Elma Kabuğu Ekstresi Temelli Organik-İnorganik Nançiçek ile İçsel Peroksidaz Benzeri ve Antimikrobiyal Aktiviteler

Abstract

Amaç: Bu çalışmada, flavonoid açısından zengin elma kabuğu özütü ve Cu2+ iyonlarının sırasıyla organik ve inorganik bileşenler olarak hareket ettiği hibrit organik-inorganik nanoçiçek (NF) sentezini geliştirdik. Metod: Elma kabuğu özütü temelli NÇ'ler (Ek-NÇ'ler) peroksidaz benzeri ve antimikrobiyal aktiviteler gösterdi. Ek-NÇ'lerin morfolojiyi (boyut ve şekil) izlemek için Taramalı Elektron Mikroskopu (SEM), eğilme ve gerilme titreşimleri için Fourier Dönüşümlü Kızılötesi Spektroskopisi (FTIR) ve Ek-NÇ'lerin kristal yapısını açıklamak için X-ışını kırınımı (XRD) ile karakterize edildi. Bulgular: Katalitik aktiviteler açısından, Ek-NÇ'ler hidrojen peroksit (H2O2) varlığında Fenton reaksiyonu yoluyla guaiacolün (2-metoksi fenol) 3,3-dimetoksi-4,4-difenokinona oksidasyonunu katalize etti. Hem Fenton reaksiyonundan hem de Ek-NÇ'lerin gözenekli morfolojisinden yararlanılarak, antimikrobiyal aktiviteleri Escherichia coli (E. coli), Staphylococcus aureus (S. aureus) ve Candida albicans (C. albicans)'a karşı test edildi. Sonuç: Ek-NÇ'lerin H2O2 varlığında tüm mikroorganizmaların neredeyse %99'unu öldürerek büyük antimikrobiyal aktivite gösterdiğini belirtmekte fayda var. Sonuçlar oldukça ümit verici ve Ek-NÇ'lerin katalitik aktivite ve antimikrobiyal çalışmalar için ideal aday olabileceğini iddia ediyoruz.

Keywords

• Elma Kabuğu Ekstraktı
• Organik-İnorganik Nanoçiçek
• Peroksidaz Benzeri Aktivitesi
• Fenton Reaksiyonu
• Antimikrobiyal Aktivite

Apple peel extract based formation of organic-inorganic nanoflower with ıntrinsic peroxidase mimic and antimcrobial activities

Abstract

IPurpose: In this study, we developed synthesis of hybrid organic-inorganic nanoflower (NF) composed of flavonoid-rich apple peel extract and Cu2+ ions acted as organic and inorganic components, respectively. The apple peel extract based NFs (Ap-NFs) showed peroxidase mimic and antimicrobial activities. Method: The Ap-NFs were characterized with by Scanning Electron Microscope (SEM) for monitoring morphology (size and shape), Fourier transform Infrared Spectroscopy (FTIR) for bending and stretching vibrations and X-Ray Diffraction (XRD) for elucidation of crystal structure of Ap-NFs. Findings: In terms of the catalytic activities, Ap-NFs catalyzed oxidation of guaiacol (2-methoxy phenol) to 3,3-dimethoxy-4,4-diphenoquinone in the presence of hydrogen peroxide (H2O2) through the Fenton reaction. Benefiting from both the Fenton reaction and porous morphology of Ap-NFs, their antimicrobial activities were tested towards, Escherichia coli (E. coli), Staphylococcus aureus (S. aureus) and Candida albicans (C. albicans). Conclusion: It is worthy to mention that, the Ap-NFs exhibited great antimicrobial activity in the presence of H2O2 by killing almost 99% of all microorganisms. The results are quite promising and we claim that Ap-NFs can be ideal candidate for catalytic activity and antimicrobial studies.

Keywords

• Apple Peel Extract
• Organic-Inorganic Nanoflower
• Peroxidase Mimic Activity
• Fenton Reaction
• Antimcrobial Activity

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