Bazı Antibiyotiklerin Koyun Dalak Glutatyon S-Transferaz Enzimi Üzerine İn Vitro Etkilerinin İncelenmesi

Yıl 2023, , 190 - 196, 28.06.2023

Ömer Faruk Çiftci , Songül Ünüvar , Yusuf Temel

https://doi.org/10.58605/bingolsaglik.1281161

Öz

Glutatyon S-transferazlar (GST) hücre içi detoksifikasyon mekanizmasında görev alan multigen ailesine sahip izoenzimlerdir. GST'ler, faz II sistemi ile endojen ve eksojen kaynaklı kimyasalların toksik etkisini azaltır. GST izoenzimleri tarafından katalize edilen reaksiyonlarda, indirgenmiş glutatyonun (GSH) tiyol grubu sayesinde çeşitli elektrofillerle konjugasyon oluşturur. GST izoenzimleri hücrede sitozol ya da mikrozomlarda bulunabilir.
Bu çalışmada koyun dalak dokusundan sitozolik glutatyon S-transferaz enzimi, homojenat hazırlanması ve glutatyon agaroz afinite kromatografisi kullanılarak saflaştırıldı. İkinci aşamada saflaştırılan GST enzimi üzerine sefazolin sodyum, sefuroksim sodyum, sefaperazon sodyum, ampisillin, gentamisin, klindamisin ve tylosin antibiyotiklerin in vitro etkileri incelendi ve inhibisyon etkisi gösteren antibiyotikler için IC50 değerleri hesaplandı. Antibiyotiklerden sefaperazon sodyum, sefazolin sodyum, sefuroksim sodyum, klindamisin ve gentamisin, enzim aktivitesi üzerinde inhibisyon etkisi gösterirken ampisillinin aktivasyon etkisi gösterdiği tespit edildi. Tylosinin ise 0,545-5,45 mM aralığında enzim üzerinde herhangi etkiye sahip olmadığı belirlendi.

Anahtar Kelimeler

Antibiyotik, Enzim, Glutatyon S-transferaz, İnhibisyon

Kaynakça

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