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Bazı Antibiyotiklerin Koyun Dalak Glutatyon S-Transferaz Enzimi Üzerine İn Vitro Etkilerinin İncelenmesi

Yıl 2023, , 190 - 196, 28.06.2023
https://doi.org/10.58605/bingolsaglik.1281161

Öz

Glutatyon S-transferazlar (GST) hücre içi detoksifikasyon mekanizmasında görev alan multigen ailesine sahip izoenzimlerdir. GST'ler, faz II sistemi ile endojen ve eksojen kaynaklı kimyasalların toksik etkisini azaltır. GST izoenzimleri tarafından katalize edilen reaksiyonlarda, indirgenmiş glutatyonun (GSH) tiyol grubu sayesinde çeşitli elektrofillerle konjugasyon oluşturur. GST izoenzimleri hücrede sitozol ya da mikrozomlarda bulunabilir.
Bu çalışmada koyun dalak dokusundan sitozolik glutatyon S-transferaz enzimi, homojenat hazırlanması ve glutatyon agaroz afinite kromatografisi kullanılarak saflaştırıldı. İkinci aşamada saflaştırılan GST enzimi üzerine sefazolin sodyum, sefuroksim sodyum, sefaperazon sodyum, ampisillin, gentamisin, klindamisin ve tylosin antibiyotiklerin in vitro etkileri incelendi ve inhibisyon etkisi gösteren antibiyotikler için IC50 değerleri hesaplandı. Antibiyotiklerden sefaperazon sodyum, sefazolin sodyum, sefuroksim sodyum, klindamisin ve gentamisin, enzim aktivitesi üzerinde inhibisyon etkisi gösterirken ampisillinin aktivasyon etkisi gösterdiği tespit edildi. Tylosinin ise 0,545-5,45 mM aralığında enzim üzerinde herhangi etkiye sahip olmadığı belirlendi.

Kaynakça

  • Aksoy, M., Ozaslan, M. S., & Kufrevioglu, O. I. (2016). Purification of glutathione S-transferase from Van Lake fish (Chalcalburnus tarichii Pallas) muscle and investigation of some metal ions effect on enzyme activity. Journal of Enzyme Inhibition and Medicinal Chemistry, 31(4), 546–550. https://doi.org/10.3109/14756366.2015.1046063
  • Pljesa-Ercegovac, M., Savic-Radojevic, A., Matic, M., Coric, V., Djukic, T., Radic, T., & Simic, T. (2018). Glutathione transferases: Potential targets to overcome chemoresistance in solid tumors. International Journal of Molecular Sciences, 19(12). https://doi.org/10.3390/ijms19123785
  • Temel, Y., & Taysi, M. Ş. (2018). The Effect of Mercury Chloride and Boric Acid on Rat Erythrocyte Enzymes. Biological Trace Element Research, 177–182. https://doi.org/10.1007/s12011-018-1601-x
  • Temel, Y., Koçyigit, U. M., Taysi, M. S., Gökalp, F., Gürdere, M. B., Budak, Y., … Çiftci, M. (2018). Purification of glutathione S-transferase enzyme from quail liver tissue and inhibition effects of (3aR,4S,7R,7aS)-2-(4-((E)-3-(aryl)acryloyl)phenyl)-3a,4,7,7a-tetrahydro-1H-4,7-methanoisoindole-1,3(2H)-dione derivatives on the enzyme activity. Journal of Biochemical and Molecular Toxicology. https://doi.org/10.1002/jbt.22034
  • Taysi, M. Ş., & Temel, Y. (2021). Glutathione S-transferase: Purification and Characterization from Quail (Coturnix coturnix japonica) Liver and the Impact of Some Metal Ions on Enzyme Activity. BioNanoScience, 11(1), 91–98. https://doi.org/10.1007/s12668-020-00811-4
  • Aybek, H., Temel, Y., Ahmed, B. M., Ağca, C. A., & Çiftci, M. (2020). Deciphering of The Effect of Chemotherapeutic Agents on Human Glutathione S-Transferase Enzyme and MCF-7 Cell Line. Protein & Peptide Letters, 27(9), 888–894. https://doi.org/10.2174/0929866527666200413101017
  • Özaslan, M. S., Demir, Y., Aslan, H. E., Beydemir, Ş., & Küfrevioğlu, Ö. İ. (2018). Evaluation of chalcones as inhibitors of glutathione S-transferase. Journal of Biochemical and Molecular Toxicology, 32(5), 1–6. https://doi.org/10.1002/jbt.22047
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  • Singh, R. R., & Reindl, K. M. (2021). Glutathione S-Transferases in Cancer.
  • Lebda, M., & Taha, N. (2012). Purification and Characterization of Glutathione-S-Transferase from Rat′s Liver: Effect of Carbon Tetrachloride and Camel′s Milk. Journal of Chromatography & Separation Techniques, 03(04). https://doi.org/10.4172/2157-7064.1000133
  • Hutchings, M., Truman, A., & Wilkinson, B. (2019). Antibiotics: past, present and future. Current Opinion in Microbiology, 51(Figure 1), 72–80. https://doi.org/10.1016/j.mib.2019.10.008
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  • Temel, Y., Ayna, A., Hamdi Shafeeq, I., & Ciftci, M. (2020). In vitro effects of some antibiotics on glucose-6-phosphate dehydrogenase from rat (Rattus norvegicus) erythrocyte. Drug and Chemical Toxicology, 43(2), 219–223. https://doi.org/10.1080/01480545.2018.1481083
  • Ayna, A., Khosnaw, L., Temel, Y., & Ciftci, M. (2021). Antibiotics as Inhibitor of Glutathione S-transferase: Biological Evaluation and Molecular Structure Studies. Current Drug Metabolism, 22(4), 308–314. https://doi.org/10.2174/1389200222666210118102700
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  • Balcı, N., Türkan, F., Şakiroğlu, H., Aygün, A., & Şen, F. (2019). Purification and characterization of glutathione S-transferase from blueberry fruits (Vaccinium arctostaphylos L.) and investigated of some pesticide inhibition effects on enzyme activity. Heliyon, 5(4). https://doi.org/10.1016/j.heliyon.2019.e01422
  • Temel, Y., & Kocyigit, U. M. (2017). Purification of glucose-6-phosphate dehydrogenase from rat ( Rattus norvegicus ) erythrocytes and inhibition effects of some metal ions on enzyme activity, (March). https://doi.org/10.1002/jbt.21927
  • Temel, Y., Bozkuş, T., Karagözoğlu, Y., & Çİftcİ, M. (2017). Glutatyon Redüktaz ( GR ) Enziminin Japon Bıldırcın ( Coturnix coturnix japanica ) Eritrositlerinden Saflaştırılması ve Karakterizasyonu Purification and Characterization of Glutathion Reductase Enzyme From Japanese Quail ( Coturnix coturnix japanica ) Er, 7(3), 143–150.
  • Mirza Ahmed, B., Temel, Y., Çiftci, M. (2019). Purification and characterization glutathione S-transferase enzyme from quail (Coturnix, coturnix japonica) heart and investigation the effect of some metal ions on enzyme activity, Cumhuriyet Science Journal, 40, 802–812.
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Toplam 25 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Konular Sağlık Kurumları Yönetimi
Bölüm Araştırma Makaleleri
Yazarlar

Ömer Faruk Çiftci 0000-0002-4214-4382

Songül Ünüvar 0000-0001-8454-490X

Yusuf Temel 0000-0001-8148-3718

Yayımlanma Tarihi 28 Haziran 2023
Gönderilme Tarihi 11 Nisan 2023
Yayımlandığı Sayı Yıl 2023

Kaynak Göster

Vancouver Çiftci ÖF, Ünüvar S, Temel Y. Bazı Antibiyotiklerin Koyun Dalak Glutatyon S-Transferaz Enzimi Üzerine İn Vitro Etkilerinin İncelenmesi. BÜSAD. 2023;4(1):190-6.