the pH difference decreased as the days of storage increased. The pH values in the samples ranged from 3.85 to 4.17. The water-retention capacity of the yogurt increased with the addition of perga and the days of storage. Titratable acidity was found to be within normal limits at all tested concentrations. No difference was observed between the samples in terms of color. Perga had no significant effect on total phenolic content, DPPH antioxidant capacity. The addition of low amounts of perga also did not significantly and negatively alter the yogurt's bioactivity or shelf life.

Anahtar Kelimeler

perga , yoğurt , acidity , bioactivity

Etik Beyan

No

Destekleyen Kurum

Erciyes University Scientific Research Projects Unit

Proje Numarası

FYL-2022-11846.

Teşekkür

This study was supported by Erciyes University Scientific Research Projects Unit with project code FYL-2022-11846.

PERGA İLE FERMENTE EDİLEN YOĞURDUN BİYOAKTİVİTESİ VE RAF ÖMRÜNÜN ARAŞTIRILMASI

Öz

Bu çalışmada Kayseri-Gesi Bölgesinde hayvancılık yapan bir sütçüden elde edilen çiğ süt yoğurt kültürü ve farklı oranlarda perga eklentisi ile geleneksel yöntemlerle mayalanmıştır. Perga örnekleri farklı oranlarda (%0.01, 0.02, 0.03 ve 0.04) eklenmiştir. Depolamanın 1,4,7,14 ve 21. günlerinde yoğurt örneklerinde renk, titrasyon asitliği, pH, su tutma kapasitesi, toplam fenolik madde, DPPH antioksidan kapasite tayini yapılmıştır. Elde edilen sonuçlara göre, eklenen perganın konsantrasyonu arttıkça yoğurdun pH değeri düşmüş ancak depolama günleri arttıkça pH farkı azalmıştır. Örneklerde pH değerleri 3.85-4.17 arasında değişkenlik göstermiştir. Perga eklentisi ve depolama günleri arttıkça yoğurtta su tutma kapasitesi artmıştır. Test edilen tüm konsantrasyonlarda titrasyon asitliği normal değerlerde tespit edilmiştir. Renk açısından da örnekler arasında fark belirlenmemiştir. Toplam fenolik madde ve DPPH antioksidan kapasite değerleri açısından perganın önemli etkisi olmamıştır. Düşük oranda perga ilavesi yoğurdun biyoaktivitesi ve raf ömründe de önemli ve olumsuz bir değişikliğe sebep olmamıştır.

Anahtar Kelimeler

perga , yoğurt , asidite , biyoaktivite

Etik Beyan

yok

Destekleyen Kurum

Erciyes Üniversitesi Bilimsel Araştırma Projeleri Birimi

Proje Numarası

FYL-2022-11846.

Teşekkür

Bu çalışma Erciyes Üniversitesi Bilimsel Araştırma Projeleri Birimi tarafından desteklenmiştir (proje no: FYL-2022-11846)

Kaynakça

• 1. Kieliszek, M., Kolotylo, V., Mikołajczuk-Szczyrba, A., Giurgiulescu, L., & Kot, A.M. et al. (2021). Isolation and identification of new yeast strains from beebread. Carpathian Journal of Food Science & Technology, 13(1), 207-213.
• 2. Kaplan, M., Karaoğlu, Ö., & Silici, S. (2019). An evaluation on bee bread: chemical and palynological analysis. Mellifera, 19 (1), 21-29.
• 3. Dranca, F., Ursachi, F., & Oroian, M. (2020). Beebread: physicochemical characterization and phenolic content extraction optimization. Foods 9 (10), 1358.
• 4. Doğanyiğit, Z., Okan, A., Kaymak, E., Pandır, D., & Silici S. (2020). Investigation of protective effects of apilarnil against lipopolysaccharide induced liverinjury in ratsvia TLR 4/ HMGB-1/ NF-κBpathway. Biomedicine and Pharmacotherapy, 125,109967
• 5. Caner, A., Onal, M.G., & Silici, S. (2021) The effect of bee bread (Perga) with chemotherapy on MDA-MB-231 cells. Molecular Biology Reports, 48, 2299–2306. https://doi.org/10.1007/s11033-021-06259-3
• 6. Nagai, T., & Inoue, R. (2004). Preparation and the functional properties of water extractand alkaline extract of royal jelly. Food Chemistry, 84(2), 181-186.
• 7. Bankole, A.O., Irondi, E.A., Awoyale, W., & Ajani, E.O. (2023). Application of natural and modified additives in yogurt formulation: types, production, and rheological and nutraceutical benefits. Frontiers Nutrition,10, 1257439. doi: 10.3389/fnut.2023.1257439.
• 8. Ibhaze, G.A., Akinbanjo, D.T., & Jacob, G.T. (2021). Evaluation of set yoghurt quality enhanced with selected indigenous fruits. International Journal of Trend in Scientific Research and Development, 5,690–9.
• 9. Sheikh, S., Siddique, F., Ameer, K., Ahmad, R.S., & Hameed, A. et al. (2023). Effects of white mulberry powder fortification on antioxidant activity, physicochemical, microbial and sensorial properties of yogurt produced from buffalo milk. Food Science and Nutrition, 11, 204–15. doi: 10.1002/fsn3.3053,
• 10. Dabija, A., Codina, G.G., Gatlan, A-M., & Rusu, L. (2018). Quality assessment of yogurt enriched with different types of fibers. CyTA-J. Food,16, 859–67. doi: 10.1080/19476337.2018.1483970.
• 11. Abed, I.J., Hussein, A.R., Abdulhasan, G.A., & Dubaish, A.N. (2022). Microbiological effect of lemongrass Cymbopogon citratus and spearmint Mentha spicata essential oils as preservatives and flavor additives in yogurt. Iraqi Journal of Science, 63, 2839–49. doi: 10.24996/ijs.2022.63.7.8
• 12. Szołtysik, M., Kucharska, A.Z., Dąbrowska, A., Zięba, T., & Bobak, L. et al. (2021). Effect of two combined functional additives on yoghurt properties. Foods, 10,1159. doi: 10.3390/foods10061159
• 13. Zhao, Y., Fu, R., & Li, J. (2020). Effects of the β-glucan, curdlan, on the fermentation performance, microstructure, rheological and textural properties of set yogurt. LWT- Food Science and Technology, 128, 109449. doi: 10.1016/j.lwt.2020.109449
• 14. Khubber, S., Chaturvedi, K., Thakur, N., Sharma, N., & Yadav, S.K. (2021). Low-methoxyl pectin stabilizes low-fat set yoghurt and improves their physicochemical properties, rheology, microstructure and sensory liking. Food Hydrocolloids, 111, 106240. doi: 10.1016/j.foodhyd.2020.106240
• 15. Barrantes, E., Tamime, A.Y., & Sword, A.M. (1994). Production of low-calorie yoghurt using skim milk powder and fat substitutes. 3. Microbiological and organoleptic qualities. Milchwissenschaft, 49, 205–208.
• 16. Yılmazer, M.S., Dirim, S.N., Pinto, D.D., & Ertekin, F.K. (2014). Yoghurt with candied chesnut: freze drying, physical and rheological behavior. Journal of Food Science and Technology, 51(12), 3949- 3955.
• 17. Wang, H., Zhang, Y., Han, Y., Hou, J., & Zuo, Y. et al. (2025). The physicochemical, sensory, and functional properties of yogurt containing millet and milk. Foods, 14, 3491. https://doi.org/10.3390/foods14203491
• 18. Feizollahi, S., & Kabakcı, D. (2025). Comparison of the bioactive content and antioxidant capacity of bee bread samples from different beehives. Turkish Journal of Agriculture - Food Science and Technology, 13(11), 3399–3403. https://doi.org/10.24925/turjaf.v13i11.3399
• 19. Adebo, O.A., & Gabriela Medina-Meza, I. (2020). Impact of fermentation on the phenolic compounds and antioxidant activity of whole cereal grains: a mini review. Molecules, 19, 25(4),927. doi: 10.3390/molecules25040927.
• 20. Marshall, V.M., Cole, W.M., & Vega, J.R. (1982). A yoghurt-like product made by fermenting ultrafiltered mik containing elevated whey proteins with Lactobacillus acidophilus. Journal of Dairy Research, 49(4), 665-670.
• 21. Cemeroğlu, B. (2007). Gıda analizlerinde genel yöntemler. Gıda Analizleri (Food Analysis), Ed: Cemeroğlu, B., GTD Yayınları, (34), 45–128.
• 22. Kurtuldu, O., & Özcan, T. (2018). Effect of beta glucan on the properties of probiotic set yoghurt with Bifidobacterium animals subsp. Lactis strain Bb-12. International Journal of Dairy Technology, 71(1), 157-166.
• 23. Wu, H., Hulbert, G.J., & Mount, J. (2000). Effects of ultrasound on milk homogenization and fermentation with yoghurt starter. Innovative Food Science and Emerging Technologies, 1(3), 211-218.
• 24. Waterhouse, D.S., Zhou, J., & Wadhwa, S.S. (2013). Drinking yoghurts with berry polyphenols added before and after fermentation. Food Control, 32(2), 450-460.
• 25. Yoon, J.W., Ahn, S.I., Jhoo, J.W., & Kim, G.Y. (2019). Antioxidant activity of yogurt fermented at low temperature and its anti-inflammatory effect on dss-induced colitis in mice. Food Science of Animal Resources, 39(1),162-176. doi: 10.5851/kosfa.2019.e13.
• 26. Deshwal, G. K., Tiwari, S., Kumar, A., Raman, R. K., & Kadyan, S. (2021). Review on factors affecting and control of post-acidification in yoghurt and related products. Trends in Food Science & Technology, 109, 499-512.
• 27. Dincel S. (2012). Chemical and rheological properties of yoghurt produced by lactic acid cultures isolated from traditional Turkish yoghurt. Middle East Technical University Master Thesis.
• 28. Citta, A., Folda, A., Scutari, G., Bindoli, A., & Bellamio, M. et al. (2017). Oxidative changes in lipids, proteins and antioxidants in yogurt during the shelf life. Food Sience & Nutrition, 1079-1087.
• 29. Keyvan, N., & Yurdakul, O. (2024). Bee bread boosts probiotic Yoghurt: Unveiling the impact on physiochemical, microbiological, and sensory attributes. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 71(4), 471-480.
• 30. Rashwan, A.K., Osman, A.I. & Chen, W. (2023). Natural nutraceuticals for enhancing yogurt properties: a review. Environmental Chemistry Letters, 21, 1907–1931 (2023).
• [31] Cosme-Alonso, V., Martinez-Lifshitz, M. E., & Mani-Lopez, E. (2023). Yogurt supplemented with bee pollen: Physicochemical and sensory properties, and in vitro pollen digestibility. Journal of Agricultural, Food Science & Biotechnology, 1(3), 224-231.